Seroprevalence of Brucellosis in Holstein-Friesian and Indigenous Cattle Breeds of Sindh Province, Pakistan

Abstract

| Brucellosis is considered to be one of the most widespread zoonosis in the world. A comparative study on the seroprevalence of brucellosis in indigenous cattle breeds and exotic cattle (Holstein-Friesian) was carried out in district Hyderabad, Sindh, Pakistan. A total of 500 serum samples, 100 each from Red Sindhi, Thari, Kankrej, Bhagnari and Holstein-Friesian breeds were collected from both male and female animals and examined by Rose Bengal plate test (RBPT), serum agglutination test (SAT) and Competitive Enzyme Linked Immunosorbent Assay (c-ELISA). The overall prevalence of brucellosis in cattle was determined as 25, 23.2 and 11.8% by RBPT, SAT and c-ELISA respectively. However, highest prevalence of brucellosis was recorded in Holstein-Friesian (RBPT: 35%; SAT: 33%; c-ELISA: 17%), followed by Red Sindhi (RBPT: 28%; SAT: 26%; c-ELISA: 15%), Thari (RBPT: 24%; SAT: 23%; c-ELISA: 9%), Kankrej (RBPT: 20%; SAT: 18%; c-ELISA: 10%) and Bhagnari (RBPT: 18%; SAT: 16%; c-ELISA: 8%). The sex-wise comparison of seropositive animals indicated that the prevalence of brucellosis was 26.88% in females and 8% in males by both, RBPT and SAT. Furthermore somewhat lower prevalence (12.22%) of brucellosis was demonstrated in female cattle by c-ELISA. The effect of age on the seroprevalence of brucellosis in cattle of Sindh province was also studied. Of the 250 sera examined from cattle ≤ 4 years of age, 51(20.4%), 49(19.6%) and 26 (10.4%) were found positive by RBPT, SAT and c-ELISA respectively. While, of the 250 sera of ˃ 4 years of age 74 (29.6%), 66 (26.4%) and 33 (13.2%) were found positive by RBPT, SAT and c-ELISA, respectively. It was concluded from the present study that exotic cattle (Holstein-Friesian) have higher seroprevalence of brucellosis compared to local cattle breeds amongst which highest prevalence was recorded in Red Sindhi and lowest in Bhagnari breed. Further, among the sero-diagnostic techniques applied during present investigation, the c-ELISA was found as superior technique to determine brucellosis in cattle.

Prevalence of Subclinical Mastitis in Dairy Goats Caused by Bacterial Species

“…Whereas in present study the prevalence was recorded higher (52.63%) in right quarters as compared to left side quarters (47.36%). This inconsistency in results could be due to the breed differences (Mangi et al, 2015), as in present study dairy goats of different breeds were examined for prevalence of subclinical mastitis.…”

Isolation of Mycobacterium bovis from Milk and Nasal Discharge Samples of Cattle from Hyderabad and Tando Allahyar Districts

“…(Hussin et al, 2016;Peter et al, 2015). Moreover, it has been revealed that different species/breeds have different susceptibility/resistant levels for bacterial pathogens (Mangi et al, 2015). A higher prevalence in Hyderabad district could be due to that it is a most populous city of Sindh province after Karachi and also a border city where animals brought from several neighboring animal markets for sell .…”

Occurrence of mastitis and methicillin resistant <i>Staphylococcus aureus</i> in goats in Zaria, Kaduna State, Nigeria

“…This is in agreement with the reports of Pirzada et al (2016) in Pakistan who reported 52.63% prevalence for the right udder halves and 47.36% for the left udder halves. This was thought to be due to the greater milk production capacity of the right udder halves (Mangi et al 2015). Staphylococcus aureus has been known to show multiple antimicrobial resistance patterns.…”