Isolation of Mycobacterium bovis from Milk and Nasal Discharge Samples of Cattle from Hyderabad and Tando Allahyar Districts

Leghari, Ambreen; Kamboh, Asghar Ali; Dewani, Parkash; Abro, Shahid Hussain; Umrani, Aslam Parvez; Malhi, Kanwar Kumar; Rajput, Zahid Iqbal; Lakho, Shakeel Ahmed; Bano, Iqra; Shah, Jan Mohammad

doi:10.14737/journal.jahp/2016/4.4.105.110

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…In agreement to our results previous studies have also indicated the non significant associations of age for prevalence of bacterial diseases in farm animals (Kiros et al, 2016;Leghari et al, 2016). Shabbir et al (2015) have also reported no association of risk factors with prevalence of B. anthracis DNA in soil samples analyzed from Lahore, Pakistan.…”

Section: Journal Of Animal Health and Productionsupporting

confidence: 92%

Occurrence of Anthrax Spores in Small Ruminants Hair/Wool in District Tharparkar, Sindh

Rajput¹,

Kamboh²,

Dewani³

et al. 2017

J. Anim. Health Prod.

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| Bacillus anthracis (B. anthracis) is a spore-forming bacteria that causes anthrax in animals, including small ruminants. The present study, for the first time investigated the B. anthracis spores contamination in small ruminant's wool/hair in district Tharparkar, Pakistan. Randomly selected sheep and goats (n=40 each) were used for sample collection from different body parts i.e., abdomen, neck + face, fore limbs and hind limbs. Overall results showed that 28% samples were contaminated with anthrax spores. A moderately high (P > 0.05) incidence of anthrax spores was observed in samples collected from abdomen (35%), followed by neck + face (30%), fore limbs (25%) and hind limbs (20%) respectively. Sheep wool (30%) has a higher contamination level (P < 0.05) than goat hair (25%). In sheep, the highest prevalence was observed in 2 years of age group i.e., 4/8 (50%), whereas, 3/8 (37.5%), 3/12 (25%) and 2/12 (16.6%) samples were positive in age groups of 2 ½, 3 and 4 years respectively. However, in goats the prevalence was recorded as 6/12 (50%), 3/12 (25%) and 1/8 (12.5%) in age groups of 2, 2 ½ and 3 years respectively, whereas, anthrax spores were not found in goats of 4 years age-group. These results indicated that wool/hair of small ruminants harbor B. anthracis spores. Moreover, sheep wool harbored relatively higher spore numbers as compared to goat hair.

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Section: Journal Of Animal Health and Productionsupporting

confidence: 92%

Occurrence of Anthrax Spores in Small Ruminants Hair/Wool in District Tharparkar, Sindh

Rajput¹,

Kamboh²,

Dewani³

et al. 2017

J. Anim. Health Prod.

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“…In present study, 2.5% milk samples showed growth on Lowenstein Jensen medium. Similar level of TB prevalence was declared by Qamar and Azhar, (2013) in their study from Lahore city; and also in our earlier study conducted in Tando Allahyar and Hyderabad in cattle (Leghari et al, 2016). These findings and results of current study suggested the apparently healthy animals as major reservoir for transmission of infection to other livestock animals.…”

Section: Prevalence Of Bovine Tb In Relation To Various Factorssupporting

confidence: 92%

“…Moreover, control of bovine tuberculosis infection in buffaloes of Pakistan practically becomes unrealistic. Most of the work is confined to the prevalence of tuberculosis in cow and detected by tuberculin tests (Leghari et al, 2016). The present study was designed to investigate not only the prevalence of the disease but also the epidemiological factors of disease in buffaloes.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Malhi

Kamboh

Kumar

et al. 2018

IJAR

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An investigation in buffaloes was carried out in Hyderabad and Tando Allahyar districts, Sindh province, Pakistan to determine the prevalence of bovine tuberculosis. The buffaloes (n=120) were first screened through single intradermal tuberculin test (SITT), then their sera were used for enzyme-linked immunosorbent assay (ELISA). Attempts were further made to isolate the Mycobacterium bovis organism from the milk samples using traditional culture test. Overall prevalence of 4.16%, 8.33% and 2.5% was recorded by SITT, ELISA and culture test respectively. A somewhat higher prevalence was recorded in Tando Allahyar district (SITT 6.66%; ELISA 10%; culture test 5%) as compared to Hyderabad district (SITT 1.66%; ELISA 6.66%; culture test 0%). Statistical analysis did not show any association (P> 0.05) of herd size, sex, age, milk yield and farming type with the prevalence of the disease. Whereas, SITT showed a significant (P Less than 0.05) association of sex, age and milk yield with the prevalence of the disease in Tando Allayer district. The results of present study revealed that bovine tuberculosis is present in apparently healthy buffalo herds of Hyderabad and Tando Allayer districts. Moreover, infected animals shed the M. bovis pathogen in milk that could be a potential hazard to public health.

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“…Episodes of food borne illness are reported in the Pakistan, but due to lack of investigation network, the exact magnitude of the problem in the country remains unknown, particularly in the Tando-Allahyar region. The majority of the population consume meat slaughtered and sold in small local shops, where the maintenance of hygiene is always questionable (Akhtar, 2015;Leghari et al, 2016 (Cho et al, 2012;Javed, 2016;Kamboh et al, 2017).…”

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confidence: 99%

Bacterial Contamination of Raw Meat and Butchers Equipment in Retail Shops in Tando-Allahyar, Pakistan

Bughti¹,

Abro²,

Kamboh³

et al. 2017

J. Anim. Health Prod.

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| The present study was performed to evaluate the aerobic bacterial contamination in meat and butchers' equipment in retail shops in Tando-Allahyar, Pakistan. A total of 100 samples (40 beef, 40 mutton and 20 butchers' meat-cutting equipment) were obtained from butcher shops from local vendors of different regions of Tando-Allahyar. The samples were collected randomly in sterile labelled polythene bags or bijoux bottles individually and were transferred to laboratory at 4 o C within 3-4 hours of collection. All the samples were subjected to aerobic plate count method that followed by the standard method of isolation and identification. The bacterial load in (g-1 ) meat samples was recorded higher (p< 0.05) in beef samples (4.1x10 9 ) than mutton (3.9x10 7 ) and butchers' meat cutting equipment samples (3.7x10 6 ). The bacterial organisms including Escherichia coli, Bacillus cereus, Staphylococcus aureus, Shigella dysenteriae and Salmonella enteritidis were recorded as individual or mixed contaminants in meat and meat-cutting equipment samples. From the results, it could be concluded that in Tando-Allahyar, meat samples of cattle beef and sheep mutton, as well as butcher's meat-cutting equipment are highly contaminated. The contamination level of aerobic spoilage bacteria was found higher in cattle beef as compared to sheep mutton.

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Isolation of Mycobacterium bovis from Milk and Nasal Discharge Samples of Cattle from Hyderabad and Tando Allahyar Districts

Abstract: The pathogen may shed through many ways as in saliva,

Cited by 5 publications

References 13 publications

Occurrence of Anthrax Spores in Small Ruminants Hair/Wool in District Tharparkar, Sindh

Occurrence of Anthrax Spores in Small Ruminants Hair/Wool in District Tharparkar, Sindh

Prevalence of bovine tuberculosis in buffaloes in Hyderabad and Tando Allahyar districts of Sindh, Pakistan

Bacterial Contamination of Raw Meat and Butchers Equipment in Retail Shops in Tando-Allahyar, Pakistan

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