Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil

Abstract

The presence of anti leptospiral agglutinins (microscopic agglutination test -MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction -PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inϐlammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue. RESUMO.-[Sorologia, reação em cadeia da polimerase e histopatologia para leptospirose em amostras coletadas de vacas leiteiras, em matadouro da região de Parnaíba, Piauí.] Foi investigada a presença de aglutininas anti leptospiras (reação de soroaglutinação microscópica -SAM), de DNA de leptospiras no rim e na urina (reação de cadeia pela polimerase -PCR), bem como de lesões no rim, pulmão, ϐígado, útero, ovário e placenta (histopatologia e imunohistoquímica), em materiais colhidos, por ocasião do abate, de bovinos originários da bacia leiteira da região de Parnaíba, Piauí, Brasil. Na SAM o sorovar predominante foi o Hardjo com maior número de animais reagentes para a estirpe Hardjobovis/Sponselee. Antígenos anti leptospira foram marcados em 20 animais positivos nas células epiteliais e do endotélio vascular, endotélio dos capilares glomerulares e na cápsula de Bowman, somente nos animais infectados. O inϐiltrado inϐlamatório foi maior no rim do que nos demais órgãos. A PCR foi positiva em amostras de urina e tecido renal. TERMOS DE INDEXAÇÃO: Leptospirose, imuno-histoquímica, PCR, bovino.

Seroepidemiological pattern of leptospirosis in bovine of South Gujarat, India

“…Mandal et al [13] reported 16.84%, and Savalia and Mahendra [14] reported 14.77% seroprevalence of leptospirosis in West Bengal and Gujarat, respectively, which compare well with our present findings. Among different countries low to high seroprevalence rate has been recorded like 3.4% [15] in Turkey, 37.75% [16], 22.5% [17], 21.05% [18] in Iran, 27.7% [19] in Malaysian, 38.3% [20] and 50.71% [21] in Brazil.…”

Evaluation of ‘white-spotted kidneys’ associated with leptospirosis by polymerase chain reaction based <i>LipL32</i> gene in slaughtered cows

“…Infected cattle may not show any clinical signs of disease, but excrete the organisms in their urine. Cattle therefore play an important role in spreading the infection to other susceptible animals and to human populations at risk, such as farmers and veterinarians (Levett 2001;Mineiro et al 2011). The aim of this study was to determine the correlation between white-spotted kidneys with leptospirosis in cattle at an abattoir, using the polymerase chain reaction (PCR) technique.…”

Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions

“…Moreover, in some of the countries also serovar hardjo was found to be most prevalent i.e. in Arizona, USA (Songer et al, 1983), Malaysia (El Jalii, 2008, Turkish (Kocabiyik andCetin, 2004), Iraq (Al-Badrawi et al, 2010) and Brazil (Mineiro et al, 2011). Besides being an important cause of bovine abortion, reduced fertility and agalactia, serovar Hardjo also poses a potential zoonotic threat to humans who are exposed to infected cattle (Balmurugan et al, 2016).…”