Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions

Abstract

A zoonotic disease Leptospirosis is caused by pathogen of the genus Leptospira and it is an emerging global public health problem. In the present study a total of 266 cattle blood and sera samples were collected from the towns and villages of Nagpur, Wardha, Bhandara Gadchiroli and Durg districts during December 2017 to 2018 and tested for the presence of Leptospira. These samples were from randomly selected herds with history of repeated breeding, abortion, reproductive disorders, etc. also including some apparently healthy animals. The presence of leptospiral DNA in blood sample was assayed by PCR amplification of rrs (16S rRNA) gene. Antibodies against Leptospira serovars were tested using an enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT). Out of 266 blood samples, in 33 samples leptospires DNA was identified with the frequency of 12.40%. A total of 53 cattle sera were positive in commercial Leptospira Bovine Hardjo ELISA kit indicated 19.92% seroprevalence. In the MAT analysis 120 samples revealed presence of different serovars with the seropositivity 45.11%. The study supports the probable role of cattle in maintaining Leptospira Hardjo along with some other serovars and warrants an intensive control and surveillance programme for reducing leptospirosis in cattle.

Seroprevalence and Associated Risk Factors of Leptospira interrogans Serogroup Sejroe Serovar Hardjo in Dairy Farms in and around Jimma Town, Southwestern Ethiopia

“…This result was in agreement with the findings of Odontsetseg et al [ 20 ] (23.50%) in Mongolia, Schoonman and Swai [ 21 ] (30.30%) in Tanzania, Gamage et al [ 22 ] (20.30%) in Sri Lanka and Subharat et al [ 23 ] (27.4%) in Australia. Similarly, the present finding was in congruence with results of previous studies by Taddei et al [ 24 ] (19.30%) in unvaccinated animals of Colombian dairy farm, Ismail et al [ 25 ] (26.25%) in Jordan, Tabatabaeizadeh et al [ 26 ] (19.10%) in Iran, Ngbede et al [ 27 ] (25% and 23.90%) in different dairy farms of Zaria (Nigeria), Balamurugan et al [ 28 ] (23.68%) in Chhattisgarh of India, Ismail et al [ 25 ] (28.75%) in Jordan and Shilpa et al [ 29 ] (19.92%) in Nagpur of Indian dairy farms.…”

Seroprevalence of leptospirosis among slaughtered cattle in Thrissur, Kerala#

“…In Alappuzha district of Kerala, Sreekutty et al (2020) reported that the predominant serovars obtained in cattle were Grippotyphosa, Sejroe and Autumnalis. The predominant serovars detected in cattle were serovar Pomona, Hebdomadis, Javanica, Icterohaemorrhagiae and Sejroe by Moon et al (2019), in Nagpur. Soman (2004) reported that the predominant serovars among cattle in Thrissur district, Kerala were Sejroe, Potac and Pomona.…”