Aim: Seroepidemiological study of leptospirosis in cattle of various South Gujarat district (Navsari, Tapi, Surat, Valsad). Materials and Methods:Whole blood samples were collected randomly from different age groups, and breeds of cattle of either sex reared in different districts (Navsari, Surat, Tapi, Valsad) of South Gujarat. To obtain serum, whole blood was kept in slanting position in 9.0 ml plain vacutainers until serum extracted out of the whole blood. Then these 9.0 ml plain vacutainers were centrifuged at 7000 rpm for 10 min. The straw colored serum was then collected in 1.5 ml sterile cryo vials and aliquoted and stored at −20°C for microscopic agglutination test. Results:In the present study, overall 12.81% (51/398) seroprevalence were recorded with highest seroprevalence (47.06%, 24/51) from Valsad followed by Navsari (9.14%, 18/197), Surat (6.90%, 2/29) and Tapi (5.79%, 7/121) among cattle. The seroprevalence rate of breed and sex wise did not differ significantly (p≤0.05). Maximum incidence of seropositivity was found above 4 years (16.32%, 39/239) of age group followed by animals between 1 and 4 years (9.68%, 12/124). Thus, the age was significantly influencing the seropositivity (p≤0.05). In cattle out of 398 sera screened, 51 were positive with one or more serovars. The highest seropositivity was recorded against serovar Pomona (28.89%).Conclusions: Overall 12.81% seroprevalence of leptospirosis in apparently healthy and clinically ailing bovine of South Gujarat indicating potential zoonotic risk to farmers, labor, and animal owners.

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Aim:The present study was designed to evaluate clinicopathological alterations in naturally occurring leptospirosis in goats of South Gujarat region, Gujarat.Materials and Methods:A total 459 blood/serum and 292 urine samples were collected from different districts of South Gujarat region, India. Blood/serum and urine samples were subjected to hematobiochemical analyses and urinalyses. The serum samples were screened for anti-leptospiral antibodies using the microscopic agglutination test (MAT). On the bases of presence or absence of anti-leptospiral antibodies in serum, seropositive and seronegative groups were made. The results were analyzed using standard statistical methods to know pathological changes in the disease.Results:In MAT, out of 459, 116 goats were seropositive, and 343 were seronegative. In hematobiochemical analyses, statistically significant (p<0.01) decrease in values of packed cell volume, hemoglobin (Hb) concentration, mean corpuscular Hb concentration and total protein and increased activity/level of alanine aminotransferase, aspartate aminotransferase and total bilirubin between seropositive and seronegative goats were noted. Urinalyses did not reveal any specific changes. In the dark field microscopy, urine samples were found to be negative for leptospires.Conclusion:Hematobiochemical changes noted in seropositive goats were indicative of hepatic damage, and this knowledge would aid in the therapeutic management of the disease.

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