Polymerase Chain Reaction in the Diagnosis of  Spontaneous Leptospirosis in Bovines

Patel, Jignesh M.; Vihol, Priti D.; Prasad, M. C.; Patel, Jayshree; Raval, J. K.; Kalyani, I. H.

doi:10.20546/ijcmas.2017.612.195

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…PCR has the advantage that it does not require the isolation of the organism and detects DNA from both viable and nonviable organisms (Noubade et al, 2002). The finding of the study supported the findings of Cheema et al, (2007), Jafari et al, (2011 and Patel et al, (2017) who too carried out the investigation on field samples.…”

Section: Resultssupporting

confidence: 90%

Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions

Moon¹,

Chaudhari²,

Zade³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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A zoonotic disease Leptospirosis is caused by pathogen of the genus Leptospira and it is an emerging global public health problem. In the present study a total of 266 cattle blood and sera samples were collected from the towns and villages of Nagpur, Wardha, Bhandara Gadchiroli and Durg districts during December 2017 to 2018 and tested for the presence of Leptospira. These samples were from randomly selected herds with history of repeated breeding, abortion, reproductive disorders, etc. also including some apparently healthy animals. The presence of leptospiral DNA in blood sample was assayed by PCR amplification of rrs (16S rRNA) gene. Antibodies against Leptospira serovars were tested using an enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT). Out of 266 blood samples, in 33 samples leptospires DNA was identified with the frequency of 12.40%. A total of 53 cattle sera were positive in commercial Leptospira Bovine Hardjo ELISA kit indicated 19.92% seroprevalence. In the MAT analysis 120 samples revealed presence of different serovars with the seropositivity 45.11%. The study supports the probable role of cattle in maintaining Leptospira Hardjo along with some other serovars and warrants an intensive control and surveillance programme for reducing leptospirosis in cattle.

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Section: Resultssupporting

confidence: 90%

Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions

Moon¹,

Chaudhari²,

Zade³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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“…Human and domestic animals like cattle, buffalo, goat and sheep are infected frequently over the year. Incidence and prevalence studies are reported earlier in this region from human and domestic animals [17][18][19][20][21][22][23][24][25]. However, prevalence was not studied from dogs in Gujarat.…”

Section: Introductionmentioning

confidence: 99%

Detection and Prevalence of Canine Leptospirosis from Navsari District of South Gujarat, India

Desai

Makwana

Solanki

et al. 2020

MRJI

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Aims: Leptospirosis is endemically prevalent in coastal area of south Gujarat. Generally, pets are vaccinated against Leptospira interrogans but whosoever left unvaccinated, are prone to infection. The present study was conducted to detect canine leptospirosis and its prevalence from south Gujarat. Place and Duration of Study: A total of 46 serum samples and 33 urine samples were collected from 56 dogs suspected of having leptospira infection. The study was done from September 2019 to May 2020. Methodology: Serum samples were diagnosed by IgG based ImmunoComb solid phase dot ELISA test for antibody against genus specific Leptospira interrogans. Urine samples were visualized under dark field microscopy (DFM) following laboratory manual on leptospirosis of WHO. Sex and age of dogs were noted to find out the percent positivity and prevalence. Chi square test was applied to find out any significant difference among the age groups. Results: Out of 46 serum samples, 17 (36.95%) found positive by dot ELISA and out of 33 urine sample, 13 (39.39%) found positive by DFM. Sex wise prevalence found 42.10 percent (16/38) in Male while 55.55 percent (10/18) in female dogs. Age group wise prevalence was recorded 63.63 percent (07/11) in 1- ≤ 3 years group. 27.77 per cent (05/18) in > 3- ≤ 6 years group whereas, 51.85 percent (14/27) in > 6- ≤ 12 years group. Conclusion: Detection and prevalence of canine leptospirosis were studied first time from Gujarat. Overall 46.42 percent (26/56) dogs were diagnosed positive for canine leptospirosis. Dogs having age group of 1- ≤ 3 years found more susceptible. Prevalence was found more in female than male dogs.

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“…Leptospirosis is one of the major causes of abortion, still births, infertility, repeat breeding, failure to thrive, decreased milk production, mastitis in ruminants and great economic loss along with zoonotic infections globally (Costa et al, 2015 andBalamurugan et al,2016). In India, the prevalence of Leptospirosis in farm animals including buffaloes in many states has been reported earlier (Varma et al, 2001;Sharma et al, 2003;Sivaseelan et al, 2003;Balakrishnan et al, 2011 andPatel et al, 2017). The endemicity of Leptospira in India since has been reported in 20 th century (Chowdry et al, 1903, Wooly et al, 1911.…”

Section: Introductionmentioning

confidence: 99%

Study of Leptospira Infection in Buffaloes through Molecular and Bacteriological Techniques

Dushyant

Wiqar

Sandeep

et al. 2020

IJAR

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In India buffaloes are reared for milk, meat and draft purposes. The economic importance of buffalo rearing in Nagpur is more than cow and contribute major share to the milk production of the district. Apart from its zoonotic implications; Leptospirosis is one of the major causes of abortion, still births, infertility, repeat breeding, decreased milk production, mastitis in ruminants. Considering its endemic nature in India, the present study is aimed at estimating the prevalence of leptospirosis in buffaloes and to establish the possible route of transmission through related canine, rodent and environment. A total of 621(299 each blood and sera and 23 urine) samples collected from 196 apparently healthy and 103 clinically suspected buffaloes and blood samples of 21 stray dogs along with 27 environmental samples were subjected to PCR targeting sec Y gene (202bp) and 16SrRNA gene (331bp).A total of 92 samples including 53 blood samples (buffalo= 45, rodent= 5, canine= 3), 30 buffalo sera samples, four urine samples (buffalo=2 and canine=2) and five environmental samples comprising of 3 water and 2 feed, were processed for isolation of Leptospira in the EMJH medium. Out of 196 blood samples of apparently healthy buffaloes a total of 27 (13.77%) animals were found positive for 16S rRNA gene and 3(1.53%) for sec Y gene. Similarly, 17.47% (18/103) animals were positive for 16S rRNA gene while 7(6.79%) for sec Y gene. A total of 30 (15.30%) apparently healthy animals were positive for either 16SrRNA gene or secY gene. Whereas 25(24.27%) samples from suspected animals were positive for either of the gene. All the urine samples from buffaloes, dogs and environmental samples were negative for presence of both the genes. All the samples processed for isolation of organism turned negative. The accurate and rapid diagnosis of leptospirosis is important and beneficial for surveillance and investigation of transmission dynamics of the disease. Even though the active infection can be readily diagnosed through blood samples it is essential to screen each sample by various tests for proper understandings about the disease distribution.

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Polymerase Chain Reaction in the Diagnosis of Spontaneous Leptospirosis in Bovines

Cited by 5 publications

References 7 publications

Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions

Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions

Detection and Prevalence of Canine Leptospirosis from Navsari District of South Gujarat, India

Study of Leptospira Infection in Buffaloes through Molecular and Bacteriological Techniques

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