Serological trail of Brucella infection in an urban slum population in Brazil

Ángel, Martha Olivera; Ristow, Paula; Ko, Albert I.; Dilorenzo, Cecilia

doi:10.3855/jidc.2347

Cited by 19 publications

(17 citation statements)

References 20 publications

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“…Symptomatic human infections are rare, probably because of the low virulence of B. canis (Sayan et al, ). In most cases, canine Brucella is the only principal pathogen of dogs, but Brucella is not pathogenic to the healthy human population or is quickly recognized and cleared by the immune system after infection (Angel, Ristow, Ko, & Di‐Lorenzo, ). The results of previous studies suggest that healthy humans might be moderately resistant to clinical illness caused by B. canis infection (Hensel et al, ); However, children and immunosuppressed persons might be at higher risk for acquiring the disease (Lucero, Maldonado, et al, ; Marzetti, Carranza, Roncallo, Escobar, & Lucero, ).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Liu

Wang

et al. 2019

Transbound Emerg Dis

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Brucellosis is a global pandemic infectious zoonosis. Brucella canis is a rare source of human brucellosis in China, and its public health significance remains under debate. Moreover, data pertaining to the epizootiological characteristics and geographical origin of B. canis on a nationwide scale are limited, and the risk to public safety posed by B. canis infections is unknown. The MLVA (multilocus variable‐number tandem repeat analysis) assay can be helpful to analyse epidemiological correlations among Brucella isolates and to track their geographic origins. To accomplish this task, MLVA‐16 was used to analyse the epidemiological links of 63 isolates obtained from dogs and humans. Sixty‐three B. canis strains were sorted into three large clusters (A, B and C) and 50 different genotypes (GT1–50), and 43 unique genotypes were represented by single isolates, suggesting that these strains had no obvious epidemiological links and that canine brucellosis is predominantly sporadic in China. The other seven shared genotypes (among a total of 20 isolates) were each represented by two to eight isolates, indicating that strains from each shared genotype were epidemiologically correlated. Five of the shared genotypes were from 16 strains obtained from Beijing, indicating that canine brucellosis in Beijing originates from multipoint outbreaks with multiple sources of infection. Based on comprehensive case analysis of clinical B. canis infection, we preliminarily suggest that human B. canis infections are associated with Mycoplasma pneumoniae infection that results in decreased patient immunity. B. canis may have limited epidemiological significance for the healthy population, but it remains a significant threat to the canine breeding industry and to humans who come into close contact with dogs. Based on MLVA‐11 data, B. canis strains were clustered into 16 genotypes and divided into five evolutionary branches; these data confirm that this population covers an extensive geographic area and exhibits characteristics of the origin and evolution of co‐existing introduced and locally native lineages. We believe this study will contribute to strengthening efforts to prevent and control canine brucellosis and to improve public understanding of the health risks posed by B. canis.

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Section: Discussionmentioning

confidence: 99%

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Liu

Wang

et al. 2019

Transbound Emerg Dis

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“…This strategy involves screening protein arrays using sera from vaccinated or infected animals to identify target vaccine antigens. Sera from infected or convalescent patients have also been used to screen protein arrays containing pathogen proteins to characterize rates of infection and identify bacterial antigens [ 44 ]. Following identification of loci of interest, targeted gene mutations can be introduced at these loci using conventional bacterial gene targeting approaches [ 85 ] or gene editing technology [ 106 ].…”

Section: Early Development and Use Of Lavsmentioning

confidence: 99%

The Case for Live Attenuated Vaccines against the Neglected Zoonotic Diseases Brucellosis and Bovine Tuberculosis

et al. 2016

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Vaccination of humans and animals with live attenuated organisms has proven to be an effective means of combatting some important infectious diseases. In fact, the 20th century witnessed tremendous improvements in human and animal health worldwide as a consequence of large-scale vaccination programs with live attenuated vaccines (LAVs). Here, we use the neglected zoonotic diseases brucellosis and bovine tuberculosis (BTb) caused by Brucella spp. and Mycobacterium bovis (M. bovis), respectively, as comparative models to outline the merits of LAV platforms with emphasis on molecular strategies that have been pursued to generate LAVs with enhanced vaccine safety and efficacy profiles. Finally, we discuss the prospects of LAV platforms in the fight against brucellosis and BTb and outline new avenues for future research towards developing effective vaccines using LAV platforms.

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“…Canine brucellosis is considered the main cause of reproductive failures in dogs [ 4 ], provoking late abortions in females and epididymitis and/or prostatitis in male dogs, leading to infertility, and non-reproductive symptoms such as ophthalmitis, diskospondylitis, lymphadenopathy, and splenomegaly [ 1 , 2 , 5 ]. Both, infected dogs and healthy dogs harbouring B. canis have the potential to transmit the bacteria to humans, mainly by oronasal contact, occupational interaction with infected animals, or laboratory accidents [ 6 – 9 ]. When humans are infected, B. canis frequently induce mild or asymptomatic infections that may remain undiagnosed for protracted periods [ 3 , 10 – 12 ] and clinical signs include headache, recurrent fever, weakness, fatigue, chills, sweats, and weight loss [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Variability in the response of canine and human dendritic cells stimulated with Brucella canis

Pujol

Castillo

Álvarez

et al. 2017

Vet Res

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Brucella canis is a small intracellular Gram-negative bacterium whose primary host is the dog, but it also can cause mild human brucellosis. One of the main causes of an inefficient immune response against other species of Brucella is their interaction with dendritic cells (DCs), which affects antigen presentation and impairs the development of an effective Th1 immune response. This study analysed the cytokine pattern production, by RT-qPCR and ELISA, in human and canine DCs against whole B. canis or its purified LPS. Human and canine DCs produced different patterns of cytokines after stimulation with B. canis. In particular, while human DCs produced a Th1-pattern of cytokines (IL-1β, IL-12, and TNF-α), canine cells produced both Th1 and Th17-related cytokines (IL-6, IL-12, IL-17, and IFN-γ). Thus, differences in susceptibility and pathogenicity between these two hosts could be explained, at least partly, by the distinct cytokine patterns observed in this study, where we propose that human DCs induce an effective Th1 immune response to control the infection, while canine DCs lead to a less effective immune response, with the activation of Th17-related response ineffective to control the B. canis infection.Electronic supplementary materialThe online version of this article (10.1186/s13567-017-0476-8) contains supplementary material, which is available to authorized users.

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Serological trail of Brucella infection in an urban slum population in Brazil

Cited by 19 publications

References 20 publications

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

The Case for Live Attenuated Vaccines against the Neglected Zoonotic Diseases Brucellosis and Bovine Tuberculosis

Variability in the response of canine and human dendritic cells stimulated with Brucella canis

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