Serological and Molecular Phylogenetic Detection of Coxiella burnetii in Lactating Cows, Iraq

Gharban, Hasanain A. J.; Yousif, Afaf Abdulrahman

doi:10.30539/ijvm.v44i(e0).1020

Cited by 10 publications

(9 citation statements)

References 47 publications

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“…The quarantine measures could influence these variations, type, and sensitivity of diagnostic methods, type of studied sample, age and sex of animals, poor hygienic and sanitary conditions. The current study reveals significant variations between the seropositive rate 19.8% of ELISA and PCR 4.4%, this shows that only a fraction of exposed animal carries the pathogen at a given time might be due to continuous immunological responses of the infected host, which leads to decrease and/or interrupted shedding of this pathogen (5).…”

Section: Discussionmentioning

confidence: 62%

“…C. burnetii is an occupational pathogen of farmers, slaughterhouse workers, and veterinarians and can be transmitted by inhaling aerosolized contaminated dust, direct contact with infected tissues and fluids, as well as by arthropod bites (3). Multiple hosts can serve as the main reservoir for infection, particularly the infected females that shed vast numbers of highly stable bacteria into their birth products (amniotic fluids and placenta) and smaller amounts in milk, feces, and urine which may continue over several months (4)(5)(6). Worldwide, numerous studies investigate the prevalence of Coxiella in camels such as the United Arab Emirates (7), Egypt (8), Algeria (9), Saudi Arabia (10), and Iran (11).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular assays are beneficial and safe methods with high specificity and sensitivity and decrease the time for detecting targeted C. burnetii DNA in suspected samples (4). Serological assays and direct detection by PCR must be used as combinatory methods to diagnose infection (5). In Iraq, the dromedary camel (Camelusdromedarius) is one of the most important domesticated animals in arid and semi-arid areas.…”

Section: Introductionmentioning

confidence: 99%

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First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq

Al-Graibawi¹,

Yousif²,

Gharban³

et al. 2021

IJVS

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This study aims to detect Coxiella burnetii in one-humped female camels (Camelus dromedarius) using ELISA and confirmation of infection by PCR with the phylogenetic analysis of local isolates. The 91 adult female camels were selected for clinical examination and blood sampling from different areas in Badra and Al-Numaniyah districts in Wasit governorate, Iraq, from February to April 2019. The prevalence of Coxiella (C.) burnetii was 19.8% and 4.4% by ELISA and PCR, respectively. Targeting 16S rRNA genes from three positive samples were documented in the Genbank-NCBI under accession numbers of MN900579.1, MN900580.1, and MN900581.1. Clinical evaluation revealed insignificant variation in temperature, pulse, respiratory rates, and lymph node enlargement among the positive and negative animals. The findings also showed that camels of the Badra regions have positive signs. burnetii compared to other regions, and the infection was increased significantly in April and March. In conclusion, our findings confirmed the prevalence of C. burneth among Iraqi female camels, suggesting that these animals might be a source of the pathogen for humans and other animal species. Therefore, further studies are necessary to provide more detailed data about the prevalence of C. burnetiito to improve effective control measures.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq

Al-Graibawi¹,

Yousif²,

Gharban³

et al. 2021

IJVS

Self Cite

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“…was present in less percentage like Dematiaceous spp. (2.5%) it is a common soil saprophyte and an important pathogen; and has ability to surface adherence and produce protease and collagenases, and microorganism causes infection which can be locally invasive lung or disseminated to other organ (30,31,32). Cryptococcus neoformans colonies on Bird Seed Agar produces brown colored melanin pigment due to its produces enzyme phenoloxidase, which necessary in melanin synthesis and causing the formation of brown pigmented colonies (33) which considered as a positive result for melanin production and this result agreement with (3,14).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of cryptococcus neoformans from local cats in Baghdad province

Al-Bakir

Abd-Alshaheed

2022

ijhs

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This study provides a brief review of approaches fordetection of fungi that cause pulmonary infection in cats. The 40 samples was taken randomlyfromlocal Iraqi cats in Baghdad provinceto detection of fungal infection that cause respiratory disease in cats, especially Cryptococcus neoformans that considered one of the most important pathogens cause respiratory symptoms in cats, the studied and isolated during the period of October 2021 to march 2022. Samples were taken from infected cats in veterinary clinic in Baghdad province; all samples were cultured on Saboroud Dextrose Agar, Bird Seed Agar and Cryptococcus Differential Agar for Cryptococcus spp. isolation and identification. The results showed that 40 swaps taken from the pharynx of infected cats, included: Cryptococcus neoformans 12 (30.0%), Aspergillus fumigatus 2 (5.0%), Penicillium spp. 6 (15.0%), Aspergillus niger 3 (7.5%), Dimatiaceous spp. 1 (2.5%), Fusarium spp. 1 (2.5%), Rhizopus spp. 2 (5.0%), Candida spp. 3 (7.5%), Aspergillus spp. 10 (25.0%)and the presence of infection in female (60%) more than male (40%). These findings indicate that there may be a risk for animal exposure to pulmonary fungal disease due to inhalation of fungal spores, contaminated environment, hormonal disturbance in female, viral infection that cause of immune deficiency.

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“…Many researchers have focus in their scientific work on the local mechanisms action of tramadol HCL, it is atypical opioid, but its local mechanisms of action have not yet been fully understood (16)(17)(18). Many studies refer to the local anesthetic effects of tramadol HCL as follows: anesthetic and opioid /analgesic (19)(20)(21). Recent studies have been shown the possible local mechanisms action of tramadol by favoring the opening of nonspecific voltage-dependent potassium (K + ) channels (22), acting in the nitric oxide pathway (23), and the a agonistic action on vanilloid receptor 1 (TRPV-1 transient receptor potential vanilloid-1) that apart from local analgesic effectcan exert undesired local side effects such as burning pain and erythema, with high concentration of tramadol blockade of the Nmethyl-D-aspartate receptors, local anesthetic effect of tramadol can be explained by direct blocking of voltage-dependent sodium (Na + ) channels (24)(25)(26).…”

Section: Introductionmentioning

confidence: 99%

Tramadol as local anesthetic drug for maxillary sinuses trephination in equine species

Sahep

Omar

Nazhat

2022

ijhs

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This project was designed to study the incidence, onset, duration and potency of tramadol, in donkeys can be evaluated as local anesthetic drug by infiltration in maxillary sinus for trephination. In this study, five adult donkeys of both sexes of local breed were used for induce trephination of maxillary sinus that was given 2% xylazine hydrochloride intravenously followed by local infiltration of 5% tramadol with aseptic technique. Parameters were used for evaluation, physical and clinical examination which includes: degree of chewing, degree of pain during palpation, measuring of anesthetic time, secretion of lacrimal gland was measured after induction of anesthetic drug locally. The results demonstrated that there are no any significant changes in heart rate, respiratory rate and rectal body temperature .While the anesthetic parameters showed no any significant changes about pain during palpation and absent of chewing movement with secretion of lacrimal gland was seen. Increase in anesthesia duration time from 30-45 minute and with the potency and recovery was excellent after surgical operation.

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Serological and Molecular Phylogenetic Detection of Coxiella burnetii in Lactating Cows, Iraq

Cited by 10 publications

References 47 publications

First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq

First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq

Isolation and identification of cryptococcus neoformans from local cats in Baghdad province

Tramadol as local anesthetic drug for maxillary sinuses trephination in equine species

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