Serologic Survey for Agglutinins to Brucella Canis in Florida Residents *

Hoff, Gerald L.; Schneider, Nathan J.

doi:10.4269/ajtmh.1975.24.157

Cited by 12 publications

(7 citation statements)

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“…4 % positive reactions were detected (Lewis & Anderson, 1973); 0 . 59 % in Florida residents (Hoff & Nichols, 1974;Hoff & Schneider, 1975) and 67 . 8 % in Oklahoma (Monroe et al, 1975); an investigation in Germany found antibody titres in 6 out of 1915 sera (Carmichael et al, 1980).…”

Section: Resultsmentioning

confidence: 99%

“…Human infections are probably more common than indicated in published reports, though serological methods and criteria for evaluating results vary greatly. The serological techniques most often used to detect B. canis antibodies in humans are the agglutination tests (Lewis & Anderson, 1973;Hoff & Schneider, 1975;Hoff & Nichols, 1974;Monroe et al, 1975;Flores-Castro & Segura, 1976;Ying et al, 1999;Polt & Schaefer, 1982). The infection was diagnosed by serological methods in a 17-month-old child, a woman with fever of unknown origin and a man with granulomatous hepatitis and splenomegaly (Tosi & Nelson, 1982;Rousseau, 1985;Schoenemann et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Diagnosis of human brucellosis caused by Brucella canis

Lucero

Escobar

Ayala

et al. 2005

Journal of Medical Microbiology

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The transmission of Brucella canis to man commonly occurs through contact with infected dogs or their secretions, or through direct laboratory exposure. The disease is underdiagnosed due to a general lack of serological testing facilities and misconceptions concerning its prevalence. This report shows the potential use of an indirect ELISA (IELISA) for the diagnosis of human brucellosis caused by B. canis in a population of patients negative by smooth-Brucella antigen tests but positive by rapid slide agglutination test (RSAT). One hundred and ten sera from asymptomatic people found negative by tests using smooth Brucella abortus antigen and by RSAT showed an IELISA specificity of 100 % when a cut-off value of 27 % positivity (%P) was selected. For 17 sera from patients with positive B. canis culture or in close contact with culture-positive dogs, the IELISA sensitivity was 100 % with the same cut-off value. The positive patients presented clinical symptoms similar to brucellosis caused by other species of Brucella and some of them received antibiotic treatment and made good progress. Using this cut-off value, we studied 35 patients with negative blood cultures but positive RSATs, and IELISA detected 18 as positive; of the 17 IELISA-negative, two were RSATpositive at dilution 1 : 2 and 15 were weakly positive with pure serum. These samples were probably from patients at an early stage of infection or indicate false-positive results. No cross-reaction was observed among the sera from nine cases with a diagnosis other than brucellosis, but crossreactivity was evident in sera from patients infected with smooth-Brucella species. Since routine brucellosis diagnosis does not include B. canis investigation, infection with this species may be more widespread than is currently suspected. The RSAT could be a suitable screening test for the diagnosis of B. canis human brucellosis, and a supplementary technique, such as IELISA, performed on all positive RSAT samples that were negative by B. abortus antigen could ensure diagnostic specificity and confirm the diagnosis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diagnosis of human brucellosis caused by Brucella canis

Lucero

Escobar

Ayala

et al. 2005

Journal of Medical Microbiology

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“…Moreover, very few studies have been conducted in humans worldwide: in the United States, in 1973 researchers found 0.4% of military recruits were positive [25]. Two additional studies that were also conducted in 1975 involving people in close contact with dogs [26] indicated low positivity, while in the State of Florida 3 positives out of 303 individuals in the general population [27] were detected. Recently global reports about B. canis infections in humans have become more frequent.…”

Section: Resultsmentioning

confidence: 99%

Serological trail of Brucella infection in an urban slum population in Brazil

Ángel

Ristow

et al. 2012

J Infect Dev Ctries

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Introduction Brucellosis is a re-emerging zoonosis with new cases reported each year in many Latin American countries, but it is mostly under-recognized. This study presents a serological investigation of infection with Brucella abortus and Brucella canis in a poor urban community in the city of Salvador, Brazil. Methodology Human sera (n = 180) were randomly selected from 3,171 samples taken from healthy individuals during 2003-2004 and tested with C-ELISA for B. abortus and I-ELISA for B. canis. Results Thirteen percent (24/180) of the individuals were positive for B. abortus and 4.6 % (8/174) were positive for B. canis. Among the variables studied only age (older than 45 years) appeared to be a risk factor for the detection of Brucella antibodies. Conclusion These results indicate the presence of Brucella infection in this settlement and highlight the need to understand the epidemiology of infection under these circumstances to establish the necessary measures for surveillance and control.

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“…The perceived infrequency of human infection with B. canis and the lack of reliable diagnostic tools for disease detection has led to few serologic surveys in humans. Our current understanding of prevalence of B. canis infection in humans comes from a handful of serologic surveys that use diagnostic tests available for dogs and thus may not be truly representative ( 3 , 38 , 42 – 44 ).…”

Section: Public Health Implicationsmentioning

confidence: 99%

“…In the United States, cross-sectional serologic surveys of military recruits and Florida residents and case–control surveys of animal caretakers with occupational exposure to canids documented an extremely low B. canis seropositivity (0.4%–0.6%) ( 38 , 42 , 44 ). Veterinarians from Florida with occupational exposure to dogs were also surveyed but were all negative according to serologic testing ( 38 ).…”

Section: Public Health Implicationsmentioning

confidence: 99%

Brucellosis in Dogs and Public Health Risk

Hensel

Negrón²,

Arenas-Gamboa

2018

Emerg. Infect. Dis.

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Brucella canis infects dogs and humans. In dogs, it can cause reproductive failure; in humans, it can cause fever, chills, malaise, peripheral lymphadenomegaly, and splenomegaly. B. canis infection in dogs is underrecognized. After evaluating serologic data, transmission patterns, and regulations in the context of brucellosis in dogs as an underrecognized zoonosis, we concluded that brucellosis in dogs remains endemic to many parts of the world and will probably remain a threat to human health and animal welfare unless stronger intervention measures are implemented. A first step for limiting disease spread would be implementation of mandatory testing of dogs before interstate or international movement.

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Serologic Survey for Agglutinins to Brucella Canis in Florida Residents *

Cited by 12 publications

References 0 publications

Diagnosis of human brucellosis caused by Brucella canis

Diagnosis of human brucellosis caused by Brucella canis

Serological trail of Brucella infection in an urban slum population in Brazil

Brucellosis in Dogs and Public Health Risk

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