Serodiagnosis of Lyme borreliosis infection using surface plasmon resonance

Nagel, Thomas; Gajovic‐Eichelmann, Nenad; Tobisch, Steffen; Schulte-Spechtel, Ulrike; Bier, Frank F.

doi:10.1016/j.cca.2008.04.009

Cited by 16 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A piezoelectric assay was described by Campbell and Mutharasan [167] for the detection of between 100 and 10,000 oocysts/mL of Cryptosporidium parvum , while Kang and co-workers [168] developed a Biacore-based immunosensor assay which allowed the detection of between 1 × 10 2 – 1 × 10 6 oocysts/mL. The flexibility of using this methodology has also been illustrated by the ability to also detect other parasitic pathogens, including Schistosoma japonicum [169-172] and Borrelia burgdorferi [173], which act as causative agents of schistosomaisis and lyme borreliosis, respectively. These assays use amperometric [169-171], piezoelectric [172] and optical [173] -based platforms.…”

Section: Immunosensor Assays For the Detection Of Viral Pathogens Mamentioning

confidence: 99%

“…The flexibility of using this methodology has also been illustrated by the ability to also detect other parasitic pathogens, including Schistosoma japonicum [169-172] and Borrelia burgdorferi [173], which act as causative agents of schistosomaisis and lyme borreliosis, respectively. These assays use amperometric [169-171], piezoelectric [172] and optical [173] -based platforms.…”

Section: Immunosensor Assays For the Detection Of Viral Pathogens Mamentioning

confidence: 99%

See 1 more Smart Citation

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Byrne

Stack

Gilmartin

et al. 2009

Sensors

323

193

View full text Add to dashboard Cite

Antibody-based sensors permit the rapid and sensitive analysis of a range of pathogens and associated toxins. A critical assessment of the implementation of such formats is provided, with reference to their principles, problems and potential for ‘on-site’ analysis. Particular emphasis is placed on the detection of foodborne bacterial pathogens, such as Escherichia coli and Listeria monocytogenes, and additional examples relating to the monitoring of fungal pathogens, viruses, mycotoxins, marine toxins and parasites are also provided.

show abstract

Section: Immunosensor Assays For the Detection Of Viral Pathogens Mamentioning

confidence: 99%

Section: Immunosensor Assays For the Detection Of Viral Pathogens Mamentioning

confidence: 99%

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Byrne

Stack

Gilmartin

et al. 2009

Sensors

323

193

View full text Add to dashboard Cite

show abstract

“…Surface plasmon resonance (SPR) biosensors have made great strides both in terms of technology and applications [16] such as characterizing and quantifying biomolecular interactions [17][18][19], determination of affinity and binding constants [20][21][22], monitoring [23,24], diagnosis [25][26][27][28] and DNA sensing [29,30].…”

Section: Introductionmentioning

confidence: 99%

4-Aminophenyl boronic acid modified gold platforms for influenza diagnosis

Di̇ltemi̇z

Ersöz

Hür

et al. 2013

Materials Science and Engineering: C

View full text Add to dashboard Cite

“…Nagel et al restricted their SPR studies for serological detection of Lyme borrelioses to two widely used antigens. The whole proteins as well as two peptides, representing immunodominant domains, were used as capture probes [61]. But in general, different flow chambers have different antigens, and a direct comparison of kinetics and affinity ranking of more than four interactions is not practicable.…”

Section: A Cross Platform Comparisonmentioning

confidence: 99%

Validation Processes of Protein Biomarkers in Serum—A Cross Platform Comparison

Köhler

Seitz

2012

Sensors

View full text Add to dashboard Cite

Due to insufficient biomarker validation and poor performances in diagnostic assays, the candidate biomarker verification process has to be improved. Multi-analyte immunoassays are the tool of choice for the identification and detailed validation of protein biomarkers in serum. The process of identification and validation of serum biomarkers, as well as their implementation in diagnostic routine requires an application of independent immunoassay platforms with the possibility of high-throughput. This review will focus on three main multi-analyte immunoassay platforms: planar microarrays, multiplex bead systems and, array-based surface plasmon resonance (SPR) chips. Recent developments of each platform will be discussed for application in clinical proteomics, principles, detection methods, and performance strength. The requirements for specific surface functionalization of assay platforms are continuously increasing. The reasons for this increase is the demand for highly sensitive assays, as well as the reduction of non-specific adsorption from complex samples, and with it high signal-to-noise-ratios. To achieve this, different support materials were adapted to the immobilized biomarker/ligand, allowing a high binding capacity and immobilization efficiency. In the case of immunoassays, the immobilized ligands are proteins, antibodies or peptides, which exhibit a diversity of chemical properties (acidic/alkaline; hydrophobic/hydrophilic; secondary or tertiary structure/linear). Consequently it is more challenging to develop immobilization strategies necessary to ensure a homogenous covered surface and reliable assay in comparison to DNA immobilization. New developments concerning material support for each platform are discussed especially with regard to increase the immobilization efficiency and reducing the non-specific adsorption from complex samples like serum and cell lysates.

show abstract

Serodiagnosis of Lyme borreliosis infection using surface plasmon resonance

Cited by 16 publications

References 17 publications

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

4-Aminophenyl boronic acid modified gold platforms for influenza diagnosis

Validation Processes of Protein Biomarkers in Serum—A Cross Platform Comparison

Contact Info

Product

Resources

About