Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Byrne, Barry; Stack, Edwina; Gilmartin, Niamh; O’Kennedy, Richard

doi:10.3390/s90604407

Cited by 330 publications

(207 citation statements)

References 184 publications

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“…Enhancement of the method sensitivity is based, first of all, on the specific interaction of EBV antibodies, circulating in the blood serum, with the antigen (EBV itself) immobilized on the gold film surface. The use of immune molecular reaction of the antigen-antibody interaction as the basis for biosensor design allows to obtain uniquely specific responses and to perform analyses of the complex biological samples such as urine, saliva, blood or serum [13].…”

Section: Resultsmentioning

confidence: 99%

Optimization of surface plasmon resonance based biosensor for clinical diagnosis of the Epstein–Barr herpes virus disease

Khrystosenko¹

2016

Semicond. Phys. Quantum Electron. Optoelectron.

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Abstract. The influence of the sensitive element fabrication technology and noise performance of surface plasmon resonance (SPR) immunosensor on sensitivity and stability of operation inherent to the "Plasmon" series instrument has been investigated to improve reliability of diagnosis and treatment of the Epstein-Barr herpes virus disease. To minimize the effect of the temperature change induced noise, compensation (introduction of the reference channel) and stabilization (active thermal control) methods were applied, allowing to substantially enhance resolution of the used SPR sensor system.

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Section: Resultsmentioning

confidence: 99%

Optimization of surface plasmon resonance based biosensor for clinical diagnosis of the Epstein–Barr herpes virus disease

Khrystosenko¹

2016

Semicond. Phys. Quantum Electron. Optoelectron.

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“…However, the equipment required can be bulky and expensive, but emerging technologies will lead to significant reductions in size. SPR assays have found many applications in different fields such as biomedical diagnostics [88,89], and drug discovery [90,91]. …”

Section: Direct Optical Detectionmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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“…This can be achieved using nanomaterials which, due to their large surface area, allow a greater number of DNA strands to be immobilised (6). Nanomaterials can be incorporated into many types of biosensor configurations to develop magnetic, optical, electrical or electrochemical biodevices for the detection of many biological molecules including nucleic acids, antibodies, proteins, toxins and bacteria (7)(8)(9)(10)(11)(12)(13) The first biosensors were reported in the early 1960's, where a pH response for a 10 mg per cent solution of glucose was reported (14). Since then there have been many advances made in the field, and devices are now more sensitive and more portable.…”

Section: Introductionmentioning

confidence: 99%

The increasing importance of carbon nanotubes and nanostructured conducting polymers in biosensors

et al. 2010

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The growing needs for analytical devices requiring smaller sample volumes, decreased power consumption and improved performance have been driving forces behind the rapid growth in nanomaterials research. Due to their dimensions, nanostructured materials display unique properties not traditionally observed in bulk materials. Characteristics such as increased surface area along with enhanced electrical/optical properties make them suitable for numerous applications such as nanoelectronics, photovoltaics and chemical/biological sensing. In this review we examine the potential that exists to use nanostructured materials for biosensor devices.By incorporating nanomaterials, it is possible to achieve enhanced sensitivity, an improved response time and smaller size. Here we report some of the success that has been achieved in this area. Many nanoparticle and nanofibre geometries are particularly relevant, in this paper however we specifically focus on organic nanostructures, reviewing conducting polymer nanostructures and carbon nanotubes.

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Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Cited by 330 publications

References 184 publications

Optimization of surface plasmon resonance based biosensor for clinical diagnosis of the Epstein–Barr herpes virus disease

Optimization of surface plasmon resonance based biosensor for clinical diagnosis of the Epstein–Barr herpes virus disease

Trends and Perspectives in Immunosensors

The increasing importance of carbon nanotubes and nanostructured conducting polymers in biosensors

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