Serial studies of autologous antibody reactivity to melanoma. Relationship to clinical course and circulating immune complexes.

Vlock, Daniel R.; Kirkwood, John M.

doi:10.1172/jci112042

Cited by 25 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The etiology of the first two drops is not clear but may be the result of treatment with surgery and radiotherapy, respectively. The third drop in antibody titer may be due to treatment with interferon as has been reported previously (22). Of additional note is the preterminal rise in both native (PRE) and AD&U (POST) antibody titers.…”

Section: Isolation Ofmelanoma Antigenssupporting

confidence: 59%

“…We have previously reported that this technique is successful in enhancing autologous antibody reactivity in 9 of 10 cases studied (20,22). Serial studies of autologous antibody reactivity, in native and dissociated sera, have demonstrated correlations between clinical course and disease progression (22). In addition, we have found that the presence host-derived antibody reactivity against an allogeneic melanoma cell line is a predictor of eventual disease recurrence (23).…”

Section: Introductionmentioning

confidence: 94%

“…To pursue this we modified a technique reported by Sjorgen et al (21) to dissociate antibody from antigen in the sera of patients with melanoma. We have previously reported that this technique is successful in enhancing autologous antibody reactivity in 9 of 10 cases studied (20,22). Serial studies of autologous antibody reactivity, in native and dissociated sera, have demonstrated correlations between clinical course and disease progression (22).…”

Section: Introductionmentioning

confidence: 94%

“…Sterile tumor specimens were obtained, established, and maintained in a manner reported previously (20,22 Acid dissociation and ultrafiltration (AD& U)' The method, initially described by Sjogren et al (21), was used to dissociate immune complexes. Briefly, a 60-ml ultrafiltration chamber and Amicon HP-30 membrane (Amicon Corp., Danvers, MA) were used.…”

Section: Cell Linesmentioning

confidence: 99%

“…The ultrafiltrate, obtained by acid dissociation and ultrafiltration, was concentrated by lyophilization. Preliminary work (22) and the absorption analysis illustrated in Fig. 1 gous melanoma cell line Y-Mel 84:420.…”

Section: Isolation Ofmelanoma Antigensmentioning

confidence: 99%

See 4 more Smart Citations

Isolation and partial characterization of melanoma-associated antigens identified by autologous antibody.

Vlock¹,

Scalise²,

Meglin³

et al. 1988

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

The study of the autologous immune response to cancer avoids the difficulties encountered in the use of xenoantisera and may identify antigens of physiological relevance. However, the low titer and incidence of autologous antibody to melanoma have hampered further evaluation. By utilizing acid dissociation and ultrafiltration of serum, we have been able to augment the detectable autologous immune response to melanoma in the majority of patients studied. In autologous system Y-Mel 84:420, serum S150 demonstrated a rise in titer from 1:32 in native sera to 1:262,044 after dissociation. The antigen detected by S150 was found to be broadly represented on melanoma, glioma, renal cell carcinoma, neuroblastoma, and head and neck carcinoma cell lines. It did not react with bladder or colon carcinoma, fetal fibroblasts, pooled platelets, lymphocytes and red blood cells, or autologous cultured lymphocytes. Using polyacrylamide gel electrophoresis, S150 detects a 66,000-mol wt antigen in spent tissue culture media and serum ultrafiltrate. In cell lysate two bands between 20,000 and 30,000 mol wt are detected by S150. The 66,000-mol wt antigen is sensitive to trypsin digestion and but is resistant to pepsin and heat inactivation. Exposure of spent media to trypsin results in the development of a 24,000-mol wt band that appears to correspond to the antigen detected in the cell lysate. The difference between the antigens detected in the cell lysate as compared with spent media and serum ultrafiltrate may be due to degradation during cell lysis. We conclude that melanoma-associated antigens are present in the serum of patients with melanoma and are shed or secreted by their tumor cells.

show abstract

Section: Isolation Ofmelanoma Antigenssupporting

confidence: 59%