Sequential distribution of the mycotoxin deoxynivalenol in wheat spikes after inoculation with Fusarium graminearum

The infection biology of Fusarium graminearum: Defining the pathways of spikelet to spikelet colonisation in wheat ears

“…DON may initiate PCD of the wheat host cells once a certain concentration has been reached. The earlier study by Savard et al (2000) identified a greater accumulation of DON in rachis tissue than the actual spikelets, indicating that within the ear the host cells residing in the different tissue types are experiencing different DON concentrations. In the present study, intracellular hyphae were never seen within host cells that possessed organelles.…”

Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

“…Milled samples from field plots were used as is. DON analysis was performed on F. graminearum-infected tissues using a DON-specific antibody and ELISA analysis [38]. The DON concentration is the mean of two technical replicates.…”

Fusarium Mycotoxins and Their Role in Plant–Pathogen Interactions

“…If UDP-glucosyltransferases with the right specificity are rapidly transcriptionally induced and successfully translated (at lower DON concentrations due to diffusion) the plant can inactivate the toxin, while close to the source of the toxin, translation is most likely blocked to a large extent, so that the pathogen can suppress or delay the defense responses. Based on the in vitro translation data using wheat germ extracts [99,105], 50 % inhibition is reached already at a concentration of about 1.5 µM (400 µg/L), whereas DON concentrations in the inoculated spikelets and spikelets below the inoculation point may reach more than 1000-fold higher levels 4 days after inoculation [106].…”