Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Long, Xiangyu; Balcerzak, Margaret; Gulden, Sigrun; Cao, Wenguang; Fedak, George; Wei, Yuming; Zheng, Youliang; Somers, Daryl J.; Ouellet, Thérèse

doi:10.1016/j.pmpp.2015.02.002

Cited by 16 publications

(34 citation statements)

References 56 publications

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“…Pathological experiments provided further evidence for the function of this QTL. In a recent research by Long et al [ 46 ], visual and microscopic observations were carried out in two pairs of sister lines differing at the 2DLc QTL from Wuhan-1, and the results demonstrated that the QTL suppressed fungal infection in inoculated spikelets and reduced spread from spikelets to rachis. Our previous results indicated the frequent occurrence of this QTL in CIMMYT germplasm [ 22 , 24 ], most likely due to the wide use of Wuhan-1 and Shanghai-3 in the breeding programs.…”

Section: Discussionmentioning

confidence: 99%

QTL Characterization of Fusarium Head Blight Resistance in CIMMYT Bread Wheat Line Soru#1

Lillemo

Shi

et al. 2016

PLoS ONE

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Fusarium head blight (FHB) resistant line Soru#1 was hybridized with the German cultivar Naxos to generate 131 recombinant inbred lines for QTL mapping. The population was phenotyped for FHB and associated traits in spray inoculated experiments in El Batán (Mexico), spawn inoculated experiments in Ås (Norway) and point inoculated experiments in Nanjing (China), with two field trials at each location. Genotyping was performed with the Illumina iSelect 90K SNP wheat chip, along with a few SSR and STS markers. A major QTL for FHB after spray and spawn inoculation was detected on 2DLc, explaining 15–22% of the phenotypic variation in different experiments. This QTL remained significant after correction for days to heading (DH) and plant height (PH), while another QTL for FHB detected at the Vrn-A1 locus on 5AL almost disappeared after correction for DH and PH. Minor QTL were detected on chromosomes 2AS, 2DL, 4AL, 4DS and 5DL. In point inoculated experiments, QTL on 2DS, 3AS, 4AL and 5AL were identified in single environments. The mechanism of resistance of Soru#1 to FHB was mainly of Type I for resistance to initial infection, conditioned by the major QTL on 2DLc and minor ones that often coincided with QTL for DH, PH and anther extrusion (AE). This indicates that phenological and morphological traits and flowering biology play important roles in resistance/escape of FHB. SNPs tightly linked to resistance QTL, particularly 2DLc, could be utilized in breeding programs to facilitate the transfer and selection of those QTL.

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Section: Discussionmentioning

confidence: 99%

QTL Characterization of Fusarium Head Blight Resistance in CIMMYT Bread Wheat Line Soru#1

Lillemo

Shi

et al. 2016

PLoS ONE

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“…Therefore, the 2DL-mediated resistance appears to act by antagonizing the interference of phytohormone pathways by the pathogen. Interestingly, significant differences observed between resistant and susceptible NILs in basal expression levels of a subset of defence genes were not affected by F. graminearum, suggesting that the 2DL-mediated resistance may also be mediated by increased levels of basal defences (Long et al, 2015). Indeed, metabolomic analyses have shown that basal levels of various resistance-related metabolites are higher in resistant than susceptible NILs (Hamzehzarghani et al, 2008).…”

Section: DLmentioning

confidence: 99%

“…The 2DL QTL, derived from the wheat cultivar Wuhan-1, provides FHB resistance by reducing the spread of the pathogen within the head, which is termed type 2 resistance (Mesterhazy, 1995). The mode of action of 2DL has been investigated using NILs that differ by this locus (Long et al, 2015). Strong down-regulation of phytohormone-regulated genes, including those involved in auxin signalling, was observed in the susceptible NIL (Long et al, 2015).…”

Section: DLmentioning

confidence: 99%

“…The mode of action of 2DL has been investigated using NILs that differ by this locus (Long et al, 2015). Strong down-regulation of phytohormone-regulated genes, including those involved in auxin signalling, was observed in the susceptible NIL (Long et al, 2015). Therefore, the 2DL-mediated resistance appears to act by antagonizing the interference of phytohormone pathways by the pathogen.…”

Section: DLmentioning

confidence: 99%

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Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Kazan

Gardiner

2017

Molecular Plant Pathology

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The ascomycete fungal pathogen Fusarium graminearum causes the globally important Fusarium head blight (FHB) disease on cereal hosts, such as wheat and barley. In addition to reducing grain yield, infection by this pathogen causes major quality losses. In particular, the contamination of food and feed with the F. graminearum trichothecene toxin deoxynivalenol (DON) can have many adverse short- and long-term effects on human and animal health. During the last decade, the interaction between F. graminearum and both cereal and model hosts has been extensively studied through transcriptomic analyses. In this review, we present an overview of how such analyses have advanced our understanding of this economically important plant-microbe interaction. From a host point of view, the transcriptomes of FHB-resistant and FHB-susceptible cereal genotypes, including near-isogenic lines (NILs) that differ by the presence or absence of quantitative trait loci (QTLs), have been studied to understand the mechanisms of disease resistance afforded by such QTLs. Transcriptomic analyses employed to dissect host responses to DON have facilitated the identification of the genes involved in toxin detoxification and disease resistance. From the pathogen point of view, the transcriptome of F. graminearum during pathogenic vs. saprophytic growth, or when infecting different cereal hosts or different tissues of the same host, have been studied. In addition, comparative transcriptomic analyses of F. graminearum knock-out mutants with altered virulence have provided new insights into pathogenicity-related processes. The F. graminearum transcriptomic data generated over the years are now being exploited to build a systems level understanding of the biology of this pathogen, with an ultimate aim of developing effective and sustainable disease prevention strategies.

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“…The RNA-Seq analysis of Wangshuibai and its FHB susceptible mutant with deletion of the QTL-Fhb1 region identified several differentially expressed genes but none were selected to have rachis resistance (Xiao et al, 2013). Recently, gene expression profiling of NILs containing QTL-2DL revealed eight candidate genes but only one gene was localized on the 2DL chromosome (Long et al, 2015). Although several candidate genes were identified in most of the studies, none could pinpoint and explain the resistance mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a fusarium head blight resistance gene TaACT in wheat QTL‐2DL

Kage

Karre

Kushalappa

et al. 2016

Plant Biotechnology Journal

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SummaryFusarium head blight (FHB) resistance in wheat is considered to be polygenic in nature. Cell wall fortification is one of the best resistance mechanisms in wheat against Fusarium graminearum which causes FHB. Metabolomics approach in our study led to the identification of a wide array of resistance‐related (RR) metabolites, among which hydroxycinnamic acid amides (HCAAs), such as coumaroylagmatine and coumaroylputrescine, were the highest fold change RR metabolites in the rachis of a resistant near‐isogenic line (NIL‐R) upon F. graminearum infection. Placement of these metabolites in the secondary metabolic pathway led to the identification of a gene encoding agmatine coumaroyl transferase, herein referred to as TaACT, as a candidate gene. Based on wheat survey sequence, TaACT was located within a FHB quantitative trait loci on chromosome 2DL (FHB QTL‐2DL) between the flanking markers WMC245 and GWM608. Phylogenetic analysis suggested that TaACT shared closest phylogenetic relationship with an ACT ortholog in barley. Sequence analysis of TaACT in resistant and susceptible NILs, with contrasting levels of resistance to FHB, led to the identification of several single nucleotide polymorphisms (SNPs) and two inversions that may be important for gene function. Further, a role for TaACT in FHB resistance was functionally validated by virus‐induced gene silencing (VIGS) in wheat NIL‐R and based on complementation studies in Arabidopsis with act mutant background. The disease severity, fungal biomass and RR metabolite analysis confirmed TaACT as an important gene in wheat FHB QTL‐2DL, conferring resistance to F. graminearum.

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Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Cited by 16 publications

References 56 publications

QTL Characterization of Fusarium Head Blight Resistance in CIMMYT Bread Wheat Line Soru#1

QTL Characterization of Fusarium Head Blight Resistance in CIMMYT Bread Wheat Line Soru#1

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Identification and characterization of a fusarium head blight resistance gene TaACT in wheat QTL‐2DL

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