Sequences of Tobacco Rattle Viruses from Potato

Abstract

The RNA2 of the tobacco rattle virus (TRV) isolate ‘Rostock’ (TRV‐R), and the coat protein gene (CP) of TRV isolate ‘Mirow’ (TRV‐M) were sequenced. Both were isolated from potato. Sequence analysis revealed a 5′ noncoding‐region (NCR) and a CP gene, which are among the shortest of any tobravirus RNA2 sequenced so far. Downstream of the CP open reading frame (ORF) there was an ORF for a 8 k protein, that is probably a truncated 9 k protein, followed by an ORF for a 16 k protein and the 3′ NCR. The 16 k protein and the 3′ NCR showed a considerable sequence identity with the 3′ end of TRV RNA1 and RNA2 from other TRV isolates. The high identity in the amino acid sequences of the CPs from TRV‐R and TRV‐M as well as other TRV isolates suggested that they are related to the TCM group of the genus Tobravirus and belong serologically to the RQ‐serotype. This could be confirmed using a rabbit antiserum that was prepared against recombinant TRV‐R CP expressed in bacteria. This serum was found to be suitable for differentiation of TRV‐isolates.

Advancing Virus-Induced Gene Silencing in Sunflower: key factors of VIGS spreading and a novel simple protocol

“…The final aspect related to VIGS that we investigated in our study was the mobility of TRV in various regions and tissues of infected plants. TRV belongs to the Tobravirus genus [ 52 ]. Viruses in this classification have two genomes, each consisting of a positivesense single-stranded RNA (+ssRNA) [ 53 , 54 ].…”

A Subgroup 1 Tobamovirus Isolated from Brugmansia sp. and its Detection by RT‐PCR

“…Coat protein from purified particles was separated on a SDS‐PAGE according to Laemmli (1970) and stained with Coomassie Brilliant Blue. Western‐blotting was carried out as described in Heinze et al. (1998).…”

Detection, distribution and control of Potato mop‐top virus, a soil‐borne virus, in northern Europe

“…To test whether the spraing symptoms observed in Poland were caused by TRV, tubers were tested by RT‐PCR using TRV‐specific primers targeting RNA1 (the 16K gene) of the TRV genome (Heinze et al , 2000), because the viral RNA2, which contains CP , may be missing from TRV isolates that infect potatoes (Weidemann, 1995). TRV was detected in the spraing‐expressing tubers of the cultivars tested, including Dali, Ditta, Fontana, Folva, Innovator, Rudawa and Tajfun, but not in leaves of the plants grown from infected tubers, e.g.…”