Sequence of the cob A gene encoding : uroporhyrinogen III methyltransferase of Pseudomonas fluorescens

Mot, René De; Schoofs, Geert; Nagy, István; Vanderleyden, Jos

doi:10.1016/0378-1119(94)90886-9

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Interestingly, in R. meliloti, the structural carrier gene dctA, and the regulatory genes dctB and dctD, are organized in a single operon (Watson, 1990), but in both P. fluorescens SBW25 and P. fluorescens 2-79, dctA is not contiguous with dctBD (Challis, 1994), and in SBW25, dctA is located approximately 3 Mbp away from dctBD. In P. fluorescens OE 28.3, oprF and cobA are tightly linked (De Mot et al, 1994), and the results of separate Southern hybridization analysis with both genes indicate that a similar arrangement exists in SBW25. The genes involved in pilus biosynthesis and twitching motility (pilGHIJ ) are arranged in a single operon in P. aeruginosa, and, while separate probes were not used for each gene, the hybridization of only a single SpeI fragment (SpB) by pADD698 suggests that at least one of these genes (and possibly all four) are clustered in SBW25.…”

Section: Discussionmentioning

confidence: 92%

Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome

Rainey

Bailey²

1996

Molecular Microbiology

197

162

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Pseudomonas fluorescens is a saprophytic bacterium commonly isolated from soil, water, and the surfaces and tissues of plants and animals. The species has important applications in biotechnology because it can enhance plant growth and protect crops against disease. A complete physical map of the 6.63 Mbp P. fluorescens SBW25 chromosome was constructed using data obtained from combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA with end labelling. In total, 139 restriction sites (15 PacI, 53 SpeI, 71 XbaI) were placed on the physical map and complete maps of the circular chromosome were obtained for both PacI and SpeI; only XbaI fragments linking SpeI fragments were positioned. The average resolution of restriction sites was 48 kbp. A genetic map was derived from the physical map by southern hybridization and 31 genes were positioned including oriC, rDNA operons (rnnA-E), recA, gacA, and pyvD.

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Section: Discussionmentioning

confidence: 92%

Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome

Rainey

Bailey²

1996

Molecular Microbiology

197

162

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“…The nirE gene product showed a high level of identity with the Uro'genIII methylase identified in some species, including CysG from E. coli (32) and CobA from Pseudomonas denitrificans (5,11). Uroporphyrinogen III is a precursor of tetrapyrrole biosynthetic reactions (7).…”

Section: Discussionmentioning

confidence: 99%

Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis

et al. 1997

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“…[13][14][15] For the other ORFs, the deduced amino acid sequence of the incomplete ORF1 showed similarity to the C-terminal part of bacterial nitrate reductases, e.g., Sphingomonas elodea NasA (64%, AY220099) and Rhodobacter capsulatus NasA (46%, AY273169), whereas the sequence of the ORF3 exhibited similarity to those of the N-terminal part of molybdopteringuanine dinucleotide biosynthesis synthases found in Erythrobacter litoralis (60%, CP000157) and Novosphingobium aromaticivorans (56%, CP000248). UPMTase is a key enzyme in the synthesis of siroheme, a prosthetic group found in nitrate reductases.…”

Section: Gene Analysismentioning

confidence: 99%