“…To access the uncultured majority of the microbial world (43), functional metagenomic approaches that allow for the expression of environmental DNA from uncultured organisms in surrogate hosts have been developed (6,22,32,35,36,46). Functional screening of metagenomic libraries has led to the discovery of several novel bioactives and metabolic pathways (5,36,52), but the search for new antibiotics has focused mainly on soil-derived samples.…”