Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins

Thiamine and the Cellular Energy Cycles — A Novel Perspective on Type 2 Diabetes Treatment

“…assessment of mononuclear TK activity and plasma thiamine concentration determination using HPLC flourimetric determination with respect to normal healthy controls gives greater insight into thiamine status [185][186][187]. More recently in capillary enzyme reaction and capillary electrophoresis methods are emerging as potential alternative monitoring and determining techniques for thiamine in samples [188].…”

Multicomponent Analysis of Fat- and Water-Soluble Vitamins and Auxiliary Substances in Multivitamin Preparations by qNMR

“…Several HPLC methods have been proposed to determine water-soluble vitamins using corona-charged aerosol and diode array detection. In these methods, the mobile phase usually consists of water, buffer solutions (ammonium acetate or dihydrogen phosphate), and a proportion of organic solvents (e.g., methanol or acetonitrile). , Other successful methods included tandem mass spectrometry, electrokinetic chromatography, and capillary electrophoresis. − On the contrary, fat-soluble vitamins (i.e., A, D, E, and K) can be analyzed by means of a reversed-phase HPLC procedure with the mobile phase consisted of a mixture of methanol with acetonitrile, supercritical fluid chromatography (SFC), or by microemulsion electrokinetic chromatography (MEEKC). − …”

Determination of Water-Soluble Vitamins B1, B2, B3, B6, B9, B12 and C on ODS Column 5 μm by High Performance Liquid Chromatography with UV-DAD Detection

“…The simultaneous determination of several watersoluble vitamins is difficult and often many different analyses have been performed. Water-soluble vitamins have been determined by various instrumental methods such as fluorescence 2 which can determine a small concentration of vitamins but it concerns only fluorescent vitamins and it coast lot of time and power, microbiological methods 3 which are typically designed for single vitamin analysis and time consuming, electrochemical assays 4 , immunoassays 5,6 and chromatographic methods, including gas chromatography 7 , capillary electrophoresis [8][9][10] , thin-layer chromatography 11 and liquid chromatography (LC) with various method for the simultaneous determination of multiple vitamins in food or multivitamin tablets because of its high sensitivity available detection [12][13][14] . Among these methods, HPLC is a promising and specificity for separation and determination mixture of vitamins with several detections such as electrochemical detection 15 , mass spectroscopy 16 , fluorescence 17,18 and UV, visible detection [19][20][21][22] .…”