Determination of Water-Soluble Vitamins B1, B2, B3, B6, B9, B12 and C on ODS Column 5 μm by High Performance Liquid Chromatography with UV-DAD Detection

Antakli, Saad; Sarkees, Nazeera; Sarraf, Tereza

doi:10.14233/ajchem.2014.17814

Cited by 6 publications

(4 citation statements)

References 20 publications

(20 reference statements)

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“…Vitamin C (Ascorbic acid), a water-soluble vitamin, was measured according to Antakli et al . 31 , by reversed phase-liquid chromatography with UV detection (λ em 246 nm) using BDS-HYPERSIL-C18 column (100 × 4.6 mm, 3 µm, Thermo Fisher Scientific Inc., USA) with mobile phase of (A): 5.84 mM of hexane-1-sulfonic acid sodium: acetonitrile (95:5, v/v) with 0.1% triethylamine, (B): 5.84 mM of hexane-1-sulfonic acid sodium: acetonitrile (50:50) with 0.1% triethylamine, both 2.5 pH adjusted using 1 M orthophosphoric acid. On the other hand, Vitamin E (α-tocopherol), fat-soluble vitamin, was measured according to Barba et al .…”

Section: Methodsmentioning

confidence: 99%

Impact of cow, buffalo, goat or camel milk consumption on oxidative stress, inflammation and immune response post weaning time

Amr,

Farid

2024

Sci Rep

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Milk is a whitish liquid that is secreted from mammary glands; and considered as the primary source of nutrition for newborns since they are not able to digest solid food. However, it contains primary nutrients, as well as growth and immune factors. Early weaning is a critical issue that face women and their babies in developing countries. To avoid infant malnutrition, they tend to use other milk types instead of baby formula. Therefore, the present study aimed to evaluate the impact of cow, buffalo, goat or camel milk consumption on oxidative stress, inflammation and immune response in male and female Sprague Dawley rats post weaning time. The amino acids, fatty acids, minerals and vitamins in the tested milk types were evaluated. Animals were divided into 5 groups (control, cow, buffalo, goat and camel milk administrated groups) (10 rats/group); each animal was administrated by 3.4 ml/day. Rats were administered with milk for 6 weeks; at the end of the 5th week, five animals of each group were isolated and the remaining five animals were immunized with sheep red blood cells (SRBCs) and kept for another week to mount immune response. The effect of different milk types on rats’ immune response towards SRBCs was evaluated through pro-inflammatory cytokines, antioxidants, ESR and CRP measurement; together, with the histopathological examination of spleen samples and hemagglutination assay. Camel milk consumption reduced oxidative stress and inflammation in spleen that resulted from SRBCs immunization; in addition to, B cell stimulation that was apparent from the high level of anti-SRBCs antibodies. Camel milk is recommended for newborn consumption, due to its high-water content, unsaturated fatty acids, and vitamin C, as well as low lactose and fat content.

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Section: Methodsmentioning

confidence: 99%

Impact of cow, buffalo, goat or camel milk consumption on oxidative stress, inflammation and immune response post weaning time

Amr,

Farid

2024

Sci Rep

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“…Vitamin B complexes were fractionated according to the method defined by Antakli et al [ 65 ], with a slight modification using a variable wavelength detector (VWD) instead of a fluorescence detector with the VWD set at 280 nm. This method was validated by following some details, including LOD, LOQ and recovery range (97–100%) [ 65 ].…”

Section: Methodsmentioning

confidence: 99%

Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

Ali,

Ibrahim,

Salah-Eldin

2024

Molecules

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Phytochemicals from waste materials generated by agricultural and industrial processes have become globally significant due to their accessibility and potential effectiveness with few side effects. These compounds have essential implications in both medicine and the economy. Therefore, a quantitative analysis of the phytochemical profile, sugar types, and water-soluble vitamins of dried Corchorus olitorius L.“DJMS” extract (dried Jew’s mallow stem) was carried out with HPLC. In addition, the chemical composition, TPC, chlorophyll a and b, beta-carotene, and antioxidant effect using DPPH were investigated. Furthermore, the anticancer activity of the DJMS was evaluated by SRB assay using Huh-7 and MDA-MB-231 cell lines. In the quantitative study, DJMS extract showed a high antioxidant potential (67%) due to its content of bioactive compounds such as TPC (276.37 mg 100 g−1) and chlorophyll a and b (20.31, 12.02 mg 100 g−1, respectively), as well as some vitamins and minerals such as B-complex (B12; 146.8 mg 100 g−1 and vitamin C 6.49 mg 100 g−1) and selenium (<0.2 μg kg−1). Moreover, the main sugar types found were sucrose and stachyose, which recorded 9.23 and 6.25 mg 100 g−1, respectively. Identifying phenolic and flavonoids showed that the major components were ellagic acid (4905.26 μg kg−1), ferulic acid (3628.29 μg kg−1), chlorogenic acid (3757.08 μg kg−1), luteolin—7-O-glucoside (4314.48 μg kg−1), naringin (4296.94 μg kg−1) and apigenin—6—rhamnose—8 glucoside (3078.87 μg kg−1). The dried stem extract showed significant MDA-MB-231 inhibition activity and reached 80% at a concentration of 1000 µg/mL of DJMS extract, related to the content of phytochemical components such as isoflavones like genistein (34.96 μg kg−1), which had a tremendous anticancer effect. Hence, the stem of Jew’s mallow (which is edible and characterized by its viability and low production cost) possesses the capacity to serve as a pharmaceutical agent for combating cancer owing to its abundance of bioactive components.

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“…A gradient flow was started with 100% of solvent A until the mobile composition, then changed to 50% of A and 50% of B for 5 min. The UV-PDA (photodiode array) wavelength absorbance detector was set at 455 nm (vitamin A), 246 nm (vitamins C and B1), 267 nm (vitamin B2), 260 nm (vitamin B3), 204 nm (vitamin B5), 290 nm (vitamin B6), 282 nm (vitamin B9), 280 nm (vitamin D), and 290 nm (vitamin E) [20,21]. System suitability was considered with respect to instrument precision, resolution, tailing factor, and retention time.…”

Section: Vitamin Analysismentioning

confidence: 99%

Bioactive and Physicochemical Characteristics of Natural Food: Palmyra Palm (Borassus flabellifer Linn.) Syrup

Chiu

Chan

et al. 2021

Biology

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Palmyra palm syrup, produced from Borassus flabellifer flowers’ sap, is rich in nutrients and minerals and has unique flavors. This study evaluated the in vitro antioxidant activity, physicochemical characteristics, and Maillard reaction products of palmyra palm syrup prepared by thermal and ultrafiltration processes. Palmyra palm syrup prepared by a thermal process had smaller L*, b* values, and larger a* values than that prepared by an ultrafiltration process. Palmyra palm syrup contained 10 vitamins, the most abundant being vitamin E. Overall, 38 volatile compounds were found and classified into six groups in the order of alcohols > acids > ketones > sulfurs > pyrazines > phenols and aldehyde. Volatile compounds depended on concentration, temperature, and ultrafiltration process. Protein content decreased because of participation in the Maillard reaction and increased 5-hydroxymethylfurfural (HMF) and total phenolic content. The HMF content was very low (0.02–14.95 mg/100 g). The radical scavenging activity of 2,2-diphenyl-1-1 picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) in palmyra palm syrup with thermal process was higher than with ultrafiltration. This study established that ultrafiltration pretreatment of palmyra palm syrup generated a good appearance and reduced the HMF content, however, it negatively affected the volatile compounds and physicochemical characteristics.

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Determination of Water-Soluble Vitamins B1, B2, B3, B6, B9, B12 and C on ODS Column 5 μm by High Performance Liquid Chromatography with UV-DAD Detection

Cited by 6 publications

References 20 publications

Impact of cow, buffalo, goat or camel milk consumption on oxidative stress, inflammation and immune response post weaning time

Impact of cow, buffalo, goat or camel milk consumption on oxidative stress, inflammation and immune response post weaning time

Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

Bioactive and Physicochemical Characteristics of Natural Food: Palmyra Palm (Borassus flabellifer Linn.) Syrup

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