Separation of Ternary Mixtures by Pseudo‐Simulated Moving‐Bed Chromatography: Separation Region Analysis

Graça, Nuno S.; Pais, L.S.; Rodrigues, Alı́rio E.

doi:10.1002/ceat.201500157

Cited by 8 publications

(8 citation statements)

References 34 publications

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“…It may also be possible to adapt

Ψ

and

Ω_{i}

to aid design of non‐conventional SE‐SMB operating schemes (e.g., in Refs. ). However, MP phenomena do not occur in most multi‐column batch processes (e.g., in Ref.…”

Section: Discussionmentioning

confidence: 97%

Modulator Dynamics Shape the Design Space for Stepwise‐Elution Simulated Moving Bed Chromatographic Separations

Wayne

Velayudhan

2018

Biotechnology Journal

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For proteins and other biological macromolecules, SMB chromatography is best operated non-isocratically. However, traditional modes of non-isocratic SMB operation generate significant mobile-phase modulator dynamics. The mechanisms by which these modulator dynamics affect a separation's success, and thus frame the design space, have yet to be explained quantitatively. Here, the dynamics of the modulator (e.g., salts in ion exchange and hydrophobic interaction chromatography) are explicitly accounted for. This leads to the elucidation of two new design constraints, presented as dimensionless numbers, which quantify the effects of the modulator phenomena and thus predict the success of a non-isocratic SMB separation. Consequently, these two new design constraints re-define the SMB design space. Computational and experimental studies at the boundaries of this design space corroborate the theoretical predictions. The design of efficient and robust operating conditions through use of the new design space is also demonstrated.

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“…It may also be possible to adapt

Ψ

and

Ω_{i}

to aid design of non‐conventional SE‐SMB operating schemes (e.g., in Refs. ). However, MP phenomena do not occur in most multi‐column batch processes (e.g., in Ref.…”

Section: Discussionmentioning

confidence: 97%

Modulator Dynamics Shape the Design Space for Stepwise‐Elution Simulated Moving Bed Chromatographic Separations

Wayne

Velayudhan

2018

Biotechnology Journal

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“…It determines the parameters that should be kept fixed in each series of experiments and is carried out until the highest magnitude reaches a predefined Cutoff Value (CV). The principle ranks the sensitivity of parameters based on their magnitude, M (p i ), given by 49 (8) where S pi is the p-th column of matrix S.…”

Section: M2: Estimability Analysismentioning

confidence: 99%

Strategies for Simulated Moving Bed Model Parameter Estimation Based on Minimal System Minimal Knowledge: Adsorption Isotherm Equation Screening and Estimability Analysis

Santos,

Rebello,

Prudente

et al. 2023

Ind. Eng. Chem. Res.

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The simulated moving bed (SMB) is a widely recognized technique for the resolution of several high-value-added compounds, and its mathematical modeling plays a crucial role in predicting its behavior. However, modeling can be challenging as it needs parameter estimation and topology definition, especially for such complex systems. SMB modeling might incorporate different adsorption isotherm equations (AIEs), which might better represent the behavior of a certain mixture to be separated. This work proposes two methodologies for SMB modeling and AIE selection relying on minimal system knowledge that can provide a representative model by a global parameter estimation instead of the traditional "Step by Step" approach. The first methodology proposes an architecture for global parameter estimation that encompasses the screening of the most suitable AIE in the lower level and the definition of the minimum number of experiments necessary for model identification in the upper level. In the second methodology, an extended generic isotherm, a combination of AIE, is formulated; and an estimability analysis is carried out to determine the estimable parameters. Both methodologies apply Latin Hypercube Sampling (LHS) for the design of experiments and emulation of a real unit by incorporating noise in the experimental data under a Gaussian error distribution. Moreover, uncertainty evaluation and data reconciliation are performed to enhance the robustness, reliability, and precision of the proposed approaches. The methodologies are validated by considering experimental data from the literature, apart from the LHS-generated samples, and are subject to cross-validations. Finally, the two methodologies are compared, with the first methodology demonstrating a superior performance for the case study. This work contributes to the advancement of SMB modeling, providing valuable tools for parameter estimation and AIE selection.

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“…However, the above processes were mainly applied to the three-component systems and rarely involved in the four-component separation systems. [27][28][29] Kim et al 30 proposed the different multizone SMBs combining the characteristics of cascade theory and four-component separation. They studied those continuous processes theoretically, and developed relevant mathematical models.…”

Section: Simulated Moving Bed (Smb) Was Developed Originally By Unive...mentioning

confidence: 99%

“…Furthermore, some other strategies including three‐zone SMB, 23 five‐zone SMB, 24 gradient elution, 25 and partial injection 26 have been proposed for the separation of multiple components. However, the above processes were mainly applied to the three‐component systems and rarely involved in the four‐component separation systems 27–29 . Kim et al 30 proposed the different multizone SMBs combining the characteristics of cascade theory and four‐component separation.…”

Section: Introductionmentioning

confidence: 99%

Design and optimization of JO‐IEX process for highly efficient quaternary separation of 5′‐ribonucleotides

et al. 2022

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The developed JO‐IEX technology was applied to continuously separate four 5′‐ribonucleotides here. The unit contained seven columns with three separation steps, among which three columns were for the quaternary separation and the others for regeneration of resins. The operating conditions, that is, the critical flow rates in three separation steps were preliminarily designed according to the equilibrium theory and optimized by a three‐dimensional theoretical separation region approach. The feasibility of the JO‐IEX process and the separation performances were validated by four runs using the actual 5′‐ribonucleotides' enzymatic hydrolysate as feed solutions. The purities and yields of 5′‐ribonucleotides could reach above 97%. The productivity was enhanced to be 0.91 g/(kg h), and the desorbent consumption was reduced to be 1.01 L/g, compared to those of 0.70 g/(kg h) and 1.75 L/g in the fixed‐bed process. The technology proposed could be a potential technique in the continuous multicomponent ion‐exchange separation process.

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Separation of Ternary Mixtures by Pseudo‐Simulated Moving‐Bed Chromatography: Separation Region Analysis

Cited by 8 publications

References 34 publications

Modulator Dynamics Shape the Design Space for Stepwise‐Elution Simulated Moving Bed Chromatographic Separations

Modulator Dynamics Shape the Design Space for Stepwise‐Elution Simulated Moving Bed Chromatographic Separations

Strategies for Simulated Moving Bed Model Parameter Estimation Based on Minimal System Minimal Knowledge: Adsorption Isotherm Equation Screening and Estimability Analysis

Design and optimization of JO‐IEX process for highly efficient quaternary separation of 5′‐ribonucleotides

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