Design and optimization of JO‐IEX process for highly efficient quaternary separation of 5′‐ribonucleotides

Abstract

The developed JO‐IEX technology was applied to continuously separate four 5′‐ribonucleotides here. The unit contained seven columns with three separation steps, among which three columns were for the quaternary separation and the others for regeneration of resins. The operating conditions, that is, the critical flow rates in three separation steps were preliminarily designed according to the equilibrium theory and optimized by a three‐dimensional theoretical separation region approach. The feasibility of the JO‐IEX process and the separation performances were validated by four runs using the actual 5′‐ribonucleotides' enzymatic hydrolysate as feed solutions. The purities and yields of 5′‐ribonucleotides could reach above 97%. The productivity was enhanced to be 0.91 g/(kg h), and the desorbent consumption was reduced to be 1.01 L/g, compared to those of 0.70 g/(kg h) and 1.75 L/g in the fixed‐bed process. The technology proposed could be a potential technique in the continuous multicomponent ion‐exchange separation process.

Model‐based design of stratified packings for enhanced mass transfer using optimal control theory

“…Diffusive mass transfer between a stationary phase and a liquid phase, as well as various adsorption processes, exist in many different industries, including the pharmaceutical, chemical, and food industries. Chromatographic separation processes, 1,2 adsorption columns, 3–5 coffee extraction, 6,7 and porous media washing 8 are exemplary applications. These processes typically involve homogeneous packings with a constant mean particle diameter along the axial direction.…”