Separation of precolumn ortho-phthalaldehyde-derivatized amino acids by capillary zone electrophoresis with normal and micellar solutions in the presence of organic modifiers

Liu, Jinping; Cobb, Kelly A.; Novotný, Miloš V.

doi:10.1016/s0021-9673(00)96305-2

Cited by 84 publications

(14 citation statements)

References 18 publications

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“…Few native peptides contain a fluorophore, which can be excited by lasers, therefore for a CE-LIF detection system the analyte should be usually derivatized with a fluorescent probe. A series of derivatizing agents, such as fluorescamine [2,3], o-phthaldialdehyde (OPA) [4][5][6], naphthalene-2,3-dicarboxyaldehyde (NDA) [7][8][9], fluorescein isothiocyanate (FITC) [10,11], 3-(4-carboxybenzoyl)-2-quinoline-carboxaldehyde (CBQCA) [9,12,13], and tetramethylrhodamine (TRITC) [14] have been used as fluorescent probes for amino acids and peptides. Some reports [11,13,15,16] have focused on derivatization, separation, and detection of amino acids by CE-LIF.…”

Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic chromatography of enkephalin‐related peptides with laser‐induced fluorescence detection

2004

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Eight enkephalin-related peptides were derivatized using fluorescein isothiocyanate (FITC), and the derivatization products were separated and detected by capillary electrophoresis with laser-induced fluorescence detection. The optimum molar ratio of FITC and peptide for derivatization was found to be 40:1, and 5 mmol/L sodium borate buffer (pH 9.2) was selected as derivatization media in order to get the high efficiency. Enkephalin-related peptides were completely separated in the pH range of 10.51-10.60 in a running buffer consisting of 100 mmol/L sodium borate and 60 mmol/L sodium dodecyl sulfate (SDS). The detection limit for these eight enkephalins ranged from 0.18 to 2.25 nmol/L, and the linear response range was 1.0 x 10(-6) to 1.0 x 10(-9) mol/L with correlation coefficients between 0.9947-0.9988. A separation efficiency as high as 380000 theoretical plates could be obtained for these analytes.

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Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic chromatography of enkephalin‐related peptides with laser‐induced fluorescence detection

2004

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“…Derivatisation with OPA offers many advantages [31] and the reaction is well known [32]. O P A shows no fluorescence by itself so a large excess can be used without disturbing system peaks appearing in the electropherogram.…”

Section: Figurementioning

confidence: 99%

“…To enhance resolution of the fluorescent isoindole-compounds, an M E K C separation similar to that described by Novotny et al [31] …”

Section: Figurementioning

confidence: 99%

Determination of inorganic anions, carboxylic acids and amino acids in plant matrices by capillary zone electrophoresis

et al. 1997

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SummaryPhysiological investigations of solute transport in plants affords knowledge of solute distribution between different tissues. Capillary electrophoresis using indirect UV and laser induced fluorescence (LIF) detection is demonstrated as a useful technique for the simultaneous determination of inorganic anions, amino acids and carboxylic acids in plant samples. Cell sap obtained from plant tissues as well as simple ethanolic or aqueous plant extracts can be analysed directly without any pretreatment. This matrix stability and the very small volumes required allow fast determinations of various compounds in small plant tissue sections. In the case of carboxylic acids, resolution can be optimized using calcium for selective complexation of some of the compounds. Selective and sensitive determination of amino acids is possible using precolumn derivatisation with orthophthaldialdehyde (OPA) and laser induced fluorescence detection.

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“…More recently, the approach was extended to include fluorimetric detection in capillary zone electrophoresis (61)(62)(63). In addition, use of OPA and a primary amino compound allows for the fluorimetric determination of different thiol-containing compounds and sulfite ion (49)(50)(51)64).…”

Section: Figmentioning

confidence: 99%

Update and Evaluation of the Effectiveness of Different Thiols and Micellar Media in Roth's Fluorimetric Method for the Determination of Primary Amino Compounds

Dai

Burkert

Singh

et al. 1997

Microchemical Journal

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Separation of precolumn ortho-phthalaldehyde-derivatized amino acids by capillary zone electrophoresis with normal and micellar solutions in the presence of organic modifiers

Cited by 84 publications

References 18 publications

Micellar electrokinetic chromatography of enkephalin‐related peptides with laser‐induced fluorescence detection

Micellar electrokinetic chromatography of enkephalin‐related peptides with laser‐induced fluorescence detection

Determination of inorganic anions, carboxylic acids and amino acids in plant matrices by capillary zone electrophoresis

Update and Evaluation of the Effectiveness of Different Thiols and Micellar Media in Roth's Fluorimetric Method for the Determination of Primary Amino Compounds

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