Update and Evaluation of the Effectiveness of Different Thiols and Micellar Media in Roth's Fluorimetric Method for the Determination of Primary Amino Compounds

Dai, Fang; Burkert, Vera Prelevic; Singh, H.N.; Hinze, Willie L.

doi:10.1006/mchj.1997.1529

Cited by 20 publications

(7 citation statements)

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“…The methods for OPA on surfaces by fluorescent analysis employs Roth's fluorimetric method for determining analytes bearing a primary amino group. 25,26 Under basic conditions, the fluorigenic reagents, OPA, a thiol, and a species containing a primary amino group, undergo reaction to form a thio-N-alkylsubstituted isoindole. In this work, Roth's fluorimetric method for determining primary amino compounds has been turned around for the determination of OPA in lieu of compounds bearing primary amino groups.…”

Section: Methods For Opa In Airmentioning

confidence: 99%

“…of mixing. 25 While the primary fluorescent method employs a portable fluorometer for quantitation, the procedure for the secondary fluorescent method is the same as the primary one except that (1) a UV lamp emitting at 366 nm replaces the fluorometer and (2) the secondary method is employed mainly as a qualitative method for detection (not for measurement). The LOD of OPA is estimated at 0.01 mg mL À1 or 0.12 mg per 12 mL sample.…”

Section: Methods For Opa In Airmentioning

confidence: 99%

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Determination of ortho-phthalaldehyde in air and on surfaces

Tucker

2008

J. Environ. Monit.

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Three sampling and analytical methods have been developed and evaluated for ortho-phthalaldehyde (OPA): (1) an HPLC-UV method for OPA in air, (2) a fluorimetric method for OPA on surfaces, and (3) a colorimetric method for OPA on surfaces. (1) The air sampler contains 350 mg of silica gel coated with 1 mg of acidified 2,4-dinitrophenylhydrazine (DNPH). Air sampling may be conducted at 0.03 to 1.0 L min(-1) for periods up to 8 h. Samples were eluted with ethyl acetate, and the eluents were allowed to stand for 72 h. Analysis was by high performance liquid chromatography (HPLC) with a UV detector set at 369 nm. An unusual phenomenon was the observation that the stability of the sample on a sampler at 3 degrees C tends to decrease as the total quantity of OPA collected on the sampler decreases. Elution of the samples within 24 h of air sampling is required. The detection limit (LOD) is approximately 0.02 microg of OPA per sample. OPA on surfaces may be collected with strips cut from a sheet of polyvinyl alcohol (PVA wipe). (2) In the surface wipe method with analysis by fluorescence measurement, the strips of PVA wipe were placed into dimethyl sulfoxide. An aliquot was treated with aqueous N-acetyl-l-cysteine and ethylenediamine. Analysis was performed with a portable fluorometer (excitation and emission wavelengths = 365 nm and 438 nm, respectively). The LOD is 0.2 microg per sample. (3) In the surface wipe method with visual colorimetric detection, the strips of PVA wipe were placed into 30 : 70 acetonitrile : water. An aliquot was treated with N-(1-naphthyl)ethylenediamine in 0.1 m sulfuric acid. After color development, the LOD is approximately 48 microg per sample. These methods have been field tested in a hospital.

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Section: Methods For Opa In Airmentioning

confidence: 99%

Section: Methods For Opa In Airmentioning

confidence: 99%

Determination of ortho-phthalaldehyde in air and on surfaces

Tucker

2008

J. Environ. Monit.

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“…This method employs a midget impinger containing a DMSO solution of N -acetyl- l -cysteine and ethylenediamine for air sampling. The basis for this method relates to Roth’s fluorimetric method for determining analytes bearing a primary amino group 17,18 and is the same basis for the determination of OPA on surfaces. 14 Under basic conditions, the fluorigenic reagents, OPA, a thiol, and a species containing a primary amino group, undergo reaction to form a thio- N -alkyl-substituted isoindole.…”

Section: Introductionmentioning

confidence: 99%

Development, evaluation and comparison of two independent sampling and analytical methods for ortho-phthalaldehyde vapors and condensation aerosols in air

Tucker

2014

Anal. Methods

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Two independent sampling and analytical methods for ortho-phthalaldehyde (OPA) in air have been developed, evaluated and compared (1) a reagent-coated solid sorbent HPLC-UV method and (2) an impinger-fluorescence method. In the first method, air sampling is conducted at 1.0 L min−1 with a sampler containing 350 mg of silica gel coated with 1 mg of acidified 2,4-dinitrophenylhydrazine (DNPH). After sampling, excess DNPH in ethyl acetate is added to the sampler prior to storage for 68 hours. The OPA-DNPH derivative is eluted with 4.0 mL of dimethyl sulfoxide (DMSO) for measurement by HPLC with a UV detector set at 3S5 nm. The estimated detection limit is 0.016 µg per sample or 0.067 µg m−3 (0.012 ppb) for a 240 L air sample. Recoveries of vapor spikes at levels of 1.2 to 6.2 µg were 96 to 101%. Recoveries of spikes as mixtures of vapor and condensation aerosols were 97 to 100%. In the second method, air sampling is conducted at 1.0 L mm−1 with a midget impinger containing 10 mL of DMSO solution containing N-acetyl-l-cysteine and ethylenediamine. The fluorescence reading is taken 80 min after the completion of air sampling. Since the time of taking the fluorescence reading is critical, the reading is taken with a portable fluorometer. The estimated detection limit is 0.024 µg per sample or 0.1 µg m−3 (0.018 ppb) for a 240 L air sample. Recoveries of OPA vapor spikes at levels of 1.4 to 5.0 µg per sample were 97 to 105%. Recoveries of spikes as mixtures of vapors and condensation aerosols were 95 to 99%. The collection efficiency for a mixture of vapor and condensation aerosol was 99.4%. The two methods were compared side-by-side in a generation system constructed for producing controlled atmospheres of OPA vapor in air. Average air concentrations of OPA vapor found by both methods agreed within ±10%.

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“…It changes its color from yellow to orange and in the mean time decreases its fluorescence emission as a function of the amine concentration in aqueous solution. The sensitivity of the proposed dye is in the range achieved with the other well‐known reaction between primary amines, o ‐phthalic dialdehyde (OPA), and β‐mercaptoethanol in solution, which offers great sensitivity and selectivity 30–34. The necessity to use a multicomponent mixture for the analysis and the limitation for monitoring of the primary amino compounds in solution by OPA method have been overcome.…”

Section: Discussionmentioning

confidence: 99%

Optical sensor for aliphatic amines based on the simultaneous colorimetric and fluorescence responses of smart textile

Staneva

Betcheva

Chovelon

2007

J of Applied Polymer Sci

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ABSTRACT:The pH indicator proposed is a water-soluble dye based on benzo [de]antracen-7-one. Dissolving in buffered aqueous solution, it changes color from yellow to orange and in the mean time decreases its fluorescence emission as a function of the amine concentration. Viscose fabric, dyed with the same dye has been investigated as a new reversible colorimetric and fluorescent sensor material for ammonia and aliphatic amines in buffered solution. The high value of the dye pK a and the influence of the textile matrix on the selective detection of dimethylamine compared to ammonia, trimethylamine, and metylamine have been studied. The different response of the solution and textile matrix as well as the optical analyses both as a color change and a fluorescence emission have been discussed. The advantage of this sensor is the fact that depending on the analytical problem to be solved and the available instrumentation it can be used either as a fluorogenic or as a chromogenic chemosensor. In addition, the textile sensor is characterized by facile fabrication, low cost, sensibility, and reproducibility.

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Update and Evaluation of the Effectiveness of Different Thiols and Micellar Media in Roth's Fluorimetric Method for the Determination of Primary Amino Compounds

Cited by 20 publications

References 154 publications

Determination of ortho-phthalaldehyde in air and on surfaces

Determination of ortho-phthalaldehyde in air and on surfaces

Development, evaluation and comparison of two independent sampling and analytical methods for ortho-phthalaldehyde vapors and condensation aerosols in air

Optical sensor for aliphatic amines based on the simultaneous colorimetric and fluorescence responses of smart textile

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