Determination of inorganic anions, carboxylic acids and amino acids in plant matrices by capillary zone electrophoresis

Abstract

SummaryPhysiological investigations of solute transport in plants affords knowledge of solute distribution between different tissues. Capillary electrophoresis using indirect UV and laser induced fluorescence (LIF) detection is demonstrated as a useful technique for the simultaneous determination of inorganic anions, amino acids and carboxylic acids in plant samples. Cell sap obtained from plant tissues as well as simple ethanolic or aqueous plant extracts can be analysed directly without any pretreatment. This matrix stability and the very small volumes required allow fast determinations of various compounds in small plant tissue sections. In the case of carboxylic acids, resolution can be optimized using calcium for selective complexation of some of the compounds. Selective and sensitive determination of amino acids is possible using precolumn derivatisation with orthophthaldialdehyde (OPA) and laser induced fluorescence detection.

Different pathways for copper sulphate and copper nitrate antioxidation and organic acid excretion inTypha latifolia?

“…The organic acids were analysed using capillary electrophoresis with a salicylate electrolyte (Bazzanella et al, 1997). The capillary electrophoresis (CE) system G1600A (Agilent, Böblingen, Germany) equipped with a built-in diode-array detector proceeded according to Blossfeld et al (2011).…”

Dynamics of organic acid occurrence under flooding stress in the rhizosphere of three plant species from the water fluctuation zone of the Three Gorges Reservoir, P.R. China

“…It was used for the determination of organic acids with a salicylate electrolyte (Bazzanella et al 1997). The system G1600A (Agilent, Böblingen, Germany) was used, including a diode array detector and using fused silica capillaries (Polymicro, Phoenix, USA, 75 μm inner diameter, 64.5 cm total length, 56 cm effective length).…”

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

“…[266] and [250]). For example, inorganic anions including NO 2 − and NO 3 − can be efficiently separated in basal electrolytes with addition of chromate [267], pyromellitic acid [268] and salicylate [269]. Other useful probes for simultaneous separation of a range of inorganic anions and organic acids include p-aminobenzoic acid [270], phthalic acid [271], 5-sulfosalicylic acid [272], trimellitic acid (TMA) [273,274], and 1,3,5-benzenetricarboxylic acid [275].…”