Separation of petroleum hydrocarbons by selective adsorption with Sephadex LH-20

The sub‐zero temperature chromatographic isolation of transient intermediates of a multi‐step cycle: Preparation of the substrate‐free oxy‐ferrous cytochrome P450

“…However, the elution volume of camphor is 20 ml, either in pure aqueous buffer or in a mixed solvent at -20°C. These results suggest that camphor, like numerous other compounds [6] including proflavine [1 ] is retained abnormally on the LH 20 Sephadex gel. The retention is apparently not due to the same .interaction that occurs in the case of profiavine since no temperature nor solvent effects were recorded.…”

Preparative gel permeation chromatographic separation of solvent refined coal

“…This material has the property of swelling in many polar organic solvents and hence would be applicable to the separation of SRC in THF and CHC13 (9). Beside the predicted size separation phenomenon associated with Sephadex LH-20, some special affinities have also been observed in work with this material in organic solvents (24,25). Thus, materials having polar-type functions might be retained longer on the column than would otherwise be expected.…”

Petroleum

“…Gel permeation chromatography was applied to separation of hydrocarbons by Edstrom and Petro (371) and by Mair et al (871). The former authors determined elution behavior of more than a hundred polycyclic aromatics.…”

“…Results suggest that gel permeation separations are a complex function of molecular size, shape, and polarity. Mair et al (871) found that gel permeation can be used effectively to separate paraffins from cycloparaffins and alkylbenzenes from cyclanobenzenes in the 19 to 21 carbon atom range.…”