Petroleum

Tuemmler, Frederick D.; al., et

doi:10.1021/ac60274a005

Cited by 6 publications

(5 citation statements)

References 42 publications

(43 reference statements)

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“…For AcS samples, the concentration of EVs was too low, hence a larger sample volume was used. The images of AcS extracted EVs are comparable to particles observed in other publications 20 where the same difference in size was also observed.…”

Section: B Tem Imaging Of Evssupporting

confidence: 87%

“…Previous publications have evaluated new technologies of extraction 19,20 , extraction from different media 32 , or compared preanalytical parameters for EV collection and storage 43,44 . We provide here a comparative study that evaluates the sample process flow, sample nature and composition, extraction protocols and methods, to identify important parameters separately as well within a process flow and identifying co-dependencies and optimal process flows.…”

Section: Protein Expression Characterization Of Extracted Evsmentioning

confidence: 99%

“…New solutions have been developed on-chip, based on filtration 15 , acoustic 16 , viscosity 17 or thermophoresis 18 . The need for simplified extraction is served by instruments that automate the workflow, such as the centrifugal dual-filtration system called Exodisc 19 or the microbead-assisted acoustic trapping of EVs named AcouTrap 20,21 .…”

mentioning

confidence: 99%

“…In this manuscript, using cell supernatant and plasma as samples, we evaluate four label-free EV enrichment methods: ultracentrifugation (UC), size exclusion chromatography (SEC) columns, and two emerging microfluidic technologies: centrifugal filtration on a Lab-on-a-disc (LoDF) 19 and acoustic enrichment (AcS) 20 . EVs extracted from cell supernatant human squamous carcinoma cell line (A431) and pooled plasma samples were collected.…”

mentioning

confidence: 99%

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Effect of sample preprocessing and extraction methods on the physical and molecular profiles of extracellular vesicles

Alexandre

Shen

Araujo

et al. 2023

Preprint

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Extracellular vesicles (EVs) are nanometric lipid vesicles that shuttle cargo between cells. Their analysis could shed light on health and disease conditions, but EVs must first be preserved, extracted and often pre-concentrated. Here we firstly compare plasma preservation agents, and secondly, using both plasma and cell supernatant, four EV-extraction methods including (i) ultracentrifugation (UC), (ii) size exclusion chromatography (SEC), (iii) centrifugal filtration (LoDF), and (iv) accousto-sorting (AcS). We benchmarked them by characterizing integrity, size-distribution, concentration, purity and the expression profiles for nine proteins of EVs, as well as overall throughput, time-to-result and cost. We found that the difference between EDTA and citrate anticoagulants vary with the extraction method. In our hands, ultracentrifugation produced a high yield of EVs with low contamination; SEC is low-cost, fast, and easy to implement, but the purity of EVs is lower; LoDF and AcS are both compatible with process automation, small volume requirement, and rapid processing times. When using plasma, the LoDF was susceptible to clogging and sample contamination, while the AcS featured high purity but a lower yield of extraction. Analysis of protein profiles suggest that extraction methods extract different sub-population of EVs. Our study highlights the strength and weakness of sample preprocessing methods, and the variability in concentration, purity, and EV expression profiles of the extracted EVs. Pre-analytical parameters such as collection or pre-processing protocols must be considered as part of the entire process in order to address EV diversity and their use as clinically actionable indicators.

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Section: B Tem Imaging Of Evssupporting

confidence: 87%

Section: Protein Expression Characterization Of Extracted Evsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of sample preprocessing and extraction methods on the physical and molecular profiles of extracellular vesicles

Alexandre

Shen

Araujo

et al. 2023

Preprint

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“…Each line is caused by the ablation by 200 pump pulses, the grating pattern by the automatic movement of the target. This will also be useful to reach even higher repetition rates as proposed in [20]. …”

Section: High Repetition Rate Capabilitymentioning

confidence: 99%

Progress in the applicability of plasma X-ray lasers

et al. 2010

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Proposed as satellite-based weapons during the 1980s, X-ray lasing was for a long time only achieved with enormous amounts of pump energy in either nuclear explosions or at kilojoule-class laser installations. During the last few years a tremendous development was achieved, most visible in the realisation of the FEL lasers at DESY and SLAC. As important for a wider applicability is the enormous reduction in pump energy for laser pumped plasma X-ray lasers, which now brings such devices into the range of applications for diagnostics and spectroscopy even in smaller laboratories. Main developments were the transient excitation scheme and the optimized pumping concepts. This paper concentrates on developments at the GSI Helmholtzcenter at Darmstadt aiming towards reliable X-ray laser sources in the range from 50 to several 100 eV. The main driving forces for the laser development at GSI are the possible application for the spectroscopy of Li-like ions in the storage ring ESR and the future storage ring NESR at FAIR, and the interest in novel plasma diagnostics.

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