Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor

Johnson, Roy B.; Libby, R

doi:10.1128/jcm.12.3.451-454.1980

Cited by 58 publications

(16 citation statements)

References 15 publications

(8 reference statements)

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“…Human hybridoma IgA with specificity for the polyriboseribitol capsule of Haemophilus influenzae type b was a gift from Penelope Shackelford and Dan Granoff. IgG was purified from human serum by the method of Johnson and Libby (21).…”

Section: Methodsmentioning

confidence: 99%

Opsonic effect of jacalin and human immunoglobulin A on type II group B streptococci

1990

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This study examined the effect of immunoglobulin A (IgA) and the IgA-binding lectin jacalin on the phagocytosis of type II group B streptococci (GBS). Strains possessing the trypsin-sensitive and trypsinresistant components of the c protein (II/c) and type II GBS lacking the c protein (II) were examined by radiolabeled bacterial uptake, bactericidal assays, and electron microscopy. Type II/c GBS resisted phagocytosis by monocytes (4.9% ± 0.8% uptake, mean + SE, n = 25) compared with type II GBS (8.5% ± 1.4% uptake, n = 14, P = 0.03). Phagocytic killing by polymorphonuclear leukocytes was also less for the type II/c strain 78-471 than for the type II strain 79-176 (68% ± 5% versus 86% ± 4% reduction in CFU at 45 min, P = 0.03). IgA binding did not explain the resistance of type II/c GBS to phagocytosis. The uptake of type II/c GBS was not significantly different after opsonization in cord sera lacking endogenous IgA (5.93% ± 1.4%) than in the same cord sera after addition of exogenous IgA (5.48% ± 1.4%, P = 0.69, n = 9). Attempts to remove serum IgA with the IgA-binding lectin jacalin resulted in the binding of IgA-jacalin complexes to 1I/c GBS. This combination of nonspecific IgA and jacalin increased uptake of II/c GBS from 4.9% ± 0.8% to 11.8% ± 1.9% (P = 0.002). Jacalin also combined with specific, immune, monoclonal IgA bound to the surface of Haemophilus influenzae and promoted the uptake of these bacteria. Jacalin and IgA mediated phagocytosis of II/c GBS via receptors that were not dependent on divalent cations and that were not modulated by plating monocytes on antigen-antibody complexes.

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Section: Methodsmentioning

confidence: 99%

Opsonic effect of jacalin and human immunoglobulin A on type II group B streptococci

1990

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“…They were also screened for presence of immunoglobulins and other plasma proteins by double radial immunodiffusion using specific antisera against heavy chains of immunoglobulins G, A, M, and D and light chains (kappa and lambda), as well as antisera directed to whole immunoglobulin (heavy-and light-chain-specific) and polyvalent antiserum against normal human serum proteins (Kallestad Laboratories, Austin, TX). IgM and IgG fractions of patient's serum were prepared by gel filtration using Sephacryl S300 (Pharmacia, Sweden) and ion-exchange chromatography employing QAE-Sephadex A-50 [8,9]. Individual fractions were dialyzed against water and lyophilized.…”

Section: Methodsmentioning

confidence: 99%

In vitro neutrophil‐erythrocyte rosette formation mediated by a serum factor (IgG)

et al. 1984

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In vitro neutrophil-erythrocyte rosette (NER) formation occurred in the peripheral blood of an elderly man. This caused problems in cross-matching for blood transfusion initially but was resolved by performing crossmatches at 37 degrees C because this phenomenon was temperature-dependent. NER formation was independent of complement and of the type of anticoagulant used. NERs were induced using normal control cells with the patient's plasma, serum, and the IgG fraction of serum. The rosetting factor was adsorbed by heterologous group-specific erythrocytes, but not by leukocytes. No neutrophil antibodies were identified.

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“…However, in all assays with the antigens as solid-phase which detect IgM antibodies by anti-human IgM immunoglobulins, IgM-class rheumatoid factor (RF) is potentially capable of causing a falsely positive IgM result. This has been demonstrated in indirect immunoassays and IF tests for the detection of antibodies to Treponema pallidum (9), Toxoplasma gondii (1,6,11,12) and the following viruses: rubella (5,8,13,14), cytomegalo- (13,15), measles (14,16), Epstein-Barr (4), influenza (13), herpes simplex (14,15,16) and mumps (14). For the RF interference the following mechanism is supposed: Antigen-specific IgG first attaches to the solid-phase bound antigen, IgM-RF reacts with the altered Fc fragment of the bound IgG antibodies and the labelled antibodies to human IgM recognize the RF molecules (13,15,16).…”

Section: Introductionmentioning

confidence: 98%

Does IgM‐Class Rheumatoid Factor Disturb Candida‐Specific IgM‐diagnostics?

Schrenker¹,

H²,

Huschka³

1984

Mycoses

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Summary: The sera of 35 patients with positive rheumatoid factor were examined by an IgM Candida immunofluorescence test. No significant difference was found between the IgM Candida antibody titres before and after elimination of the expected rheumatoid factor interference using ion‐exchange chromatography of the sera. Consequently, IgM‐class rheumatoid factor interference in the IgM Candida immunofluorescence test was not demonstrated, even when high rheumatoid factor titres, shown by latex‐agglutination, and IgG Candida antibody titres were present. Zusammenfassung: Die Rheumafaktor‐positiven Seren von 35 Patienten wurden mit einem IgM‐Candida‐Immunfluoreszenz‐Test geprüft. Es fanden sich keine signifikanten Differenzen zwischen den IgM‐Candida‐Antikörpertitern vor und nach Elimination der erwarteten Rheumafaktorinterferenz durch Ionenaustausch‐Chromatographie der Seren. Folglich wurde eine Störung durch den Rheumafaktor der IgM‐Klasse im Candida‐Immunfluoreszenztest nicht nachgewiesen, auch nicht bei hohen Rheumafaktortitern im Latex‐Agglutinationstest und IgG‐Candida‐Antikörpertitem.

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Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor

Cited by 58 publications

References 15 publications

Opsonic effect of jacalin and human immunoglobulin A on type II group B streptococci

Opsonic effect of jacalin and human immunoglobulin A on type II group B streptococci

In vitro neutrophil‐erythrocyte rosette formation mediated by a serum factor (IgG)

Does IgM‐Class Rheumatoid Factor Disturb Candida‐Specific IgM‐diagnostics?

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