Separation of constituents from Bergenia stracheyi (Hook. F. & Thoms.) Engl. by high‐speed countercurrent chromatography with elution mode and its antidiabetic and antioxidant in vitro evaluation

Abstract

Diabetes, a metabolic disorder, is caused by a high blood sugar level. Diabetes is an increasing health issue and search for potent antidiabetic agents is desirable. Owing to its ethnomedicinal value, the Himalayan perennial herb Bergenia stracheyi (Hook. f. & Thoms.) Engl. (Saxifragaceae Juss) is used to treat diabetes. Herein, an efficient high‐speed countercurrent chromatography with elution mode is reported for separation of active compounds from B. stracheyi. In current investigation, six main compounds including β‐arbutin (1), bergenin (2), 6‐O‐galloylarbutin (3), gallic acid (4), 11‐O‐galloylbergenin (5), and (‐)‐epicatechin 3‐O‐gallate (6) with above 95% purity were efficiently separated in a single run using biphasic tert‐butyl methyl ether/n‐butanol/methanol/water (1:3:1:5, v/v/v/v) solvent system. The structures of these compounds were characterized using spectral techniques and compared with the literature. Antidiabetic and antioxidant activities evaluation of the study samples showed that β‐arbutin (1) and 6‐O‐galloylarbutin (3) have a significant protective effect, especially at high dose against hydrogen peroxide induced oxidative injury. Our results might help further in‐depth phytochemical and biological evaluation studies in search of potent antidiabetic compounds from B. stracheyi.

Development of a method to screen and isolate xanthine oxidase inhibitors from black bean in a single step: Hyphenation of semipreparative liquid chromatography and stepwise flow rate countercurrent chromatography

“…Next, the phases (0.5 mL) were added to other test tubes, followed by drying-dissolving them in 1.0 mL of chromatographygrade methanol and HPLC analysis. The K value for each sample was calculated by dividing the peak area of the upper phase by that of the lower phase (K = A U /A L ) [18]. Our results indicated that the ethyl acetate-n-butyl alcohol-methanol-water (6.0:2.0:1.0:10.0, v/v/v/v) system was the optimal solvent system in this study.…”

Arbutin: Occurrence in Plants, and Its Potential as an Anticancer Agent

“…Certain plants from families like Fabaceae [5,72,74,86], Lamiaceae [87,128,141] and Plantaginaceae [132,154,160] are also notable sources of this hydroquinone glycoside (Table 1). At least three genera of each of the families Rutaceae [48,94,161] and Saxifragaceae [22,36,131] are known to produce arbutin (Table 1). While leaves are the main source of the compound, it is present in other plant parts, e.g., aerial parts, flowers, fruits, stem and twigs (Table 1).…”

An efficient isolation and purification of broad partition coefficient range ginsenosides from roots of Panax quinquefolium L. by linear gradient counter‐current chromatography coupled with preparative high‐performance liquid chromatography

“…As a rapidly-growing approach for natural product isolation exploiting the principle of liquid-liquid partition, high-speed counter-current chromatography (HSCCC) has been applied to preparative isolating and purifying diverse natural products [21][22][23]. Besides, HSCCC has several superiorities over the solid-phase chromatographic and classic liquid-liquid extraction strategies, including the absence of irreversible adsorption, high capacity, high sample recovery, low possibility of sample denaturation, as well as small solvent usage [24]. Diverse modes of HSCCC are available at present, including normal phase [25], reverse phase [26], recycling mode [26], dual mode [27], pH-zone refining [28], and gradient mode [29], allowing its broad application for separating and purifying natural productderived active constituents.…”