Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction

Lim, Gio Bin; Lee, Sang-Yun; Lee, Eun Kyu; Haam, Seok Jin; Kim, Woo Sik

doi:10.1016/s1369-703x(02)00023-2

Cited by 125 publications

(58 citation statements)

References 10 publications

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“…Supercritical fluid chromatography has been used to recover astaxanthin (Lim et al, 2002), polyunsaturated fatty acids and other compounds. Some other chromatographic methods that have been used for recovering pure fatty acids (or equivalent esters) have included reverse phase chromatography, silica gel adsorption chromatography and argentated silica gel chromatography (Robles Medina et al, 1995;Belarbi et al, 2000).…”

Section: Extraction and Purification Of Metabolitesmentioning

confidence: 99%

Recovery of microalgal biomass and metabolites: process options and economics

Grima

Belarbi

Fernández

et al. 2003

Biotechnology Advances

1,853

705

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Commercial production of intracellular microalgal metabolites requires the following: (1) largescale monoseptic production of the appropriate microalgal biomass; (2) recovery of the biomass from a relatively dilute broth; (3) extraction of the metabolite from the biomass; and (4) purification of the crude extract. This review examines the options available for recovery of the biomass and the intracellular metabolites from the biomass. Economics of monoseptic production of microalgae in photobioreactors and the downstream recovery of metabolites are discussed using eicosapentaenoic acid (EPA) recovery as a representative case study. D

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Section: Extraction and Purification Of Metabolitesmentioning

confidence: 99%

Recovery of microalgal biomass and metabolites: process options and economics

Grima

Belarbi

Fernández

et al. 2003

Biotechnology Advances

1,853

705

View full text Add to dashboard Cite

show abstract

“…In addition, the use of toxic solvents can be avoided, since carbon dioxide CO 2 is inexpensive and generally recognized as a safe GRAS solvent, which is easy to separate from the extract 14 16 . Supercritical fluids have outstanding extractive properties such as liquid-like density, high compressibility, high diffusivity, and low viscosity 17 .…”

Section: Introductionmentioning

confidence: 99%

“…The extraction efficiency could be improved by fine-tuning the solvent selectivity, and the solubility of the carotenoids in the mixture could be increased by introducing different types and concentrations of cosolvents entrainers to the SC-CO 2 . In addition, the relatively low solubility of astaxanthin in SC-CO 2 requires the addition of polar modifiers or high pressures 40 MPa to improve its dissolution in the mixture with the simultaneous enhancement in extraction yields 17,18,22,23 .…”

Section: Introductionmentioning

confidence: 99%

Co-solvent Selection for Supercritical Fluid Extraction of Astaxanthin and Other Carotenoids from Penaeus monodon Waste

et al. 2014

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“…About 130 tons of astaxanthin is required annually to feed the salmonids produced globally by aquaculture (Bjerkeng, 2008). At present, more than 95% of the astaxanthin used worldwide is chemically synthesized, and has a lower antioxidant activity compared with natural astaxanthin (Lim et al, 2002). Therefore, the production of natural astaxanthin from mass cultures of astaxanthin-rich Haematococcus pluvialis is becoming increasingly important in the microalgalbiotechnology industry.…”

Section: Introductionmentioning

confidence: 99%

Stability and changes in astaxanthin ester composition from Haematococcus pluvialis during storage

Miao

Geng

et al. 2013

Chin. J. Ocean. Limnol.

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In this paper, we investigated the effects of temperature, oxygen, antioxidants, and corn germ oil on the stability of astaxanthin from Haematococcus pluvialis under different storage conditions, and changes in the composition of astaxanthin esters during storage using high performance liquid chromatography and spectrophotometry. Oxygen and high temperatures (22-25°C) signifi cantly reduced the stability of astaxanthin esters. Corn germ oil and antioxidants (ascorbic acid and vitamin E) failed to protect astaxanthin from oxidation, and actually signifi cantly increased the instability of astaxanthin. A change in the relative composition of astaxanthin esters was observed after 96 weeks of long-term storage. During storage, the relative amounts of free astaxanthin and astaxanthin monoesters declined, while the relative amount of astaxanthin diesters increased. Thus, the ratio of astaxanthin diester to monoester increased, and this ratio could be used to indicate if astaxanthin esters have been properly preserved. If the ratio is greater than 0.2, it suggests that the decrease in astaxanthin content could be higher than 20%. Our results show that storing algal powder from H . pluvialis or other natural astaxanthin products under vacuum and in the dark below 4°C is the most economical and applicable storage method for the large-scale production of astaxanthin from H . pluvialis . This storage method can produce an astaxanthin preservation rate of at least 80% after 96 weeks of storage.

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Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction

Cited by 125 publications

References 10 publications

Recovery of microalgal biomass and metabolites: process options and economics

Recovery of microalgal biomass and metabolites: process options and economics

Co-solvent Selection for Supercritical Fluid Extraction of Astaxanthin and Other Carotenoids from Penaeus monodon Waste

Stability and changes in astaxanthin ester composition from Haematococcus pluvialis during storage

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