Separation of amino acids, peptides and corresponding Amadori compounds on a silica column at elevated temperature

Hao, Zhigang; Lu, Chih-Ying Joey; Xiao, Baiming; Weng, Naidong; Parker, Barry; Knapp, Michael; Ho, Chi‐Tang

doi:10.1016/j.chroma.2007.02.057

Cited by 61 publications

(38 citation statements)

References 30 publications

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“…(The bottom panel depicts unpublished data from our Laboratories related to ref. [21]). vated temperature).…”

Section: Column Temperature Impact On Hilic Selectivitymentioning

confidence: 93%

“…Column temperature was at 808C for (top, left), 308C for (top, right) and 58C for bottom. Adapted from Hao et al [21] with permission.…”

Section: Column Temperature Impact On Hilic Selectivitymentioning

confidence: 96%

“…The van't Hoff plots for G, DG, TG, GG, GDG and GTG under HILIC condition of Hypersil silica column, 10061 mm, particle size of 3 lm with a flow rate of 100 lL/min, the column temperature varying from 5 to 808C, mobile phase for top: water/ACN (25:75, v/v) containing 0.4% formic acid and mobile phase for bottom: water/methanol (2:98, v/v) containing 0.4% formic acid. Adapted from Hao et al [21] with permission. (The bottom panel depicts unpublished data from our Laboratories related to ref.…”

Section: Column Temperature Impact On Hilic Selectivitymentioning

confidence: 96%

“…If an amine (NH 2 ) column is applied, only the anion (COO -) group can provide an extra ion-exchange retention power on the column. When multiple polar protic functional groups such as -OH, -NH (1l2) and -SH contribute to analyte polarity, hydrogen-bonding retention mechanism will exhibit its strength in most HILIC columns [19,20] and this strong hydrogen bond capability can be weakened by switching ACN to methanol in the mobile phase [21]. If greater attention is paid to classification of polar ingredients we need to analyze, this multimodal retention mechanism will benefit our analysts for method development design, especially in column and mobile phase selections.…”

Section: Introductionmentioning

confidence: 99%

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Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Hao¹,

Xiao

Weng

2008

J of Separation Science

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244

152

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The retention mechanism and chromatographic behavior for different polar analytes under hydrophilic interaction chromatography (HILIC) conditions have been studied by application of different mobile phases and stationary phases to various analytes at different temperatures. In addition to the commonly accepted mechanism of analyte liquid-liquid partitioning between mobile phase and water-enriched solvent layer which is partially immobilized onto the surface of the stationary phase, hydrogen-bonding, hydrophobic interaction, and ion-exchange interactions may also be involved. The predominant retention mechanism in HILIC separation is not always easily predictable. It can depend not only on the characteristics of the analytes but also on the selection of mobile and stationary phase compositions. The objective of this review is to evaluate the potential application of column temperature and mobile phase composition toward improving HILIC selectivity. The functional groups from analyte structures, stationary phase materials and organic mobile phase solvents will be highlighted.

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“…(The bottom panel depicts unpublished data from our Laboratories related to ref. [21]). vated temperature).…”

Section: Column Temperature Impact On Hilic Selectivitymentioning

confidence: 93%

“…Column temperature was at 808C for (top, left), 308C for (top, right) and 58C for bottom. Adapted from Hao et al [21] with permission.…”

Section: Column Temperature Impact On Hilic Selectivitymentioning

confidence: 96%

Section: Column Temperature Impact On Hilic Selectivitymentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Hao¹,

Xiao

Weng

2008

J of Separation Science

Self Cite

244

152

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“…Column temperature is also an important parameter that affects the retention and separation of analytes in HILIC [11] [12]. The effect of column temperature was studied using the Van't Hoff equation, which is often used to describe the relationship between the capacity factor (k') and column temperature (T): …”

Section: Resultsmentioning

confidence: 99%

A HILIC Mechanism Discussion for the Retention of HP-<i>β</i>-CD

Wei¹,

Wang²,

Wu³

2014

OALib

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A hydrophilic interaction liquid chromatography (HILIC) method coupled with a refractive index (RI) detector was developed for the determination of hydroxypropyl-β-cyclodextrin (HP-β-CD). This HILIC method used simple acetonitrile/water (80/20, v/v) as the mobile phase to provide sufficient retention for HP-β-CD. The method was found to be specific without interference from the other constituents in pharmaceutics. The method was also validated for linearity and accuracy of the drug sample. The effects of various parameters, such as acetonitrile content, mobile phase pH, and column temperature on HILIC analysis were investigated. Furthermore, the retention mechanism was assessed by the evaluation of the common chromatographic parameters.

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