Separation, identification and rapid determination of liensine, isoliensinine and neferine from embryo of the seed of Nelumbo nucifera GAERTN. by liquid chromatography coupled to diode array detector and tandem mass spectrometry

Chen, Yi; Fan, Guorong; Wu, Huiling; Wu, Yutian; Mitchell, Annabel

doi:10.1016/j.jpba.2006.06.016

Cited by 84 publications

(68 citation statements)

References 26 publications

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“…And in this study, the correlation coefficient of regression equation with IS was greater than 0.999 for all analytes, which was also better than that of reported in Ref. [13]. Furthermore, the IS method adopted in this study is fast and convenient.…”

Section: Linearity Repeatability and Detection Limitcontrasting

confidence: 41%

“…(The LODs in this study is higher than that of reported in Ref. [13].) Further investigation will be focused on the sensitivity improvement.…”

Section: Linearity Repeatability and Detection Limitmentioning

confidence: 56%

“…Comparing Ref. [13] that also used IS, the IS method established in our study also shows superiority in the RSDs of the intrarun (interrun) precision. [The data in reference were 1.8-6.1% (2.7-7.8%), 1.9-8.9% (2.3-12.7%) and 1.7-10.3% (2.0-7.1%) for LIE, NEF and ISO, respectively.]…”

Section: Linearity Repeatability and Detection Limitmentioning

confidence: 60%

“…Since the first isolation of LIE in 1962 [6], the three alkaloids have received considerable attention and many studies were focused on them [10][11][12][13]. However, up to now, to our best knowledge, there is no report on the separation and determination of the three analytes using capillary electrophoresis (CE).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Micellar Electrokinetic Chromatography Using a Cationic Surfactant for Rapid Separation and Determination of Bisbenzylisoquinoline Alkaloids from Embryo of the Seed of Nelumbo nucifera Gaertn

Zhu

Zhang

et al. 2011

Chromatographia

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Liensinine (LIE), isoliensinine (ISO) and neferine (NEF) from embryo of the seed of Nelumbo nucifera Gaertn were first separated and quantitatively analyzed by micellar electrokinetic chromatography with internal standard (IS) using a cationic surfactant, tetradecyltrimethyl ammonium bromide (TTAB). Complete separation of the analytes was optimally achieved within 15 min using 40 mM sodium dihydrogenphosphate (pH 6.40) containing 10 mM TTAB and 15% (v/v) methanol as buffer. Tetrahydropalmatine was used as IS to improve the repeatability and linearity relativity. The IS method resulted in excellent linearity, with correlation coefficients of regression equations of 0.9997, 0.9997 and 0.9992 for LIE, ISO and NEF, respectively. Finally, two sample extraction methods were used to investigate the contents of different parts of the embryos.

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Section: Linearity Repeatability and Detection Limitcontrasting

confidence: 41%

“…(The LODs in this study is higher than that of reported in Ref. [13].) Further investigation will be focused on the sensitivity improvement.…”

Section: Linearity Repeatability and Detection Limitmentioning

confidence: 56%

Section: Linearity Repeatability and Detection Limitmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Micellar Electrokinetic Chromatography Using a Cationic Surfactant for Rapid Separation and Determination of Bisbenzylisoquinoline Alkaloids from Embryo of the Seed of Nelumbo nucifera Gaertn

Zhu

Zhang

et al. 2011

Chromatographia

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show abstract

“…Chen et al developed an LC-MS/MS method for high throughput simultaneous measurement of liensinine, isoliensinine, and neferine in extracts of Nelumbo nucifera [172]. Using MRM mode, liensinine, isoliensinine and neferine were monitored at the ion transitions at m/z 611-206, 611-192, 625-206, respectively.…”

Section: Alkaloidsmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

162

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An Updated Review on Nelumbo Nucifera Gaertn: Chemical Composition, Nutritional Value and Pharmacological Activities

Sahu,

Sahu

et al. 2024

Chemistry & Biodiversity

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Nelumbo nucifera Gaertn is a recognised herbal plant in ancient medical sciences. Each portion of the plant leaf, flower, seed and rhizome is utilised for nutritional and medicinal purposes. The chemical compositions like phenol, alkaloids, glycoside, terpenoids and steroids have been isolated. The plant contains various nutritional values like lipids, proteins, amino acids, minerals, carbohydrates, and fatty acids. Traditional medicine confirms that the phytochemicals of plants give significant benefits to the treatment of various diseases such as leukoderma, smallpox, dysentery, haematemesis, coughing, haemorrhage, metrorrhagia, haematuria, fever, hyperlipidaemia, cholera, hepatopathy and hyperdipsia. To verify the traditional claims, researchers have conducted scientific biological in vivo and in vitro screenings, which have exhibited that the plant keeps various notable pharmacological activities such as anticancer, hepatoprotective, antioxidant, antiviral, hypolipidemic, anti‐obesity, antipyretic, hypoglycaemic, antifungal, anti‐inflammatory and antibacterial activities. This review, summaries the nutritional composition, chemical constituents and biological activities substantiated by the researchers done in vivo and in vitro.

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Separation, identification and rapid determination of liensine, isoliensinine and neferine from embryo of the seed of Nelumbo nucifera GAERTN. by liquid chromatography coupled to diode array detector and tandem mass spectrometry

Cited by 84 publications

References 26 publications

Micellar Electrokinetic Chromatography Using a Cationic Surfactant for Rapid Separation and Determination of Bisbenzylisoquinoline Alkaloids from Embryo of the Seed of Nelumbo nucifera Gaertn

Micellar Electrokinetic Chromatography Using a Cationic Surfactant for Rapid Separation and Determination of Bisbenzylisoquinoline Alkaloids from Embryo of the Seed of Nelumbo nucifera Gaertn

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

An Updated Review on Nelumbo Nucifera Gaertn: Chemical Composition, Nutritional Value and Pharmacological Activities

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