Separation and quantification of silver nanoparticles and silver ions using reversed phase high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry in combination with isotope dilution analysis

ICP-MS for the analysis at the nanoscale – a tutorial review

“…212 The developed method was applied for separation of the NIST Au nanoparticles of 10 nm, 30 nm and 60 nm (see Table 1). Baseline separation was not achieved, but in particular the separation of the 60 nm particles from the 30 nm particles was much better compared to RPC 201 , SEC 192 and HDC 192 . Separation speed is higher (10 minutes runtime) than for HDC (15 to 30 minutes) and for SEC (30 to 60 minutes), but lower compared to RPC (7 minutes).…”

“…Calibration by post-column isotope dilution was employed for improved Ag quantification. 201 This method was applied by Soto-Alvaredo et al for the speciation/fractionation analysis of Au-nanoparticles and low molecular weight Au metabolites in tissue samples from Wistar rats after intraperitoneal injection of 10 nm Au nanoparticles. Critical aspect was the extraction of the Au species/fractions from the tissue without changing the particle properties, aggregation and dissolution state.…”

“…Connection is achieved by a PEEK capillary from the channel or column outlet to the nebuliser inlet. Optionally a T-piece is inserted in this transfer line for introduction of an internal standard or for special calibration strategies 201,199 (see also chapter 5.1.1). However, the coupling of CE to ICP-MS is less straightforward due to the flow rates in the nL min -1 range and the need for closing the electric circuit.…”

Quantification of metals in single cells by LA-ICP-MS: comparison of single spot analysis and imaging

“…Methods to measure nanoparticles using the popular sp-ICP-MS mode were already established in Dr. Jakubowskis' group (BAM division 1.1) and served as a starting point. [77][78][79] First tests were carried out using a quadrupole ICP-MS (iCAP Q, Thermo Fisher Scientific)…”

Single‐gap Microelectrode Functionalized with Single‐walled Carbon Nanotubes and Pbzyme for the Determination of Pb2+

“…The electrochemical biosensor , known as a signal transducer, can convert tiny biological information to an easily measured electronic signal (potential/current). Biosensor‐related research has experienced explosive growth over the past two decades due to its merits including high specificity, small size, quick response and simple operation in comparison with the most common conventional methods, such as X‐ray fluorescence spectroscopy , inductively coupled plasma mass spectrometry , atomic absorption spectrometry, atomic fluorescence spectrometry , high‐performance liquid chromatography . With the development of nanomaterials and biomaterials, they provide much more opportunities to improve the sensing performance of biosensor, especially the sensitivity and selectivity for the ultra‐low concentration of the analyte.…”