Quantification of metals in single cells by LA-ICP-MS: comparison of single spot analysis and imaging

Abstract

A comparison of single cell LA-ICP-MS imaging and single spot analysis highlights the advantages of both methods.

Capabilities of automated LA-ICP-TOFMS imaging of geological samples

“…† In brief, the different dimensions of the TOFMS data are used to trigger the TOFMS data acquisition for individual pixels and accordingly store data separately and avoid part of the previous cumbersome data processing. 24,35 Since the soware prompts triggers and controls for the sample translation stage, laser, and data acquisition independently, exible stage movements and ablation modes are available. Irrespective of ablation mode and sample movement, the imaging control system enables to acquire the full mass spectrum and a transient signal for each sample position results in one pixel.…”

“…At. Spectrom., 2020, 35,[2255][2256][2257][2258][2259][2260][2261][2262][2263][2264][2265][2266] This journal is © The Royal Society of Chemistry 2020 control soware enabling a shape adapted sample ablation was developed.…”

“…30) However, while sensitivities still need to be sufficient to yield signals for individual laser pulses, smaller pixel sizes were also accomplished with overlapping scans and signal deconvolution. [31][32][33][34][35] To achieve high data acquisition speed while maintaining the lateral resolution, low dispersion ablation cells have to be used to transport the aerosol in a dense plume without pulse-topulse aerosol mixing into the ICP-MS. 36 Shorter signal durations increase the signal-to-noise ratio and allows a faster data acquisition. In recent years, signal duration of <10 ms (full width at 1% of the maximum, FW0.01M) for LA-ICP-MS imaging were achieved.…”

Isotopically Enriched Tracers and Inductively Coupled Plasma Mass Spectrometry Methodologies to Study Zinc Supplementation in Single-Cells of Retinal Pigment Epithelium in Vitro

“…For the analysis of small-sized structures, such as cells, one of the main limitations is related to the compromise required between high spatial resolution and good sensitivity. 6,13,14 LA-ICP-MS can be also employed in combination with enriched stable isotopes as tracers in biological systems. 15 In this vein, Urgast et al 16,17 reported studies using a double tracer (t), t70 Zn and t67 Zn, with rats to determine Zn kinetics at the microscale within different tissue features (heart and brain tissue sections were analyzed).…”

“…single metal chelates such as DOTA lanthanide complexes and polymeric labels such as MAXPAR®) were investigated for imaging of proteins within single cells. 14,20,23,24 Furthermore, some strategies based on standards spotted onto nitrocellulose membranes or microarray gelatin standards were used to obtain not only elemental qualitative distributions but also quantitative information from cell cultures. 14,25 The aim of our investigation was to evaluate the Zn absorption in cultured HRPEvs cells after its supplementation with isotopically-enriched tracers ( t68 Zn and t70 Zn).…”