Separation and purification of benzylpenicillin produced by fermentation using coupled ultrafiltration and nanofiltration technologies

Tessier, L.; Bouchard, P.; Rahni, Mohamed

doi:10.1016/j.jbiotec.2004.09.002

Cited by 44 publications

(21 citation statements)

References 11 publications

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“…Solvent extraction has several difficulties which can cause problems such as final product contamination, low extraction yield, clogging of equipment, and high solvent losses [1,3,4]. Therefore new separation techniques have been studied to overcome the difficulties experienced whilst using conventional solvent extraction for penicillin G. Some of the techniques that have been the subject of experimental studies include ultrafiltration and nanofiltration [3,5], liquid surfactant membranes [6], and reactive extraction [1].…”

Section: Introductionmentioning

confidence: 99%

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Penicillin G solubilisation into AOT reverse micelles

Mohd‐Setapar

Wakeman

Tarleton

2009

Chemical Engineering Research and Design

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Extraction of penicillin G from an aqueous phase into an organic phase containing AOT reverse micelles has been investigated. The extraction is influenced by the initial penicillin G concentration, the salt type and concentration in the aqueous phase, pH, and surfactant concentration. The results show that penicillin is an interfacially active compound that interacts with AOT, with the interfacial association being dependent on both pH and surfactant concentration. When the concentration ratio [P] aq /[S] is high precipitation of the penicillin occurs. The distribution coefficient favours transfer of the penicillin into the reverse micelles at moderate AOT concentrations. The distribution coefficient at infinite dilution, K  , is shown to be a function of both pH and surfactant concentration; similar trends in the value of K  were observed at different pH values; K  decreases as the surfactant concentration is increased. Reverse micelle formation affects the volume of water transferred into the organic phase; from measurements of the water transferred, the size of the reverse micelles was estimated to be about 3 nm and the number of AOT molecules per reverse micelle about 360.

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Section: Introductionmentioning

confidence: 99%

“…They are easy to scale up, have a high efficiency, can be made selective, have a low energy demand, and can be operated continuously [7,8,[10][11][12][13][14]. They can also offer moderate thermal conditions for processing of biomaterials [5,9]. The method is a kind of solvent extraction using reverse micelles as the extractant [10].…”

Section: Introductionmentioning

confidence: 99%

Penicillin G solubilisation into AOT reverse micelles

Mohd‐Setapar

Wakeman

Tarleton

2009

Chemical Engineering Research and Design

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“…One of the major problems in the production of fermentation is the concentration and purification of antibiotics from the fermented broth. 1,2 The conventional steps are: filtration (removal mycelial cells), solvent extraction (isolation and purification), and subsequent crystallization. During solvent extraction, antibiotics are extracted into an immiscible organic phase, such as n-butylacetate at certain pH value, and concentrated by vacuum distillation.…”

Section: Introductionmentioning

confidence: 99%

“…The purification cost may vary from 20 to 50% of the total production cost. 2 Membrane separation technologies have an improved efficiency and reduced operating cost in comparison with the traditional concentration processes. For the antibiotic's production of fermentation, ultrafiltration (UF) was applied to remove the emulsifying agents by some researchers on lab and pilot scale.…”

Section: Introductionmentioning

confidence: 99%

“…So, NF is fit for the direct concentration and purification of antibiotics from the ultrafiltrated broths. 1,2,4 Compared with RO, NF can provide many advantages, such as lower operating pressure, larger permeation flux, and removing inorganic ions with significant charges. 2 Benzylpenicillin (penicillin G) is a widely used antibiotics produced by fermentation.…”

Section: Introductionmentioning

confidence: 99%

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Concentration of benzylpenicillin sodium by polyimide nanofiltration membrane

Shi

Zhang

et al. 2007

J of Applied Polymer Sci

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In this work, a nanofiltration membrane prepared from soluble polyimide (HQDPA‐DMMDA) was used to concentrate benzylpenicillin sodium solutions at 10 and 14°C. Three benzylpenicillin sodium solutions with the concentration of 7.2, 13.0, and 19.7 g/L were used. The pressure varied from 0.5 to 2.0 MPa. The rejection reached 85% for the solution of 19.7 g/L under 2.0 MPa. To the solution of 7.2 g/L, the largest permeation flux was 9.5 kg/m2 h. Temperature had a slight influence on flux, but the influence on rejection was complicated. A sharp increasing phenomenon of rejection appeared under 0.5 MPa when the concentration was 19.7 g/L. This was elucidated by Martínez‐Landeira's works on benzylpenicillin's colloids. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007

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