Separation and Determination of Clenbuterol, Cimaterol and Salbutamol by Capillary Electrophoresis with Amperometric Detection

Abstract

Capillary electrophoresis with amperometric detection was applied to determine some b 2 -agonists, such as clenbuterol, cimaterol and salbutamol in this paper. The working electrode used was a 0.3 mm diameter carbon disk electrode. In this work, the pH 6.0 -6.4 borax-potassium dihydrogen phosphate was used as running buffer (150 mmol/L), 10 kV as the separation voltage and 1.05 V (vs. Ag/AgCl, 3 mol/L KCl) as the detection potential. Under the optimum conditions, the analytes were baseline separated within 16 min. Linear range for cimaterol, clenbuterol and salbutamol was 1.0 -2000, 2.0 -2000 and 1.0 -2000 ng/mL, respectively. The detection limits (define as 3s/k) were 0.5, 1.0 and 0.4 ng/mL for cimaterol, clenbuterol and salbutamol, respectively. The developed method has been applied to determine these three analytes in feed and urine by standard addition. The mean recoveries for these three analytes ranged from 89.0% to 102.0%.

Recent advances in the application of capillary electromigration methods for food analysis

“…CE methods have been successfully applied to the simultaneous determination of multiple illegal drugs in animal feeds [94,95]. In these works, 2-thiouracil and phenylthiouracil were derivatized with 5-iodoactamidofluoresceins prior to their analysis by CZE-LIF using a 20 mM disodium hydrogen phosphate at pH 10.0 [94].…”

“…In these works, 2-thiouracil and phenylthiouracil were derivatized with 5-iodoactamidofluoresceins prior to their analysis by CZE-LIF using a 20 mM disodium hydrogen phosphate at pH 10.0 [94]. Clenbuterol, cimaterio and salbutamol were analyzed by CZE-AD using a 150 mM borax-potassium dihydrogen phosphate at pH 6.0 [95].…”

Separation and determination of flavonoids using microemulsion EKC with electrochemical detection

“…The details of this system have been described in the previous works [28,29]. A prealigned electrochemical cell, consisting of threeelectrode (a 500 mm diameter carbon disk working electrode (the preparation of it has been reported by Wang [29]), a platinum auxiliary electrode and an Ag/AgCl reference electrode), was used in combination with a BAS LC-4C amperometric detector (Bioanalytical System, West Lafayette, IN, USA).…”

Monoclonal antibody-based homogeneous immunoassay for three banned agonists and molecular modeling insight

“…The World Anti-doping Agency has prohibited the oral use of SAL, and a concentration of more than 1000 ng/mL in urine is considered as an indication of doping (Pichon, Venisse, Krupka, Perault-Pochat, & Denjean, 2006). Quantitative detection of SAL currently is limited to instrumental methods, high-performance liquid chromatography (Cooper & Shepherd, 1996;Halabi, Ferrayoli, Palacio, Dabbene, & Palacios, 2004;Maudens, Zhang, & Lambert, 2004;Mazhar & Chrystyn, 2009;Murtaza, Ahmad, & Akhtar, 2009), gas chromatography-mass spectrometry (Tanaka, Yoneda, Inoue, Sugiura, & Ueno, 2000;Zhang et al, 2006), capillary electrophoresis (Chen, Wang, Duan, Chen, & Chen, 2005;Chu, Geng, Zhou, & Ye, 2007;Felix et al, 2006), etc. Most of these methods share a number of important drawbacks.…”