Separation and characterization of peanut phospholipid molecular species using high‐performance liquid chromatography and fast atom bombardment mass spectrometry

Abstract

Total lipid extracts from peanut seed were separated on a silica column into a triacylglycerol fraction and a polar lipid fraction by high-performance liquid chromatography (HPLC). The polar fraction containing the phospholipids was retained on the precolumn, and the triacylglycerol fraction was eluted to a waste flask by a special valve arrangement. Phospholipids were eluted from the precolumn and separated into various classes on a silica analytical column. Each phospholipid class was manually collected and subsequently subjected to reversed-phase HPLC in tandem with a fast atom bombardment mass spectrometer. Phosphatidylethanolamine was separated into five molecular species. Phosphatidylinositol and phosphatidylcholine were each separated into six molecular species.Paper no. J8689 in JAOCS 76, 49-56 (January 1999). FIG. 3. HPLC separation of phospholipid molecular species detected by full-scan analysis with FAB-mass spectrometry (MS). (A) PE molecular species, (B) PI molecular species, (C) PC molecular species. See Figures 1 and 2 for other abbreviations.

UV analysis of Amadori-glycated phosphatidylethanolamine in foods and biological samples

“…A reverse-phase column is often used for phospholipid analysis, especially for the separation of the molecular species of phospholipids (19,20). However, in this study we used a normal-phase column on which we could elute the molecular species as a single peak by using a gradient elution profile.…”

Comparison and Characterization of Soybean and Sunflower Lecithins Used for Chocolate Production by High-Performance Thin-Layer Chromatography with Fluorescence Detection and Electrospray Mass Spectrometry

“…3 Although lecithin is an important food additive (E 322), it also has a wide range of other application possibilities such as emulsifier in feed products, pharmaceutical products, cosmetics, and the paint industry and as an industrial lubricant. 2,3,6 In the chocolate industry, the emulsifying properties of glycerophospholipids are crucial. By reducing the interfacial tension between the continuous phase (cocoa butter) and the dispersive phase (particles such as sugars, cocoa, and milk powder), glycerophospholipids support the miscibility and stability of the product.…”

“…Commercially available lecithin products are predominantly composed of glycerophospholipids (∼50%) and triacylglycerides (∼34%), with lower amounts of glyceroglycolipids and carbohydrates as well as various minor compounds (e.g., sterols, tocopherols, and free fatty acids) . Although lecithin is an important food additive (E 322), it also has a wide range of other application possibilities such as emulsifier in feed products, pharmaceutical products, cosmetics, and the paint industry and as an industrial lubricant. ,, …”

Characterization of Molecular Properties and Expression of Gene GmPLMT and Its Effects on the Production of Lipid Metabolites in Soybean and Arabidopsis thaliana

“…In recent years, the separation, extraction, and biological functions of phospholipids have been extensively investigated. For example, studies have shown that the yields of lipids and phospholipids using subcritical dimethyl ether are higher than those based on propane and its mixed solvents, while the biological functions of the asymmetric distribution of phospholipids in cell membranes have been well explored [9][10][11]. However, studies on the functions of the gene encoding phosphatidyl methyltransferase (PLMT) at genetic level are sparsely reported in plants, whereas studies have shown that the PLMTs in animals and yeast catalyze the methylation of PtdEtn to generate PtdCho in vivo [12,13].…”