Separation and characterization of ciprofloxacin, difloxacin, lomefloxacin, norfloxacin, and ofloxacin oxidation products under potassium permanganate treatment in acidic medium by UPLC-MS/MS

Hubicka, Urszula; Żmudzki, Paweł; Żuromska-Witek, Barbara; Zajdel, Paweł; Pawłowski, Maciej; Krzek, Jan

doi:10.1016/j.talanta.2013.01.055

Cited by 44 publications

(25 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, there has been an increased demand for efficient detection methods of FQs in food products. Several types of analytical methods have been developed for the detection of FQs including UPLC/MS/MS, which detects LFLX oxidation byproducts (Hubicka et al, 2013), RP-HPLC, HPLC, microbiological methods for LFLX detection in biological fluids (Chang, Wang, & Tsai, 2010;El-Shanawany, El-Adl, Abdel-Aziz, Hashem, & Sebaiy, 2011;Wu et al, 2014), spectrophotometric methods for aqueous LFLX samples, and Smart polymer aqueous two-phase flotation system for LFLX detection in food samples (Jain, 2012;Lu et al, 2016). Most of these methods are expensive, time-consuming, and require skilled personnel.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Mukunzi

Isanga

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

View full text Add to dashboard Cite

The misuse and illegal use of fluoroquinolones (FQs) in animal-based food products have drawn considerable attention in several countries. As a result, there has been an increased demand for efficient detection methods of FQs in food products. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic strip device based on a monoclonal antibody against lomefloxacin (LFLX), a second generation FQ. Ic-ELISA had an IC 50 value of 0.19 ng/mL with a limit of detection of 0.04 ng/mL in 0.01 M phosphate-buffered saline (PBS). The intra-and inter-assay recovery rates of LFLX in bovine milk samples were 98.02-107.40% and 100.65-107.82%. The immunochromatographic assay of LFLX in PBS and spiked bovine milk samples had visual cutoff values at 1 and 5.0 ng/mL, respectively, with significant cross-reactivity with norfloxacin and enoxacin. The developed ic-ELISA and strip method may assist in the detection of FQs in foods.ARTICLE HISTORY

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Mukunzi

Isanga

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…Synthesis of cell wall inhibit by the bactericidal action of cephalosporin, which inhibits the final transpeptidation step of the peptidoglycan synthesis in the bacterial cell wall, thus inhibiting biosynthesis of cell wall assembly by arresting, resulting in bacterial cell death. Elbashir et al, 2012, Khan et al, 2011, Nemutlu et al, 2009, Pulgarín et al, 2013, Hubicka et al, 2013, Lalitha Devi et al, 2009, Patel et al, 2011, Shervington et al, 2005and Deekonda et al, 2014 reported various analytical methods, like RP-HPLC/UV detection method, Spectrofluorimetric method, Capillary Electrophoresis, UPLC-MS/MS. Validated stability-indicating RP-HPLC methods have been already reported.…”

Section: Introductionmentioning

confidence: 99%

Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Ofloxacin and Cefixime in their Combined Dosage Form

Jain¹,

Jain²,

Jain³

2015

Sci. Technol. Arts Res. J.

View full text Add to dashboard Cite

Article Information The objective of the current study was to develop and validate a simple, accurate, precise and selective stability-indicating gradient reverse phase high performance liquid chromatographic method for simultaneous estimation of Ofloxacin and Cefixime in pharmaceutical formulation in presence of degradation products. The chromatographic separation of Ofloxacin and Cefixime was achieved on Shimadzu LC-20AT series HPLC having C18-ODS bonded column (250 ×4.6 mm, 40 °C, 10 μL) using UV/Visible detector at 276 nm. The optimized mobile phase was consisted of a methanol: phosphate buffer (50:50) at a flow rate of 1.0 ml/m. The retention times were 4.799 and 1.602 m for Ofloxacin and Cefixime respectively. The proposed method provided linear responses within the concentration ranges 5-25 µg/ml for Ofloxacin and Cefixime both. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 0.0259, 0.078 µg/ml and 0.0206, 0.062 µg/ml for Ofloxacin and Cefixime F respectively. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision, robustness and ruggedness. The studies data revealed that developed method was convenient, fairly reliable, sensitive, less expensive and reproducible.

show abstract

“…Recently, the methods for norfloxacin residue analysis in foodstuff and environment are generally based on chromatographic techniques (Hubicka et al 2013;Ulu 2012;Sher et al 2010;Hancu et al 2012;Liu et al 2010;Solangi et al 2009;Yang et al 2008;Alnajjar et al 2007), spectrophotometry (Yi et al 2011;Basak et al 2010), electrochemiluminescence (Kamruzzaman et al 2011;Sun et al 2009), and immunoassay (IA). Currently, IA has been proven to be a sensitive and reliable method, in which, except for the traditional immunoanalytical methods (Cui et al 2011;Sheng et al 2011;Liu et al 2013;Fan et al 2012), surface plasmon resonance biosensor immunoassay (Huet et al 2009;Marchesini et al 2007) and immunoaffinity chromatographic (Zhao et al 2009) methods are also available for the determination of norfloxacin in food samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

et al. 2014

View full text Add to dashboard Cite

A simple and sensitive capillary electrophoresis immunoassay method with laser-induced fluorescence detector was developed for the determination of norfloxacin in food samples. The assay was based on the competitive immunoreaction between fluorescein isothiocyanate-labeled norfloxacin tracer and free norfloxacin with a limited amount of antinorfloxacin antibody. In this study, the experimental parameters affecting the norfloxacin determination were systematically optimized including incubation time, buffer pH and concentration, and separation voltage. Under the optimal conditions, norfloxacin could be accurately quantified within 3 min using Na 2 B 4 O 7 /NaH 2 PO 4 buffer (30 mmol/L, pH 8.0) for background electrolyte and 25 kV for the separation voltage. The linear range of the method was 0.08-50 μg/L with a detection limit of 0.005 μg/L. The relative standard deviations for migration time and peak area of the immunocomplex were 0.17 % (intraday) and 3.46 % (intraday), respectively. The proposed method has been further applied to the determination of norfloxacin in milk, chicken, pork, and fish samples, exhibiting satisfactory recovery and sensitivity. These results indicated that the proposed method might serve as an efficient tool for the selective determination of norfloxacin in food samples.

show abstract

Separation and characterization of ciprofloxacin, difloxacin, lomefloxacin, norfloxacin, and ofloxacin oxidation products under potassium permanganate treatment in acidic medium by UPLC-MS/MS

Cited by 44 publications

References 17 publications

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Ofloxacin and Cefixime in their Combined Dosage Form

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

Contact Info

Product

Resources

About