Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Ofloxacin and Cefixime in their Combined Dosage Form

Abstract

Article Information The objective of the current study was to develop and validate a simple, accurate, precise and selective stability-indicating gradient reverse phase high performance liquid chromatographic method for simultaneous estimation of Ofloxacin and Cefixime in pharmaceutical formulation in presence of degradation products. The chromatographic separation of Ofloxacin and Cefixime was achieved on Shimadzu LC-20AT series HPLC having C18-ODS bonded column (250 ×4.6 mm, 40 °C, 10 μL) using UV/Visible detector at 276 nm. The optimized mobile phase was consisted of a methanol: phosphate buffer (50:50) at a flow rate of 1.0 ml/m. The retention times were 4.799 and 1.602 m for Ofloxacin and Cefixime respectively. The proposed method provided linear responses within the concentration ranges 5-25 µg/ml for Ofloxacin and Cefixime both. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 0.0259, 0.078 µg/ml and 0.0206, 0.062 µg/ml for Ofloxacin and Cefixime F respectively. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision, robustness and ruggedness. The studies data revealed that developed method was convenient, fairly reliable, sensitive, less expensive and reproducible.

Functionality Advancement of Poorly Soluble Drug by Comparative Study of Solubilizing Techniques with Molecular Simulation to in vivo evaluation

“…The sorting was performed using a Cosmosil C18 packed column (250 mm × 4.6 mm,5 μm) at 276 nm. The mobile phase consisted of a mixing of phosphate buffer having pH 4±0.05 with methyl alcohol(50:50) operating at a flow rate of 1 ml/min 36 .…”

Kolestrolü Tedavi Edici İlaç Kombinasyonlarının Eşzamanlı Spektrofotometrik Tayini

“…Literatürde atorvasatin ve ezetimib etken maddenin farklı analitik yöntemlerle analizleri mevcuttur. Ezetimib etken maddesinin atorvasatin etken maddesinden farklı ilaç grupları ile ters faz yüksek basınçlı sıvı kromatografi yöntemi ile [4,5,6,7], sıvı kromatogarfi-kütle/kütle spektrometri ile (LC-MS/MS) [8], voltametrik yöntemle [9], spektrofotometrik yöntemle [10]; atorvastatin etken maddesinin yüksek performans ince tabaka metodu ile [11], spektrofotometrik metotla [12,13] analizleri yapılmıştır.…”