Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Salvo, Pietro; Dini, Valentina; Kirchhain, Arno; Janowska, Agata; Oranges, Teresa; Chiricozzi, Andrea; Lomonaco, T; Francesco, Fabio Di; Romanelli, Marco

doi:10.3390/s17122952

Cited by 94 publications

(49 citation statements)

References 132 publications

(156 reference statements)

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“…Briefly, equally sized and healthy A. cepa bulbs were immersed in tap water and allowed to develop meristematic roots at room temperature for 72 h. Once roots were at least 2 cm long, bulbs were transferred into glass beakers containing the test solutions: 30 Figure 1a). Glutaraldehyde efficiently traps the IAd thanks to the terminal -NH2 group [61].…”

Section: Genotoxicitymentioning

confidence: 99%

A Voltammetric pH Sensor for Food and Biological Matrices

Vivaldi¹,

Santalucia²,

Poma³

et al. 2020

Preprint

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<div>Measurement of pH is of fundamental importance in a wide range of environmental, biological and industrial applications. Glass electrode and litmus paper are widely used for this, but the former is difficult to miniaturize, prone to drift and fragile, the latter is inaccurate. This paper describes a pH sensor based on an indoaniline-derivative (4-((4-aminophenyl)imino)-2,6-dimethoxycyclohexa-2,5dien-1-one), which exploits alternating current voltammetry to measure pH in the range between 2 and 12 . The synthetized indoaniline-derivative was not genotoxic (A. cepa assay), and the sensor reliably measured pH in milk, tea, orange juice, blood, urine and saliva. Results were comparable with those obtained with a glass electrode calibrated with certified solutions (maximum relative standard deviation of 3 % and accuracy less than 0.2 pH unit). The sensor had negligible hysteresis, an almost Nernstian sensitivity (56 mV/pH) and was fully functional after a two-month storage. Sensor response showed a limited dependence on temperature (0.14 mV per pH unit and °C) and limited sensitivity to possible interferents such as lithium and sodium ions; its response to these was similar to that of a glass electrode, and was absent for ascorbic acid. </div>

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Section: Genotoxicitymentioning

confidence: 99%

A Voltammetric pH Sensor for Food and Biological Matrices

Vivaldi¹,

Santalucia²,

Poma³

et al. 2020

Preprint

Self Cite

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“…In blood sera of healthy individuals, CRP is known to be present usually in a small amount. CRP production in the circulation elevates its level in serum in response to various inflammations, infection, stress, trauma, surgery, tissue damage, cardiovascular disease, and neurological degeneration [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. During an acute phase response, the CRP level in serum increases rapidly (by more than 100-fold) and reaches a peak within 48 h with its half-life of about 19 h [ 9 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Amid the methodologies mentioned above, optical plasmonic sensors have attracted much attention for detecting biomarker molecules without labels such as fluorescent tags, due to a number of advantages [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. They include the high sensitivity without contaminating target biomolecules, the capability of multiplexed assay, a small amount of an analyte sample required, the relative immunity to external factors (ambient temperature variation/external electric disturbance), and the observability of time-dependent kinetic behavior of bio-molecular interactions [ 3 , 60 ].…”

Section: Introductionmentioning

confidence: 99%

Plasmonic Optical Biosensors for Detecting C-Reactive Protein: A Review

Seok

2020

Micromachines

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C-reactive protein (CRP), a potent acute-phase reactant that increases rapidly in response to inflammation, tissue damage or infections, is also considered an indicator of the risk of cardiovascular diseases and neurological disorders. Recent advances in nanofabrication and nanophotonic technologies have prompted the optical plasmonic phenomena to be tailored for specific detection of human serum CRP into label-free devices. We review the CRP-specific detection platforms with high sensitivity, which feature the thin metal films for surface plasmon resonance, nano-enhancers of zero dimensional nanostructures, and metal nanoparticles for localized surface plasmon resonance. The protocols used for various types of assay reported in literature are also outlines with surface chemical pretreatment required for specific detection of CRPs on a plasmonic surface. Properties including sensitivity and detection range are described for each sensor device reviewed, while challenges faced by plasmonic CRP sensors are discussed in the conclusion, with future directions towards which research efforts need to be made.

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“…Protein biomarkers like CRP and IL6 are individually analyzed following conventional techniques such as immunoassays or flow cytometry, which are precise and reliable, but they are also time consuming and require specialized personnel [11]. For the detection of circulating miRNAs, the most common techniques are real-time reversetranscription polymerase chain reaction (qRT-PCR), northern blot and fluorescent microarray technology.…”

Section: Introductionmentioning

confidence: 99%

Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor

Fabri-Faja

Calvo-Lozano

Dey

et al. 2019

Analytica Chimica Acta

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Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient´s evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical pointof-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6) and miRNAs, using miRNA-16 as example. Limits of Detection (LoD) of 18 µg mL-1 , 88 µg mL-1 and 1 µM (6 µg mL-1) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers.

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Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Cited by 94 publications

References 132 publications

A Voltammetric pH Sensor for Food and Biological Matrices

A Voltammetric pH Sensor for Food and Biological Matrices

Plasmonic Optical Biosensors for Detecting C-Reactive Protein: A Review

Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor

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