Plasmonic Optical Biosensors for Detecting C-Reactive Protein: A Review

Seok, Joo Seon; Ju, Heongkyu

doi:10.3390/mi11100895

Cited by 21 publications

(17 citation statements)

References 101 publications

(160 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many works have a narrower scope and overview the variety of analytical methods and biosensing technologies for detecting specific biomarkers (proteins, peptides, enzymes) or pathogens: cancer biomarkers [ 135 ] (2021), [ 136 ] (2021), [ 137 ] (2021), [ 138 ] (2018), rheumatoid arthritis (Ra) biomarkers [ 139 ] (2020), Alzheimer’s biomarkers (tau-protein) [ 140 ] (2020), [ 141 ] (2020), inflammation markers, (C-reactive protein) [ 142 ] (2020), [ 143 ] (2020), [ 144 ] (2018).…”

Section: Label-free Optical Techniques Of Protein Detection Quantific...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

View full text Add to dashboard Cite

Proteins in biological fluids (blood, urine, cerebrospinal fluid) are important biomarkers of various pathological conditions. Protein biomarkers detection and quantification have been proven to be an indispensable diagnostic tool in clinical practice. There is a growing tendency towards using portable diagnostic biosensor devices for point-of-care (POC) analysis based on microfluidic technology as an alternative to conventional laboratory protein assays. In contrast to universally accepted analytical methods involving protein labeling, label-free approaches often allow the development of biosensors with minimal requirements for sample preparation by omitting expensive labelling reagents. The aim of the present work is to review the variety of physical label-free techniques of protein detection and characterization which are suitable for application in micro-fluidic structures and analyze the technological and material aspects of label-free biosensors that implement these methods. The most widely used optical and impedance spectroscopy techniques: absorption, fluorescence, surface plasmon resonance, Raman scattering, and interferometry, as well as new trends in photonics are reviewed. The challenges of materials selection, surfaces tailoring in microfluidic structures, and enhancement of the sensitivity and miniaturization of biosensor systems are discussed. The review provides an overview for current advances and future trends in microfluidics integrated technologies for label-free protein biomarkers detection and discusses existing challenges and a way towards novel solutions.

show abstract

Section: Label-free Optical Techniques Of Protein Detection Quantific...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Another way to reduce the effect of non-specific binding and the LOD is by increasing the signal-to-noise ratio via adding labels that selectively bind to the target molecules, thus increasing the optical signal associated with the target capture event. Just like the surface receptors for the molecular target capture, these labels need to be custom-engineered to provide target selectivity and added to the assay together with the sample [86][87][88][89][90][91]. This further complicates the process and increases the initial development time of the sensor technology.…”

Section: Affinity-type Biosensors With Optical Signal Amplificationmentioning

confidence: 99%

Roadmap on Universal Photonic Biosensors for Real-Time Detection of Emerging Pathogens

et al. 2021

View full text Add to dashboard Cite

The COVID-19 pandemic has made it abundantly clear that the state-of-the-art biosensors may not be adequate for providing a tool for rapid mass testing and population screening in response to newly emerging pathogens. The main limitations of the conventional techniques are their dependency on virus-specific receptors and reagents that need to be custom-developed for each recently-emerged pathogen, the time required for this development as well as for sample preparation and detection, the need for biological amplification, which can increase false positive outcomes, and the cost and size of the necessary equipment. Thus, new platform technologies that can be readily modified as soon as new pathogens are detected, sequenced, and characterized are needed to enable rapid deployment and mass distribution of biosensors. This need can be addressed by the development of adaptive, multiplexed, and affordable sensing technologies that can avoid the conventional biological amplification step, make use of the optical and/or electrical signal amplification, and shorten both the preliminary development and the point-of-care testing time frames. We provide a comparative review of the existing and emergent photonic biosensing techniques by matching them to the above criteria and capabilities of preventing the spread of the next global pandemic.

show abstract

“…Their application in the biosensing field is further warranted given the versatile incorporation in their constitutional framework of metallic entities. Gold nanoparticles (AuNPs) are stable and biocompatible metallic entities with unique electrical, optical and surface chemical properties AuNPs characterized by strong adsorption ability, large specific surface area and formation of surface plasmon resonance band [6] were applied in various sensing formats (i.e., direct, sandwich or NP enhanced) involving bio-recognition mechanisms [7], catalysis and biosynthesis [8]. Their surface functionalization sheds light on a diverse collection of functional moieties that can be used for biomolecular interaction.…”

Section: Introduction 1dynamers and Their Analytical Potentialmentioning

confidence: 99%

Real Time SPR Assessment of the Structural Changes of Adaptive Dynamic Constitutional Frameworks as a New Route for Sensing

et al. 2022

View full text Add to dashboard Cite

Cross linked gold-dynamic constitutional frameworks (DCFs) are functional materials of potential relevance for biosensing applications, given their adaptivity and high responsivity against various external stimuli (such as pH, temperature) or specific interactions with biomolecules (enzymes or DNA) via internal constitutional dynamics. However, characterization and assessment of their dynamic conformational changes in response to external stimuli has never been reported. This study proves the capability of Surface Plasmon Resonance (SPR) assays to analyse the adaptive structural modulation of a functional matrix encompassing 3D gold-dynamic constitutional frameworks (Au-DCFs) when exposed to pH variations, as external stimuli. We analyse Au-DCFs formed from Au nanoparticles, (AuNP) connected through constitutionally dynamic polymers, dynamers, with multiple functionalities. For increased generality of this proof-of-concept assay, Au-DCFs, involving DCFs designed from 1,3,5-benzene-tricarbaldehyde (BTA) connecting centres and polyethylene glycol (PEG) connectors, are covalently attached to standard SPR sensing chips (Au nanolayers, carboxyl terminated or with carboxymethyl dextran, CMD top-layer) and analysed using state-of-the art SPR instrumentation. The SPR effects of the distance from the Au-DCFs matrix to the Au nanolayer of the sensing chip, as well as of Au-DCFs thickness were investigated. This study reveals the SPR response, augmented by the AuNP, to the conformational change, i.e., shrinkage, of the dynamer and AuNP matrix when decreasing the pH, and provides an unexplored insight into the sensing applicability of SPR real-time analysis of adaptive functional materials.

show abstract

Plasmonic Optical Biosensors for Detecting C-Reactive Protein: A Review

Cited by 21 publications

References 101 publications

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Roadmap on Universal Photonic Biosensors for Real-Time Detection of Emerging Pathogens

Real Time SPR Assessment of the Structural Changes of Adaptive Dynamic Constitutional Frameworks as a New Route for Sensing

Contact Info

Product

Resources

About