Nanotheranostics, which combines optical multiplexed disease detection with therapeutic monitoring in a single modality, has the potential to propel the field of nanomedicine toward genuine personalized medicine. Currently employed mainstream modalities using gold nanoparticles (AuNPs) in diagnosis and treatment are limited by a lack of specificity and potential issues associated with systemic toxicity. Light‐mediated nanotheranostics offers a relatively non‐invasive alternative for cancer diagnosis and treatment by using AuNPs of specific shapes and sizes that absorb near infrared (NIR) light, inducing plasmon resonance for enhanced tumor detection and generating localized heat for tumor ablation. Over the last decade, significant progress has been made in the field of nanotheranostics, however the main biological and translational barriers to nanotheranostics leading to a new paradigm in anti‐cancer nanomedicine stem from the molecular complexities of cancer and an incomplete mechanistic understanding of utilization of Au‐NPs in living systems. This work provides a comprehensive overview on the biological, physical and translational barriers facing the development of nanotheranostics. It will also summarise the recent advances in engineering specific AuNPs, their unique characteristics and, importantly, tunability to achieve the desired optical/photothermal properties.
The morphology of plasmonic nano-assemblies has a direct influence on optical properties, such as localised surface plasmon resonance (LSPR) and surface enhanced Raman scattering (SERS) intensity.
In the past few years, remarkable progress has been made in unveiling novel and unique optical properties of strongly coupled plasmonic nanostructures. However, the application of such plasmonic nanostructures in biomedicine remains challenging because of the lack of facile and robust assembly methods for producing stable nanostructures. Previous attempts to achieve plasmonic nanoassemblies using molecular ligands were limited by the lack of flexibility that could be exercised in forming them. Here, we report the utilization of tailor-made hyperbranched polymers (HBP) as linkers to assemble gold nanoparticles (NPs) into nanoassemblies. The ease and flexibility in tuning the particle size and number of branch ends of an HBP make it an ideal candidate as a linker, as opposed to DNA, small organic molecules, and linear or dendrimeric polymers. We report a strong correlation of polymer (HBP) concentration with the size of the hybrid nanoassemblies and "hot-spot" density. We have shown that such solutions of stable HBP-gold nanoassemblies can be barcoded with various Raman tags to provide improved surface-enhanced Raman scattering (SERS) compared to that of nonaggregated NP systems. These Raman-barcoded hybrid nanoassemblies, with further optimization of the NP shape, size, and hot-spot density, may find application as diagnostic tools in nanomedicine.
Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient´s evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical pointof-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6) and miRNAs, using miRNA-16 as example. Limits of Detection (LoD) of 18 µg mL-1 , 88 µg mL-1 and 1 µM (6 µg mL-1) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers.
Plasmonic biosensors can be conveniently used as portable diagnostic devices for attaining timely and cost-effective clinical outcomes. Nanoplasmonics technology opens the way for sensor miniaturization, multiplexing and point of care testing.
We propose an approach for the prediction of the depth of a single buried object within a turbid medium combining spatially offset Raman spectroscopy (SORS) and transmission Raman spectroscopy (TRS) and relying on differential attenuation of individual Raman bands brought about by the spectral variation of matrix absorption (and scattering). The relative degree of the Raman band changes is directly related to the path length of Raman photons traveling through the medium, thereby encoding the information on the depth of the object within the matrix. Through a calibration procedure with root mean square error of calibration (RMSEC) = 3.4%, it was possible to predict the depth of a paracetamol (acetaminophen) inclusion within a turbid matrix consisting of polyethylene (PE) by monitoring the relative intensity of two Raman bands of paracetamol exhibiting differential absorption by the matrix. The approach was shown to be largely insensitive to variations of the amount of the inclusion (paracetamol) and to the overall thickness of the turbid matrix (PE) with a root mean square error of prediction (RMSEP) maintained below 10% for the tested cases. This represents a major advantage over previously demonstrated comparable depth determination Raman approaches (with the exception of full Raman tomography requiring complex mathematical reconstruction algorithms). The obtained experimental data validate the proposed approach as an effective tool for the noninvasive determination of the depth of buried objects in turbid media with potential applications including determining noninvasively the depth of a lesion in cancer diagnosis in vivo.
Spatially offset Raman spectroscopy (SORS) is a technique for interrogating the subsurface composition of turbid samples noninvasively. This study generically addresses a fundamental question relevant to a wide range of SORS studies, which is how deep SORS probes for any specific spatial offset when analyzing a turbid sample or, in turn, what magnitude of spatial offset one should select to probe a specific depth. This issue is addressed by using Monte Carlo simulations, under the assumption of negligible absorption, which establishes that the key parameter governing the extent of the probed zone for a point-like illumination and point-like collection SORS geometry is the reduced scattering coefficient of the medium. This can either be deduced from literature data or directly estimated from a SORS measurement by evaluating the Raman intensity profile from multiple spatial offsets. Once this is known, the extent of the probed zone can be determined for any specific SORS spatial offset using the Monte Carlo simulation results presented here. The proposed method was tested using experimental data on stratified samples by analyzing the signal detected from a thin layer that was moved through a stack of layers using both non-absorbing and absorbing samples. The proposed simple methodology provides important additional information on SORS measurements with direct relevance to a wide range of SORS applications including biomedical, pharmaceutical, security, forensics, and cultural heritage.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.