Sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to Leishmania (Viannia) guyanensis

Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre

“…PCR was the most sensitive and specific exam among the applied methods. These findings were in agreement with other Brazilian studies using the same or different primers (Pirmez et al 1999, Romero et al 2001, Oliveira et al 2005, Marques et al 2006.…”

Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

“…The current state of the art diagnosis of L. siamensis infection relies on PCR and nucleotide sequencing. These techniques are more sensitive than others, 6,9,[28][29][30][31] and they can now be performed in most provincial and university hospitals in Thailand. Although use of traditional screening tests for this disease is being investigated, PCR could be used for survey and surveillance studies, including asymptomatic persons.…”

Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil

“…These primers have been extensively used in Brazil for genus-specific diagnosis of Leishmania infection (Degrave et al 1994a, b). Authors have described those primers with different names and notations (Pirmez et al 1999, Romero et al 2001a, Volpini et al 2004, De Assis et al 2008, Luz et al 2009). The amplification procedure was performed in a Perkin Elmer GeneAmp PCR System 2400 thermocycler (The Perkin Elmer Corporation, Norwalk, CT, USA).…”