Sensitive lateral flow immunoassay of an antibiotic neomycin in foodstuffs

Hendrickson, Olga D.; Byzova, Nadezhda A.; Zvereva, Elena A.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1007/s13197-020-04541-z

Cited by 24 publications

(12 citation statements)

References 29 publications

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“…The lateral flow tests are a simple analytical tool that has spread since the 1980s. The first applications of lateral flow tests were focused on revealing but further analytes followed, and the tests gained popularity as tools for the analysis of biochemical and immunochemical markers, toxins, microorganisms, pollutants, drugs, pharmaceutics and others [ 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. These devices resemble biosensors and some scholars consider them a type of biosensor.…”

Section: Current Point-of-care Tests For Covid-19mentioning

confidence: 99%

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Pohanka

2022

Sensors

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Coronavirus disease 2019 (COVID-19) is a highly virulent infection that has caused a pandemic since 2019. Early diagnosis of the disease has been recognized as one of the important approaches to minimize the pathological impact and spread of infection. Point-of-care tests proved to be substantial analytical tools, and especially lateral flow immunoassays (lateral flow tests) serve the purpose. In the last few years, biosensors have gained popularity. These are simple but highly sensitive and accurate analytical devices composed from a selective molecule such as an antibody or antigen and a sensor platform. Biosensors would be an advanced alternative to current point-of-care tests for COVID-19 diagnosis and standard laboratory methods as well. Recent discoveries related to point-of-care diagnostic tests for COVID-19, the development of biosensors for specific antibodies and specific virus parts or their genetic information are reviewed.

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Section: Current Point-of-care Tests For Covid-19mentioning

confidence: 99%

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Pohanka

2022

Sensors

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“…The troponin test, owing to the specificity of mAbs against TnI clones IS7 and 6F9 (HyTest, Finland), differentiated the types of raw meat materials since mAbs only detected troponin in mammalian meat, not in birds. The same group of authors developed test systems to detect antibiotics in foods: aminoglycoside neomycin used in agriculture and veterinary medicine and presumably contaminated foodstuffs [71], and for the simultaneous determination of tylosin and lincomycin-antibiotics of the classes of macrolides and lincosamides that are also widely used in animal husbandry and implicated in food contamination [72,73].…”

Section: Immunochemical Detection Systems For Assaying Foodstuffsmentioning

confidence: 99%

“…Figure 4. Useful modifications of nanoparticles for improvement of the analyte detection described in this review: AuNPs[65][66][67][68][69][70][71][72][73], Superspherical AuNPs[74], Pt-nanoflower-type NPs[75], Au@PTNps[76], Au@Ag-PtNPs[77], quantum dots[78][79][80], magnetic beads[81].…”

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confidence: 99%

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.

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“…In comparison with instrumental methods, immunoassays are simple and cheap, so they are usually used for the rapid screening of a large number of samples. By now, there have been different types of immunoassays reported to detect AGs, including enzyme-linked, immunosorbent assays [ 5 , 6 , 7 , 8 , 9 , 10 , 11 ], fluorometric immunoassays [ 12 ], chemiluminescent immunoassays [ 13 ], immunochromatographic strips [ 14 , 15 , 16 , 17 ], and biosensors [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, the recognition abilities of these reagents are limited. For example, most of the antibodies [ 6 , 8 , 9 , 12 , 13 , 14 , 15 , 16 , 17 ] and all the aptamers [ 18 ] can only recognize one drug, and the antibodies showing the broadest recognition spectrum can recognize at most two Ags [ 5 , 10 , 11 ]. This means that an immunoassay capable of multi-determining Ags residues has not been reported so far, which is because a broad specific recognition reagent for Ags is not available.…”

Section: Introductionmentioning

confidence: 99%

Development of a Fluorescence Polarization Assay for Multi-Determination of 10 Aminoglycosides in Pork Muscle Sample Based on Ribosomal Protein S12 and Studying Its Recognition Mechanism

Xia

Zhang

Wang

2022

Foods

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The residues of aminoglycosides in foods of animal origin are a potential risk to consumers. There have been some immunoassays reported for the screening of aminoglycoside residues, but the method showing the broadest detection spectrum can only be used to detect two drugs. This is because a broad specific recognition reagent is not available. In the present study, the receptor of aminoglycosides (ribosomal protein S12 of Lysinibacillus sphaericus) was expressed, and its affinities and recognition mechanisms for 10 aminoglycosides were studied by using surface plasmon resonance and molecular docking, respectively. Then the receptor was used as a recognition reagent to develop a fluorescence polarization assay on a 96-well microplate for the detection of the 10 drugs in pork muscle samples. The limits of detection for the 10 drugs ranged from 5.25 to 30.25 ng/g. The sensitivities for the 10 drugs were generally consistent with their respective receptor affinities and binding energies. After comprehensive comparison, the method performances were better than all the previously reported immunoassays for aminoglycosides. This is the first study reporting the recognition mechanisms of ribosomal protein S12 of Lysinibacillus sphaericus for 10 aminoglycosides and the use of it as a recognition reagent to develop a pseudo-immunoassay for the multi-determination of aminoglycosides in food samples.

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Sensitive lateral flow immunoassay of an antibiotic neomycin in foodstuffs

Cited by 24 publications

References 29 publications

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Development of a Fluorescence Polarization Assay for Multi-Determination of 10 Aminoglycosides in Pork Muscle Sample Based on Ribosomal Protein S12 and Studying Its Recognition Mechanism

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