Sensitive immunochemical approaches for quantitative (FPIA) and qualitative (lateral flow tests) determination of gentamicin in milk

Beloglazova, Natalia V.; Shmelin, P. S.; Еремин, С. А.

doi:10.1016/j.talanta.2015.11.060

Cited by 37 publications

(11 citation statements)

References 27 publications

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“…Gent-FITC and Kana-FITC were synthesized as previous reported (Beloglazova, Shmelin, & Eremin, 2016). Firstly, the 10 µmol Gent/Kana and the equal FITC were dissolved in 1 mL methanol containing 50 µL TEA.…”

Section: Synthesis Of Gent-fitc and Kana-fitcmentioning

confidence: 99%

Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA

et al. 2017

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Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC of FPIA and ic-ELISA based anti-Kana IgY were 7.97±0.9μg/mL and 0.15±0.01μg/mL. The limits of detection (LOD, IC) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007μg/mL, respectively. The LOD for ic-ELISA were both 0.001μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.

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Section: Synthesis Of Gent-fitc and Kana-fitcmentioning

confidence: 99%

Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA

et al. 2017

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“…Another sensitive quantitative FPIA immunochemical approach has been reported for the screening of gentamicin in milk samples. The observed limit of detection was 5 μg kg −1 (Beloglazova, Shmelin, & Eremin, 2016). Shanin, Shaimardanov, Thai, and Eremin (2015) developed one reagent FPIA for the screening of levofloxacin in urine samples (Shanin et al, 2015).…”

Section: Fluorescence Immunoassay/fluorescence Polarization Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

116

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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“…Quantum dots (QD), which have high fluorescence intensity, strong anti-photobleaching ability, and superior performance compared with traditional dyes, have caused widespread concern. The use of QD as sensors in immunochromatography assays is an active area of research (Berlina et al, 2013;Beloglazova et al, 2016). For instance, Morales-Narváez et al (2015) established a turn-on detection mode of lateral flow strip by combining QD and graphene oxide.…”

Section: Luminescent Nanoparticlesmentioning

confidence: 99%

Invited review: Advancements in lateral flow immunoassays for screening hazardous substances in milk and milk powder

Chen

Huang

Song

et al. 2019

Journal of Dairy Science

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Dairy-related food safety outbreaks, such as foodborne pathogen contamination, mycotoxin contamination, and veterinary drug contamination, sometimes happen and have been reported all over the world, affecting human health and, in some cases, leading to death. Thus, rapid yet robust detection methods are needed to monitor milk and milk powder for the presence of hazardous substances. The lateral flow immunoassay (LFI) is widely used in onsite testing because of its rapidity, simplicity, and convenience. In this review, we describe some traditional LFI used to detect hazardous substances in milk and milk powder. Furthermore, we discuss recent advances in LFI that aim to improve sensitivity or detection efficiency. These advances include the use of novel label materials, development of signal amplification systems, design of multiplex detection systems, and the use of nucleic acid-based LFI.

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Sensitive immunochemical approaches for quantitative (FPIA) and qualitative (lateral flow tests) determination of gentamicin in milk

Cited by 37 publications

References 27 publications

Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA

Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA

Current advances in immunoassays for the detection of antibiotics residues: a review

Invited review: Advancements in lateral flow immunoassays for screening hazardous substances in milk and milk powder

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