Sensitive Detection of Single-Nucleotide Polymorphisms by Solid Nanopores Integrated With DNA Probed Nanoparticles

Abstract

Single-nucleotide polymorphisms (SNPs) are the abundant forms of genetic variations, which are closely associated with serious genetic and inherited diseases, even cancers. Here, a novel SNP detection assay has been developed for single-nucleotide discrimination by nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The SNP of p53 gene mutation in gastric cancer has been successfully detected in the femtomolar concentration by nanopore sensing. The robust biosensing strategy offers a way for solid nanopore sensors integrated with varied nanoparticles to achieve single-nucleotide distinction with high sensitivity and spatial resolution, which promises tremendous potential applications of nanopore sensing for early diagnosis and disease prevention in the near future.

Recent progress of biosensors for the detection of lung cancer markers

“…An SNP detection method for single nucleotide recognition based on a nanopore sensing platform was established by using gold nanoparticles of DNA probes as carriers based on nanopore sensing technology, and SNPs mutated in the p53 gene in gastric cancer tissues were successfully detected in the nanomolar concentration range. 284 The ionic current signal of the oxford nanopore technologies MinION maps methylation to cytosine and adenosine and sorts individual C, 5-MC, and 5-HMC bases based on single molecules of synthetic oligonucleotides, enabling the detection of the genomic DNA variation of the methylation level with E. coli growth stages. 285 Protein markers.…”

Large-scale production of polyimide micropore-based flow cells for detecting nano-sized particles in fluids

“…However, polyethylene terephthalate (PET) conical pores have uctuated the current that can reach 100% of the signal when a constant voltage is applied. [19][20][21][22] PI nanopores display very stable electrical currents over different electrolytes and pHs. PI was used to create micropores because of its higher mechanical properties, greater thermal stability, and superior insulating properties versus other polymers.…”

“…As faster translocations imply greater surface charges of the nanoparticle, the zeta potential of the particle is measured as the slope of translocation time with voltage. 6,[15][16][17] Nanoparticles are particularly useful for the detection of biomolecules such as DNA, [18][19][20][21] proteins, [22][23][24][25] and viruses 26,27 by solid-state nanopores. Functionalized nanoparticles with target-specic receptors will change in size when bound to targets.…”