Large-scale production of polyimide micropore-based flow cells for detecting nano-sized particles in fluids

Salehirozveh, Mostafa; Porro, Alessandro; Thei, Federico

doi:10.1039/d2ra07423k

Cited by 1 publication

(1 citation statement)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, specialized equipment and associated support from skilled technicians are necessary for the detection of UZn by atomic absorption spectroscopy, inductively coupled plasma mass spectrometry (ICP-MS), and surface-enhanced Raman spectroscopy (SERS). ,− Colorimetric analysis using reagents such as o-2-(2-hydroxy-5-sulfophenylazo) benzylidenehydrazinobenzoic acid was assessed as a convenient alternative means to detect UZn in patient samples . However, such convenient alternative methodologies tend to suffer from low specificity because of their inability to differentiate metal ions like Zn 2+ and Cu 2+ present in urine .…”

Section: Introductionmentioning

confidence: 99%

Rapid Near-Patient Impedimetric Sensing Platform for Prostate Cancer Diagnosis

Dehghani,

Karthikeyan,

Tajabadi

et al. 2024

ACS Omega

View full text Add to dashboard Cite

With the global escalation of concerns surrounding prostate cancer (PCa) diagnosis, reliance on the serologic prostatespecific antigen (PSA) test remains the primary approach. However, the imperative for early PCa diagnosis necessitates more effective, accurate, and rapid diagnostic point-of-care (POC) devices to enhance the result reliability and minimize diseaserelated complications. Among POC approaches, electrochemical biosensors, known for their amenability and miniaturization capabilities, have emerged as promising candidates. In this study, we developed an impedimetric sensing platform to detect urinary zinc (UZn) in both artificial and clinical urine samples. Our approach lies in integrating label-free impedimetric sensing and the introduction of porosity through surface modification techniques. Leveraging a cellulose acetate/reduced graphene oxide composite, our sensor's recognition layer is engineered to exhibit enhanced porosity, critical for improving the sensitivity, capture, and interaction with UZn. The sensitivity is further amplified by incorporating zincon as an external dopant, establishing highly effective recognition sites. Our sensor demonstrates a limit of detection of 7.33 ng/mL in the 0.1−1000 ng/mL dynamic range, which aligns with the reference benchmark samples from clinical biochemistry. Our sensor results are comparable with the results of inductively coupled plasma mass spectrometry (ICP-MS) where a notable correlation of 0.991 is achieved. To validate our sensor in a real-life scenario, tests were performed on human urine samples from patients being investigated for prostate cancer. Testing clinical urine samples using our sensing platform and ICP-MS produced highly comparable results. A linear correlation with R 2 = 0.964 with no significant difference between two groups (p-value = 0.936) was found, thus confirming the reliability of our sensing platform.

show abstract