Sensitive and specific detection of potentially allergenic almond (Prunus dulcis) in complex food matrices by Taqman® real-time polymerase chain reaction in comparison to commercially available protein-based enzyme-linked immunosorbent assay

A Cherry Seed‐Derived Spice, Mahleb, is Recognized by Anti‐Almond Antibodies Including Almond‐Allergic Patient IgE

“…Western blots clearly demonstrated that many of the almond kernel proteins share epitopes with their cherry kernel protein homologues (Figure C). In line with these observations are those of a previous report demonstrated significant cross‐reactivity between almond and kernels of other members of the Prunoideae subfamily, including peach, plum, apricot, nectarine, and cherry, using 2 commercial ELISA‐based almond detection kits (Röder and others ). The cherry species assessed in that case was the sweet edible pulp cherry variety, P. avium .…”

“…It should be noted that peach and apricot kernels are used in a confectionary known as persipan. The 2 ELISA kits tested by Röder and others () are described by the manufacturers as cross‐reacting with apricot kernel protein. In addition, Su and others () reported cross‐reactivity of the anti‐almond 11S legumin mAb 4C10 with P. persica, P. mira, P. webbii, P. argentea, P. fenzliana, P. bucharia , and P. tangutica seed kernel proteins in ELISA, Western blot, and dot blot.…”

Comparison of Laboratory‐Developed and Commercial Monoclonal Antibody‐Based Sandwich Enzyme‐Linked Immunosorbent Assays for Almond (Prunus dulcis) Detection and Quantification

“…(0.5 ppm almond) commercial ELISA kits as well as Neogen Reveal 3D (5 ppm almond), Romer AgraStrip (2 ppm almond), and Tepnel Rapid 3D (1 ppm almond) commercial lateral flow devices (Costa and others ; RomerLabs ). Similar sensitivity has been reported for laboratory‐developed ELISA (0.1 to 1 ppm) (Hlywka and others ; Ben Rejeb and others ), PCR (0.1 to 10 ppm almond) (Köppel and others ; Röder and others ; López‐Calleja and others ; Prieto and others ), MS (3 to 17 ppm almond or 0.1 ppm almond peptide) (Bignardi and others ; Heick and others ; Sealey‐Voyksner and others ), and immunoblot (5 ppm almond protein) (Scheibe and others ) methods for almond detection. Compared to the ELISA method (110 to 120 ppm almond) reported by de la Cruz and others () and the PCR methods (50 to 100 ppm almond) reported by Pafundo and others () and Costa and others (), the 4C10 and MonoTrace ELISAs exhibited better sensitivity.…”

Molecular and structural properties of three major protein components from almond kernel

“…In recent years, studies on almond kernel have rapidly increased, particularly studies on the following aspects: the extraction and properties of almond polyphenols and shell pigments (Omri, Benzina, & Ammar, ; Önal, Uzun, & Pütün, ; Smeriglio et al, ), analysis of almond kernel oil and its components (Kodad et al, ; Zhang et al, ), the almond kernel proteins and their functional properties (Sze‐Tao & Sathe, ), and the determination of almond allergen and the allergic mechanism (Röder, Vieths, & Holzhauser, ; Tiwari et al, ). Previous studies on almond protein have primarily focused on the extraction of total protein and functional analysis, allergy identification, etc., and studies on the structural properties of almond kernel proteins are rarely reported.…”