Comparison of Laboratory‐Developed and Commercial Monoclonal Antibody‐Based Sandwich Enzyme‐Linked Immunosorbent Assays for Almond (<i>Prunus dulcis</i>) Detection and Quantification

Liu, Changqi; Chhabra, Guneet S.; Zhao, Jing; Zaffran, Valerie D.; Gupta, Sahil; Roux, Kenneth H.; Gradziel, Thomas M.; Sathe, Shridhar K.

doi:10.1111/1750-3841.13829

Cited by 8 publications

(3 citation statements)

References 54 publications

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“…Liu, Changqi, et al 2017 developed mAb 4C10-based ELISA for almond detection. This in-house assay and commercially available sandwich ELISA kit were similar in sensitivity (LOD < 1 ppm full fat almond, LOQ < 5 ppm full fat almond) and specificity (no cross-reactivity with 156 tested foods at a concentration of 100,000 ppm whole sample) [ 87 ]. The method developed by Slotwinski, E. et al in 2018 allowed the analysis of almond traces by a direct competitive ELISA in vanilla ice cream, biscuits, pasta, and milk chocolate with an LOD of 0.3 mg/kg and an LOQ of 2.5 mg/kg [ 88 ].…”

Section: Overview Of Nuts and Peanut Allergens Composition And Analyt...mentioning

confidence: 99%

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Luparelli

Losito

Angelis

et al. 2022

Foods

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Consumption of tree nuts and peanuts has considerably increased over the last decades due to their nutritional composition and the content of beneficial compounds. On the other hand, such widespread consumption worldwide has also generated a growing incidence of allergy in the sensitive population. Allergy to nuts and peanuts represents a global relevant problem, especially due to the risk of the ingestion of hidden allergens as a result of cross-contamination between production lines at industrial level occurring during food manufacturing. The present review provides insights on peanuts, almonds, and four nut allergens—namely hazelnuts, walnuts, cashew, and pistachios—that are likely to cross-contaminate different food commodities. The paper aims at covering both the biochemical aspect linked to the identified allergenic proteins for each allergen category and the different methodological approaches developed for allergens detection and identification. Attention has been also paid to mass spectrometry methods and to current efforts of the scientific community to identify a harmonized approach for allergens quantification through the detection of allergen markers.

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Section: Overview Of Nuts and Peanut Allergens Composition And Analyt...mentioning

confidence: 99%

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Luparelli

Losito

Angelis

et al. 2022

Foods

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“…Finally, the ELISA method for nicarbazin was established. The stability of the anti-nicarbazin monoclonal antibody in the ELISA assay was tested with initial experiments measuring the intra-and inter-plate variation coefficients (32). The cross-reaction rate is commonly used as the evaluation criterion.…”

Section: Discussionmentioning

confidence: 99%

Preparation and identification of an anti-nicarbazin monoclonal antibody and its application in the agriculture and food industries

et al. 2022

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Background: As a broad-spectrum drug against chicken coccidiosis, nicarbazine is widely used. The international community has made regulations and requirements on the residue limits of nicarbazine metabolites in chicken. The research reports on the detection methods of nicarbazine residues are mainly based on large-scale instruments such as high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry-mass spectrometry (LC/MS/MS) and so on. However, in the practical monitoring and detection application, the rapid, sensitive, efficient and accurate detection of nicarbazine residues is becoming more and more urgent.Methods: This study aimed to establish an enzyme-linked immunosorbent assay (ELISA)-based method to detect nicarbazin drug residues with high sensitivity and specificity, and wide applicability. Artificial immunogens were prepared by molecular modification synthesis. Nuclear magnetic resonance (NMR) and ultraviolet analyses were conducted to confirm that the correct product was obtained. Monoclonal antibodies were acquired by immunizing mice and preparing hybridoma cells.Results: In this study, 4,4'-dinitrocarbanilide (DNC), a metabolite of nicarbazine, was synthesized and modified to make it have immunogenicity. Fifteen healthy female mice of 6-8 weeks old were immunized in three groups. The successfully immunized mice were screened by serum titer. One mouse with the highest titer was fused and cloned three times, and four positive cell lines were obtained. Nine monoclonal antibodies were obtained from mouse ascites. The best matched antigens and antibodies were screened by an ELISA chessboard method. A detection method of nicarbazine ELISA kit was developed. Our prepared anti-nicarbazin monoclonal antibody had a half-maximal inhibitory concentration (IC 50 ) of 0.825 ng/mL, and the curve range was 0.3-24.3 ng/mL. There was no cross reaction to other six common anti-coccidiosis drugs. The recovery results showed that the fortified recovery of the chicken and duck samples ranged from 74.4-111.7%, the test results of which all met the requirements for veterinary drug residue detection.Conclusions: This method, which uses a specific antibody against the nicarbazin metabolic product DNC, enables rapid quantitative detection. Our new ELISA-based method should facilitate the development of assays to monitor and detect agricultural and veterinary drug residues.

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“…Soluble proteins were extracted in borate saline buffer (BSB) at flour: BSB = 1:10 (w/v). Flours were defatted and subjected to previously reported amandin cryoprecipitation methods (Su et al, 2015(Su et al, , 2017Liu et al, 2017). Soluble protein was determined by Bradford and Lowry methods.…”

Section: Seed Soluble Protein and Immunoreactivitymentioning

confidence: 99%

Redomesticating Almond to Meet Emerging Food Safety Needs

Gradziel

2020

Front. Plant Sci.

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Almond is a desirable and high-quality food source where the presence of nut allergens and a vulnerability to aflatoxin and Salmonella contamination represent threats to consumer safety. In 2019, over 1 billion kg. of almonds, representing over 80% of the world total, were produced in California from a relatively few varieties with a very narrow genetic base. To address emerging needs mandated by cultural and climate changes, new germplasm has been introduced combining peach as well as wild peach and wild almond species. Advanced breeding selections incorporating exotic germplasm into a genetic background compatible with commercial production in California have demonstrated sizable reductions in level of kernel immunoreactivity as well as opportunities for improved control of aflatoxin and Salmonella. Breeding strategies employed include direct selection for reduced kernel immunoreactivity from an introgression enriched germplasm, the integration and pyramiding of resistance to multiple components of the aflatoxin disease-insect complex, and introduction of novel nut and tree traits to facilitate mechanized catch-frame field harvesting to avoid contamination with soil-borne pathogens such as Salmonella and Escherichia coli, as well as agrochemical residues.

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Comparison of Laboratory‐Developed and Commercial Monoclonal Antibody‐Based Sandwich Enzyme‐Linked Immunosorbent Assays for Almond (Prunus dulcis) Detection and Quantification

Cited by 8 publications

References 54 publications

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products

Preparation and identification of an anti-nicarbazin monoclonal antibody and its application in the agriculture and food industries

Redomesticating Almond to Meet Emerging Food Safety Needs

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