Sensitive and Rapid RP-HPLC Quantification of Zoledronic Acid in a Hydroxyapatite-based Nanoparticles

Abstract

The aim of this study was to develop and validate a rapid and sensitive reverse-phase high performance liquid chromatography method for the quantitative determination of zoledronic acid in nanoparticles. The mobile phase was a mixture of methanol (30%) and 7 mM tetra butyl ammonium hydrogen sulphate, 2 mM di-sodium hydrogen orthophosphate and sulphate and 8 mM di-potassium hydrogen orthophosphate (70%), pH adjusted to 3 with trifluoroacetic acid. C18 column was used as a stationary phase. The flow rate was maintained at 1 ml/min. The detection of the constituents was done at 215 nm using an ultraviolet detector. Method validation was executed according to the International Conference on Harmonisation guidelines. The retention time of zoledronic acid was 3.27±0.03 min (n=6). The assay was linear over the concentration range of 200-800 μg/ml. The proposed method was sensitive with the limit of detection and limit of quantification values equal to 200 and 800 µg/ml, respectively. The method was accurate with percent mean recovery from 99.01±1.31 to 100.80±0.094% and precise as percent relative standard deviation values were less than 1%. The method was successfully applied for the estimation of zoledronic acid loading efficiency on hydroxyapatite nanoparticles and in vitro zoledronic acid release profile analysis in hydroxyapatite-based nanoparticles.

Zinc Zeolite as a Carrier for Tumor Targeted and pH-responsive Drug Delivery

“…UV-VIS spectrophotometer UV-2600 (Shimadzu, Japan) was applied for the determination of zoledronate concentration during sorption and release process. Measurements were made in the range of 212-240 nm (λ max = 217 nm) [20,21].…”

Influence of Nursing Effect on the Antiinflammatory Potential of Atorvastatin in Acute Cerebral Infarction

“…Usually, the acute cerebral infarction is mainly caused by atherosclerosis and thrombosis in the arteries supplying blood, making the lumen narrow or even blocked, leading to focal acute cerebral insufficiency. In addition if abnormal objects (solid, liquid and gas) enter the cerebral artery or the neck artery through blood circulation, resulting in the blocking of blood flow, die to sudden reduction in blood flow, brain tissue softening and necrosis in the corresponding innervation area occurs thus causing acute cerebral infarction [4][5][6][7][8][9][10][11][12] . The acute cerebral infarction poses a serious threat to patient's life and it is essential to apply active and effective drug treatments along with scientific nursing intervention programs.…”