Sensitive and rapid isocratic liquid chromatography method for the quantitation of curcumin in plasma

Pak, Yvonne; Patek, Renata; Mayersohn, Michael

doi:10.1016/j.jchromb.2003.08.018

Cited by 69 publications

(43 citation statements)

References 18 publications

(21 reference statements)

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“…It has been reported that curcumin has many pharmacological functions, such as antioxygenation, antibiosis and antitumor [1][2][3] . Methods such as high-performance thin-layer chromatographic (HPTLC) [4][5][6][7] , high-performance liquid chromatography (HPLC) [8][9][10][11] , capillary electrophoresis with amperometric detection (CE-AD) 12 , matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOFMS) 13 , spectrofluorimetry 14,15 , liquid phase tandem mass spectrometry (LC-MS/MS) 16,17 , 1 H NMR 17 , resonance light scattering technique (RLS) 18 and spectrophotometry [19][20][21] have been employed to determine curcumin in a variety of matrixes. However, most of these methods have low sensitivity, need a large number of organic solvents or metal ions.…”

Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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A new ionic liquid (IL) based micro-extraction method was developed for the spectrophotometry and spectrofluorometry determination of curcumin in curry products. ILs can effectively extract curcumin from aqueous solution which were used instead of large-scale organic solvents to reduce environmental pollution and production cost. Results show that the fluorescence of curcumin is considerably enhanced in 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim][PF 6 ]). Under optimized conditions, the absorbance of curcumin is proportional to the concentration of curcumin in the range of 18.4 ng/mL to 494 ng/mL, and the detection limit is 6.1 ng/mL. In addition, the fluorescence intensity of curcumin is proportional to the concentration of curcumin in the range of 0.050 ng/mL to 120.1 ng/mL, and the detection limit is 0.002 ng/mL. Therefore, an efficient spectrophotometry and spectrofluorometry method for the determination of curcumin was established, and the actual concentration in curry products was satisfactorily determined. The content of curcumin in curry sauce were 14.10±0.40 and 14.32±0.37 mg/g when using the spectrophotometry and spectrofluorometry, the content of curcumin in curry powder were 2.50±1.73 and 2.75±1.40 mg/g when using the spectrophotometry and spectrofluorometry.

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Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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“…The analytical procedure used was based on liquid-liquid extraction to obtain sufficiently high recoveries. Significantly lower limit of quantitation was achieved in plasma compared to previously published methods [9,16], with LLOQ as low as 1.0 ng/ml for curcumin and 0.5 ng/ml for piperine. No interfering peaks were observed and no significant peaks were found at the retention times of the analytes using the LLE step for both analytes.…”

Section: Discussionmentioning

confidence: 75%

Validated HPLC–MS/MS Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma

Wang¹,

Zhang²,

Yang³

et al. 2012

Trop. J. Pharm Res

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“…6 It has been found that curcumin can bind to Aβ, and promote degradation of deposited plaques and reduce Aβ-induced neurotoxicity. 7,8 Ono et al proved that curcumin binds to amyloid-β 40 and amyloid-β 42 in a dose-dependent manner, inhibiting further aggregation of amyloid-β 40 and amyloid-β 42 . 9 However, curcumin has poor solubility and is unstable in the neutral and alkaline conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The fluorescence detector wavelength was set at Ex 436 nm/Em 518 nm. 8,27 After blood samples were taken at 2, 5, 15, 30, 45, and 60 minutes, the animals were sacrificed for removal of the brain. The brain tissue was removed and homogenized.…”

mentioning

confidence: 99%

“…6,7 However, the potential therapeutic applications of curcumin were limited by its low bioavailability and a short biological half-life; there also has occurred weak transportation across the BBB via iv administration. 8,10 The curcumin had been entrapped in POs that enhanced drug stability in the blood, and improved curcumin transport into the brain. Compared with the control group, the learning and memory ability of curcumin-Tf/Tet-1-POs group was significantly superior to that of the controls after the third day and was also stronger than that of the Tet-1-POs group.…”

mentioning

confidence: 99%

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A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β<sub>1–42</sub>-injected mice

Jia¹,

Sun²,

Lu³

et al. 2016

IJN

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Due to the impermeability of the blood–brain barrier and the nonselective distribution of drugs in the brain, the therapeutic access to intractable neurological disorders is challenging. In this study, dual brain-targeting polymersomes (POs) functionalized by transferrin and Tet-1 peptide (Tf/Tet-1-POs) promoted the transportation of curcumin into the brain and provided neuroprotection. The modification of the ligands that bind to the surface of POs was revealed by X-ray photoelectron spectroscopy analysis. The cell uptake of a coculture model of mouse brain capillary endothelial cells with neurons showed that the Tf/Tet-1-POs had significant transportation properties and possessed affinity for neurons. The pharmacokinetic analysis showed that the blood–brain barrier permeability–surface efficiency of the Tf/Tet-1-POs was 0.28 mL/h/g and that the brain tissue uptake rate (% ID/g) was 0.08, which were significant compared with the controls ( P <0.05). The curcumin-encapsulated Tf/Tet-1-POs provided neuroprotection and ameliorated cognitive dysfunction in intrahippocampal amyloid-β 1–42 -injected mice. These results suggest that the dual brain-targeting POs are more capable of drug delivery to the brain that can be exploited as a multiple noninvasive vehicle for targeting therapeutics.

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Sensitive and rapid isocratic liquid chromatography method for the quantitation of curcumin in plasma

Cited by 69 publications

References 18 publications

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Validated HPLC–MS/MS Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma

A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β<sub>1–42</sub>-injected mice

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Sensitive and rapid isocratic liquid chromatography method for the quantitation of curcumin in plasma

Cited by 69 publications

References 18 publications

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Validated HPLC–MS/MS Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma

A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-&beta;<sub>1&ndash;42</sub>-injected mice

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A dual brain-targeting curcumin-loaded polymersomes ameliorated cognitive dysfunction in intrahippocampal amyloid-β<sub>1–42</sub>-injected mice